collection of specimen for fungal infections

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Collection of specimen for fungal infections. : 

Collection of specimen for fungal infections. Nuzhath fatima. Department of Microbiology Faculty of Applied Medical Sciences. Jazan University, Jazan,KSA

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“A properly collected and properly handled specimen is a prerequisite for laboratory diagnosis” The most important steps for the isolation of fungus are: proper collection of specimens. rapid transport to the lab. prompt and correct processing of the specimen. inoculation onto appropriate media. incubation at suitable temperatures.

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A. Skin scrapings: Grossly contaminated skin should be cleaned with 70% alcohol and then with water. A curved disposable scalpel blade is used in scapping across the inflamed margin of the lesion.

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Nail: Scrap the nail with scalpel and discard the first superficial scrapping. Some of the friable material from under nail may be used.

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Hair: Infected hair removed by plucking with forceps. Both the hair shaft and the hair root should be obtained. Note: infected hair may fluoresce under ultra-violet light (wood’s lamp), which suggests the etiologic agent may be microsporum. Non-fluorescent hair should also be examined under a wet mount, 10% KOH and cultured, if a fungus is suspected clinically.

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Sputum: Sputum specimen is submitted in case of systemic infections. An early morning specimen is desirable. The patient must brush his teeth, rinse his mouth thoroughly and raise the sputum by coughing. Collect the sputum in a clean, sterile container with a tight-fitting lid.

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Sputum is first digested in pancreatin solution (1%, in phosphate buffer, PH 7.5) for about 2 hours at 350C, or until liquefied (shake occasionally). Centrifuge the liquefied specimen at 3000 rpm for 20 minutes and use the sediment for inoculation and direct microscopic examination.

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Pus and exudates: Pus specimens are taken from mucocutaneous ulcers, draining subcutaneous sinuses, and from abscesses. Cotton swabs are seldom satisfactory. A draining pus specimen helps considerably in examining a diagnosing the causing agent by direct microscopic examination.

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Since the surface contaminants may lead to erroneous results, the abscess is first cleansed with 70% alcohol, then cleaned with tincture of iodine, and then cleaned with sterile water. Pus is first aspirated with a sterile needle and syringe before the incision is made. Remove the aspirate with a sterile Pasteur pipette, and place it in a sterile labeled test tube

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CSF: For suspected cases of mycotic meningitis, CSF is submitted for laboratory investigation. Cryptococcus neoformans is most commonly responsible for this clinical condition. The organism is delicate and occurs in small numbers in the spinal fluid the spinal fluid is spun at low speed, and the sediment is used for microscopic examination and culture.

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Blood specimens: Blood specimens, used for the bacterial culture may show the growth of organisms which stay in the yeast phase within the body. Urine sediment is used to examine some of the yeast forms of systemic mycoses. Collect the first morning specimen aseptically as for any urine culture A clean catch midstream urine is acceptabl.e

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Thank you.

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