Restriction Enzymes Action

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Slide 1: 

Bacterium chromosome Plasmid Restriction Enzymes Restriction enzymes are isolated from bacteria 2. They identify a particular sequence EcoRI 3. And make a cut at a specific site

Slide 2: 

EcoRI EcoRI produces sticky ends/overhangs

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Some RE produces Blunt Ends DNA fragments fused with sticky ends have more firm binding than blunt ends

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Bacterium RE Why RE do not degrade/cut bacterial (host) DNA? Because RE sites in the host DNA are METHYLATED

Slide 5: 

Types of RE Type I Type II Type III

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