Module 9.9 Part C

Views:
 
Category: Entertainment
     
 

Presentation Description

No description available.

Comments

Presentation Transcript

Curved Gram-negative rods and spirochetes:

Curved Gram-negative rods and spirochetes Module 9.9 Part C

Borrelia:

Borrelia

Brindle, a lame dog:

Brindle, a lame dog Brindle is 5-year-old Miniature Schnauzer from Leamington, near Point Pelee Loves to go into the Carolinian forest Late summer became dull, depressed, anorexic, disliked exercise, panting on exercise? Three weeks later lame on both front legs, humeral-carpal joint very swollen

Owners remember pulling a large tick off her in late July:

Owners remember pulling a large tick off her in late July

Brindle has Lyme disease:

Brindle has Lyme disease

Borrelia:

Borrelia Gram-negative spiralled rods Arthropod (usually tick -borne) infections Relapsing fever people ( B. recurrentis ) Borrelia burgdorferi most significant in Canada, unusual Problem in eastern, central, western US associated with resurgent deer populations (source of food for adult ticks)

PowerPoint Presentation:

Borrelia burgdorferi infection

Borrelia burgdorferi:

Borrelia burgdorferi Agent of Lyme disease Tick-borne infection in much of US Vector is 3-host tick Ixodes scapularis (black-legged “deer” tick) in eastern/central USA, I. pacificus in western US, SW British Columbia Deer major source adult ticks Populations along north shore lakes Erie and Ontario, but spreading in Canada Main host white-footed mouse, many bird species: all persistently bacteremic

PowerPoint Presentation:

The distribution of I xodes scapularis ticks submitted to PHAC by the public (red circles that are centred on the centroid of the census sub-division from which they were submitted.) (Lindsay, unpublished)

Borrelia burgdorferi:

Borrelia burgdorferi Spread to animals and people mostly from nymphal tick bite In people chronic erythematous skin rash, followed by fever, muscle and joint ache, meningeal irritation Weeks or months later peripheral neuropathy, arthritis, myocarditis

Borrelia burgdorferi:

Borrelia burgdorferi Dogs: Fever, anorexia, arthritis Horses: Arthritis, abortion, panuveitis, encephalitis Diagnosis: History, clinical signs, serology, prompt response to antibiotics Difficulty diagnosing in endemic areas since subclinical infection (exposure) common Treatment: Vaccine in endemic areas (antigenic variants issue)

Helicobacter:

Helicobacter

Kee Kee keeps vomiting:

Kee Kee keeps vomiting

Kee Kee keeps vomiting:

Kee Kee keeps vomiting Kee Kee is a slight, underweight one year old cat Despite hair-ball preventions, Kee Kee often vomits after feeding Owner “wants to get to the bottom of problem” Biopsied for gastritis

Kee Kee keeps vomiting:

Kee Kee keeps vomiting Kee Kee has mild diffuse chronic gastritis Silver stain shows Helicobacter -like bacteria

Kee Kee keeps vomiting:

Kee Kee keeps vomiting Kee Kee may have Helicobacter gastritis Owner refuses PCR to confirm species Two months of oral amoxicillin do not resolve the gastritis Kee Kee is still vomiting, but less so since owners feed him differently

Helicobacter:

Helicobacter Fastidious Gram-ve microaerophilic curved rods Stomach (sometimes intestines) animals and humans Host species adaptation May cause chronic superficial gastritis H. pylori humans (“type disease”); gastritis, ulceration, even gastric carcinoma

Helicobacter:

Helicobacter Explosion of interest in genus in animals In some cases associated with gastritis, hepatitis, diarrhea In many cases commensals Some may be important as zoonoses

Helicobacter: just some examples:

Helicobacter : just some examples H. felis Dog, cat Stomach Gastritis exptally H. heilmanii Dog, cat, pig (human) Stomach Gastric ulcer swine?? H. canis Dog, human Intestine Hepatitis dog H. hepaticus Mice (A/JCR) Intestine Hepatitis, adenoma H. bovis Cow Abomasum Disease?? H. acinonyx Cheetah Stomach Gastritis

Helicobacter:

Helicobacter “Watch this space bacterium” Diagnosis: Gastritis: Biopsy, Gram, urease assay on biopsy; isolation; PCR; ELISA; isolation (tough); 16sRNA PCR and sequence

Brachyspira and Serpulina:

Brachyspira and Serpulina

Brachyspira and Serpulina:

Brachyspira and Serpulina B. hyodysenteriae Swine dysentery B. pilosicoli Intestinal spirochetosis: Swine, dogs, ducks, chickens, humans B. alvinpulli Enteropathogen chickens B. innocens Commensal pigs S. intermedia, S. murdochii Commensal pigs

Brachyspira and Serpulina:

Brachyspira and Serpulina Anaerobic intestinal spirochetes 5-10 x 0.3 um, snake like spirochetes Grow 3-5 days selective media; PCR used in diagnosis Different species pathogens or commensals Most important in swine Understanding still emerging

Brachyspira hyodysenteriae:

Brachyspira hyodysenteriae Agent of “swine dysentery” Contagious mucohaemorrhagic fibrinonecrotic colitis of weaned pigs Causes weight loss, impaired growth; variable mortality

Brachyspira hyodysenteriae:

Brachyspira hyodysenteriae Diagnosis: PCR; culture (difficult) Control: Now unimportant, because of three site production Does not persist in clean environments Antimicrobial drugs

authorStream Live Help