Bacteriophages new isolation technique

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Exhibiter : Jafet Salomón Valencia Ospina

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Introduction Phages are virus They go for bacteria Are becoming an alternative for the multi-drug resistant bacterias Have been discovered in late XIX century

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Phages classification Bacteriophages cycle

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Aim of the study Develop a method for selective isolation of large phages such as jumbo phages (with a genome larger than 200 kbp ) and phages with a genome around 200 kbp including T4- like phages Jumbo phages because there’s no standard method to isolate them, more of the obtained nowadays are smaller

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Materials and Methods Ilustrating sampling of wastewater Culture of 3 different strains of Gram positive bacteria and 26 of Gram negative bacteria Recolection of wastewater samples Purification of phages Dosification of purified phages over plates with cultured bacteria

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PFGE Pulse Field Electrophoresis Phage DNA was purified by centrifugation Obtained particles were put on gel and treated with proteinase Difference between normal electrophoresis and PFGE is direction of the voltage field

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Results E9 phage has got most host range This method against previous increases significatively the phages plaques

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Discussion Ross A. Et al: Phages are usually species specific and even strain specific Handi S. Et al: Shigella SH7 (phage) infects strains of E. coli, Salmonella Paratyphi and Shigella dysenteriae Kȩsik-Szeloch , A. Et al (AND) Roszniowski , B. Et al: Some large phages may not be obtained because of their sensibility to Chloroform

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Conclusions This modified method improves over the standard in the obtainment of jumbo bacteriophages plaques, allowing its systemic isolation and use for bacterial control of certain strains Some phages show specificity to a group of bacteria whilst others (E4, E9) have a wider host variety, and because their DNA patterns vary, there could be a relationship between both

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