transgenic plant as bioreactor for recombinant protein

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how plant act as bioreactor and produce r protein

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Transgenic plant as bioreactor of recombinant protein:

Transgenic plant as bioreactor of recombinant protein Made by Vikas kumar singh 2 nd m.sc. biotechnology

CONTENT:

CONTENT What is a bioreactor ? WHY PLANTS ARE USED AS BIOREACTORS ? COMPARISON WITH OTHER PRODUCTION SYSTEM PRODUCTION OF PROTEINS FROM PLANTS Transgenic plant used for production products Transgenic plants advantages Transgenic Plant Using As A Host Recent developments

What is a bioreactor ?:

What is a bioreactor ? A device in which a substrate of low value is utilized by living cells to generate products of higher value . Plants are exploited as bioreactors for the production of biomolecules.

Transgenic plant as bioreactor:

Transgenic plant as bioreactor Genetically modified plants can be manipulated to act as bioreactors to produce a wide range of biologically important compounds. These include carbohydrate, lipids and proteins, besides secondary products. The commercial products of plants are useful for industries or for improving the human or animal health.

WHY PLANTS ARE USED AS BIOREACTORS ? :

WHY PLANTS ARE USED AS BIOREACTORS ? Post translational modifications Storage costs Ethical considerations

COMPARISON WITH OTHER PRODUCTION SYSTEM :

COMPARISON WITH OTHER PRODUCTION SYSTEM Low cost alternative Post translational modifications Storage facilities Low upstream production cost

PRODUCTION OF PROTEINS FROM PLANTS :

PRODUCTION OF PROTEINS FROM PLANTS Expression of peptide relies on: a) Stable intergration of transgene b) By transient expression of genetically engineered viruses

Transgenic plant used for production products:

Transgenic plant used for production products Plants such as tobacco, potato, tomato, banana, legumes, and cereals as well as alfalfa, are used in molecular farming and have emerged as promising biopharming systems for production of pharmaceutical proteins, such as antibodies, vaccines, regulatory proteins and enzymes. Tobacco, Potato, Tomato, Banana, Legumes, And Cereals As Well As Alfalfa Antibodies, Vaccines, Regulatory Proteins And Enzymes Used In Molecular Farming

Transgenic plants advantages:

Transgenic plants advantages Low Cost Of Cultivation, High Biomass Production, Relatively Fast Protein Synthesis, Low Operating Costs, Excellent Scalability, Eukaryotic Posttranslational Modifications, Low Risk Of Pathogenicity Toward Humans And Endotoxins, And A Relatively High Protein Expression Level

Examples of therapeutic antibodies produced in plants:

Examples of therapeutic antibodies produced in plants

Transgenic Plant Using As A Host:

Transgenic Plant Using As A Host Using transgenic plants as a host is highly attractive in that proteins can be administered in fruits and vegetables as a source of antigens for oral vaccination. Thus, potatoes expressing a synthetic LT-B gene, labile toxin from Escherichia coli were successfully used in a clinical study to examine an edible plant vaccine and hepatitis B. No plant-derived protein has still been developed to be used as a drug, but molecular farming has gained attention as plants can be turned into medicine factories.

Recent developments:

Recent developments Plant cells have also been considered to be an alternative host for the production of recombinant proteins since they are able to glycosylate proteins. Of the various systems used for cultivation, such as hairy roots, immobilized cells, and free cell suspensions, the latter is regarded to be most suitable for large-scale applications. Full-size antibodies, antibody fragments, and fusion proteins can be expressed in transgenic-plant cell systems

Reference:

Reference Pharmaceutical biotechnology by oliver kayser – page no 29 B iotechnology by U.satayanarana – pg no 624 www.srmuniv.ac.in/downloads/plant-biorector

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