liposomes 11171S0307

Views:
 
Category: Entertainment
     
 

Presentation Description

No description available.

Comments

Presentation Transcript

A SEMINAR ON liposomes BY G.UMADEVI Reg. No: 11171S0307 M.PHARMACY, 1ST Yr (PHARMACEUTICS). :

A SEMINAR ON liposomes BY G.UMADEVI Reg. No: 11171S0307 M.PHARMACY, 1 ST Yr (PHARMACEUTICS) . BHARAT INSTITUTE OF TECHNOLOGY

PowerPoint Presentation:

1. Introduction 2. Classification of liposomes 3. Evolution & Routes of administration 4. Method of preparation 5. Interaction of liposomes with cell 6. Characterization 7. Advantages & Disadvantages 8. Applications 9 . New generation of liposomes 10. List of clinically approved drugs 11. Conclusion 12. Reference CONTENTS

PowerPoint Presentation:

INTRODUCTION Liposomes first discovered by Dr. Alec Bangham at Babraham Institute of Cambridge in 1961. It derives its name from Greek words “ Lipos ”- fat & “Soma”- body Definition Liposomes are simple microscopic concentric bilayered vesicles in which an aqueous core is entirely enclosed by a membranous lipid bilayer mainly composed of natural or synthetic phopholipids .

PowerPoint Presentation:

STRUCTURE OF LIPOSOME Phospholipids are amphiphilic with hydrophilic head & lipophilic tail. Head attracts water,where as long hydro carbon chain- tail repels water.

PowerPoint Presentation:

Liposomes which can carry water soluble drugs in their aq. compartment & lipid soluble drugs in the lipid bilayer .

PowerPoint Presentation:

Lipophilic tail is composed by 2-fatty acid chains containing 10-24 carbon atoms & 6-double bonds in each chain. Polar Head Groups 3 - carbon glycerol & phosphate

PowerPoint Presentation:

Structural parameters Method of preparation Composition & Application CLASSIFICATION OF LIPOSOMES 4. MVV/MV > 1 µm REV MLV-REV FATMLV DRV SPLV VET 1. MLV > 0.5µm 2. OLV – 0.1-1µm 3.ULV – all size a) SUV : 20 - 40 nm b) MUV : 40 – 80nm c) LUV : 100 - 1000nm d) GUV : > 1000 nm Conventional lipo . Fusogenic lipo . pH sensitive lipo . Cationic liposomes Long circulating lip. Immuno liposomes

PowerPoint Presentation:

Conventional Immuno LCL ( Stealth) Cationic pH dependent

PowerPoint Presentation:

EVOLUTION OF LIPOSOMES Early traditional liposomes Antibody targeted immuno liposomes Long circulating liposomes Long circulating immuno liposomes New generation liposomes ROUTES OF ADMINISTRATION Intravenous : Eg : anticancer drugs Oral : Eg : Insulin Intraperitoneal & Intralymphatic : controlled delivery devices in extravascular region when applied directly to the site of action. Aerosol : The use of liposomes in form of an aerosol for treatment of asthma Ocular delivery : Eg : Ketoprofen , Adrenaline Pulmonary delivery : Eg : Salbutamol , Cytarabin Transdermal delivery : Eg : Benzocain , Ketoconazole

PowerPoint Presentation:

Phospolipids : Glycerol containing,saturated & unsaturated PL‘s are used. Natural phospholipids : Phosphatidyl choline (PC) Phosphatidyl ethanolamine(PE) Phosphatidyl serine(PS) Phosphatidyl glycerol(PG) Phosphatidyl acid(PA) Synthetic phospholipids : Dipalmitoyl phosphatidyl choline (DPC) Dipalmitoyl phosphatidyl serine(DPS) Dipalmitoyl phosphatidyl acid(DPA) Dipalmitoyl phosphatidyl ethanolamine(DPE) Materials used for the preparation of liposomes

PowerPoint Presentation:

2. Sphingolipids & glycosphingolipids : Back bone of SL’s is spingosine Zwitter ions at pH-7 Formation of bilayer structure in aq.media Eg : Sphingomylin GSL found in grey matter of brain Steriods : Eg : Cholesterol ↑se rigidity of bilayer , ↓se permeability & leakage. Polymeric material : Eg : PEG forms a protective layer Charge induced substance : Eg : DAG, DAP, Stearylamine

PowerPoint Presentation:

General method of Liposome Preparation

Cholesterol Lipids Charge Dissolve in organic solvent(CHClз,Ethanol,Ether) Solution in organic solvent Drying Thin film Dispersion(hydration) Liposome suspension:

Cholesterol Lipids Charge Dissolve in organic solvent( CHCl з , Ethanol,Ether ) Solution in organic solvent Drying Thin film Dispersion(hydration) Liposome suspension

