Cellular hematological profiling for Cancer detection

Cellular haematological profiling: 

Cellular haematological profiling A Presentation By Yogesh Srivastava Senior Research Fellow (Transgenic) Indian Institute of Vegetable Research Varanasi Supporting Guide: Dr. Sunil Chaudhary (M.B.B.S., M.S.), Assistant Professor BHU Project Guide: Co-Guide: Dr. Major Singh Dr. Shailesh K. Tiwari Principle Scientist Scientist Crop Improvement Division Crop Improvement Division IIVR, Varanasi IIVR, Varanasi

Cellular haematological profiling : 

Cellular haematological profiling Key questions: How it can be done? What procedure would help to identify targets? What would be the benefit of all these activities? Perceived benefits in disease diagnosis and prognosis? Will it reduce the cost of clinical treatment?

Slide 3: 

Predicted model for cellular hematological profiling

The methodology?: 

The methodology? Blood serum from patients could be a good starting material. DNA/RNA isolation and sequencing of all relevant genes. BLAT (BLAST-like alignment tool) to compare for cancerous genes. Phylogenetic analysis using MSA (Multiple sequence alignment) Utilize universal microsatellite markers for DNA/RNA transposons. Perform RT-PCR and electrophoresis. In silico gene expression analysis by GENE SPRING and Microarray express. Expressed protein has to be retrieved after expression analysis. Use a methylated ligand for docking into that protein. Studies on docking pattern, entropy, energy changes, and ligand designing may help in treatment.

Phylogenetic analysis: Evolutionary relationships between different cancer associated genes: 

Phylogenetic analysis: Evolutionary relationships between different cancer associated genes

Phylogenetic analysis in model organisms: 

Phylogenetic analysis in model organisms

Structural analysis of expressed protein: 

Structural analysis of expressed protein

Ligand designing by ArgusLab: 

Ligand designing by ArgusLab Ligand and protein both can be design by ArgusLab. Active Domain can get from Emboss Antigenic server to design.

Protein structure validation using Ramachandran Plot: 

Protein structure validation using Ramachandran Plot

3D Molecular Docking performed by HEX: 

3D Molecular Docking performed by HEX Docking indicates that ligand is perfectly bound to receptor Binding can suppress the over-expression of protein.

ADME (Adsorption, Distribution, Metabolic, Excretion) Test: 

ADME (Adsorption, Distribution, Metabolic, Excretion) Test ADME test to prove the utility of ligand

ToxBox Test to predict the toxic effect of Ligand to different organs: 

ToxBox Test to predict the toxic effect of Ligand to different organs

Proposed Kit for Cellular haematological profiling : 

Proposed Kit for Cellular haematological profiling It has methylated ligand-coated paper; when serum sample is put in the kit, the ligand will bind to the receptor. The coloring reagent will make sure binding of receptor and ligand. Sample from diseased person would show colorless/dim color.

Perceived benefits : 

Perceived benefits True benefit of this activity to reduce the time of research and make easy to test disease. It helps to pathologist/clinicians in disease diagnosis and prognosis by saving time.

Slide 15: 

Thank you