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Repeated cocaine administration induces gene expression changes through Dopamine D1 Receptors:

Repeated cocaine administration induces gene expression changes through Dopamine D1 Receptors Presented by: Mariam D’ippolito and Mesut Ozer (u1148511, u1115120) Dopamine

Introduction:

Introduction Drug addiction: what is it? Effects of cocaine on dopamine receptors Permanent alterations in the brain Some alterations are changes in gene expression Cocaine

Methods: RNA Isolation:

Methods: RNA Isolation Mice were injected with cocaine for a period of 7 days The RNA of the mice injected with cocaine and the control groups were isolated, then quality was examined through optical density readings and gel electrophoresis.

Methods: Data analysing/processing:

Methods: Data analysing/processing RNA isolated Microarray processing used to analyse RNA and compare cocaine to non-cocaine individuals

Results:

Results 109 genes expressed a difference between cocaine mice and saline mice 1.2 fold difference No gene differences between saline and wild type mice The D1 receptors were either down or up-regulated compared to wild type mice Differential gene expression in the CPu in D1 receptor mutant and wild-type mice after repeated exposure to cocaine. D1 receptor mutant and wild-type mice. Y-axes represent relative expression levels. *po0.05 and w po0.05 between and within groups, respectively. 0.05oz po0.1 within group. The D1 receptor gene was used as a control.

Conclusion:

Conclusion There is a molecular basis for behavioural changes from cocaine use. Changes in brain are permanent Responsiveness of D1 receptor expressing neurons change and will act differently to further neuronal stimulation.

References:

References http://www.nature.com/npp/journal/v30/n8/full/1300680a.html Thank you for listening.