PowerPoint Presentation:

Preparation of Lipid for Hydration:

PowerPoint Presentation:

LIPOSOME – CELL INTERACTION Adsorption Endocytosis Fusion Drug efflux

PowerPoint Presentation:

Physical Characterization

PowerPoint Presentation:

Chemical Characterization

PowerPoint Presentation:

Biological characterization Aerobic or anaerobic cultures Sterility Pyrogenicity Animal toxicity

PowerPoint Presentation:

Liposomes are biocompatible, biodegradable, non-toxic, flexible & nonimmunogenic for sys. & non- sys.administrations . Delivery of hydrophobic, amphipathic & hydrophilic drugs & agents. Protect their encapsulated drug from the external environment & to act as sustained release depots. It can be formulated as a suspension, aerosol, or in a semisolid form, as a dry vesicular powder for reconstitution. They can be administered through most routes of administration. Encapsulate not only small molecules but also macromolecules. ADVANTAGES OF LIPOSOMES

PowerPoint Presentation:

Liposomes are reduced toxicity & increased stability of entrapped drug via encapsulation. Liposomes are increased efficacy & therapeutic index of drug. Liposomes help to reduce exposure of sensitive tissues to toxic drugs. Alter the pharmacokinetic and pharmacodynamic property of drugs. Flexibility to couple with site-specific ligands to achieve active targeting.

PowerPoint Presentation:

Production cost is high Leakage & fusion of encapsulated drug / molecules. Sometimes phospholipid undergoes oxidation and hydrolysis like reaction Short half-life Low solubility Fewer stables DISADVANTAGES OF LIPOSOMES

PowerPoint Presentation:

THERAPEUTIC APPLICATIONS OF LIPOSOMES 1. Liposomes as drug/protein delivery vehicles * Controlled & sustained drug release in situ *Enhanced drug solubilization 2. Liposomes in antimicrobial, antifungal & antiviral therapy *Liposomal drugs *Liposomal biological response modifiers 3. Liposomes in tumour therapy *Carrier of small cytotoxic molecules *Vehicle for macromolecules as cytokines or genes

PowerPoint Presentation:

4. Liposomes gene delivery *Genetic vaccination *Gene & antisense therapy 5. Liposomes in immunology * Immunomodulator * Immunoadjuvant 6. Liposomes as artifial blood surrogates 7. Liposomes in cosmetics & dermatology 8. Liposomes as radiopharmaceutical & radio-diagnostic carriers

PowerPoint Presentation:

New generation of liposomes

PowerPoint Presentation:

Name Trade name Company Indication Liposomal amphotericin B Abelcet Enzon Fungal infections Liposomal daunorubicin DaunoXome Gilead Sciences HIV-related Kaposi’s sarcoma Liposomal cytarabine Depocyt Pacira (formerly SkyePharma ) Malignant lymphomatous meningitis Liposomal doxorubicin Myocet Zeneus Combination therapy with cyclophophamide in metastatic breast cancer Liposomal morphine DepoDur SkyePharma , Endo Postsurgical analgesia Liposomal verteporfin Visudyne QLT, N ovartis Age-related macular degeneration, pathologic myopia List of clinically approved liposomal drugs

PowerPoint Presentation:

CONCLUSION Liposomes are one of the unique drug delivery system, which can be of potential use in controlling & targeting drug delivery. Now adays liposomes are used as versatile carriers for targeted delivery of drug. Novel drug delivery systems had more impact on pharmaceutical industry from the last decade. Many marketed formulations of liposomes have been approved every year & there is increased demand for them. Many noval liposomal types are waiting for regulatory approval to enter into the market.

PowerPoint Presentation:

1. S.P.Vyas & R.K.Khar ., Targeted & Controlled Drug Delivery Novel Carrier Systems, 2002; 173-243. 2. N.K.Jain ., Advances in Controlled & Novel Drug Delivery, 2001; 50. 3. Bangham A. D., Standish M. M., and Watkins J. C., J. Mol. Biol., 1965;13:238-252. 4. Suggy S. Chrai , R. Murari , Imran Ahmad., Pharm. Technology, 2002;26:28-34. 5. Dean J. A. Crommelin , Gert W. Bos , Gert Storm., Business briefing pharmatech , 2003; 209-213. 6. Dass C. R., Burton M. A., J. Pharm. Pharmcol . , 1999;51:755-770. 7.  http://www.uni-magdeburg.de/imos/mea_sen/img/pictures 8.  http://www.nanolifenutra.com/images 9.  http://www.azonano.com/work/C2In_files 28 BIBLIOGRAPHY 28 BHARAT INSTITUTE OF TECHNOLOGY

authorStream Live Help