SHEEP BLOOD AGAR BASE, MODIFIED PLATE

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Sheep Blood Agar Base was formulated to be compatible with sheep blood and give improved haemolytic reactions of organisms. BACILLUS CEREUS is Gram -positive aerobic or facultatively anaerobic, motile, spore forming, rod shaped bacterium that is widely distributed environmentally. B. CEREUS is associated mainly with food poisoning it is increasingly reported to be cause of serious and fatal non- gastointestinal-tract.

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TM Media  +91-11-71239900  +91-9999-168-770  marketingtitanbiotechltd.com  904 9th Floor Bigjos Tower   Netaji Subhash Place   Delhi 110034  SHEEP BLOOD AGAR​ BASE MODIFIED PLATE    Dehydrated Culture Media  INTENDED USE  for cultivation and studying haemolytic reactions of Bacillus cereus in accordance with  ISO 21871:2006.     Composition     Ingredients  Gms/Ltr.   Enzymatic digest of casein   15.000  Agar  12.500  Sodium chloride   5.000  Enzymatic digest of soya   5.000

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2     PRODUCT SUMMARY AND EXPLANATION  Sheep Blood Agar​ Base Modified with added sheep blood was developed to allow  maximum recovery of B. cereus without interfering with their haemolytic reactions. This  medium is formulated in accordance with ISO. It was formulated to be compatible with  sheep blood and give improved haemolytic reactions of organisms.   Bacillus cereus is Gram -positive aerobic or facultatively anaerobic motile spore  forming rod shaped bacterium that is widely distributed environmentally. B. cereus is  associated mainly with food poisoning it is increasingly reported to be the cause of  serious and fatal non- gastrointestinaltrakt.   Hemolysis is very useful for differential characteristics that is best viewed when a bright  light is transmitted from behind the plate. Four different types of hemolysis can be  described:   1. Alpha-hemolysis α – Partial hemolysis that results in a greenish discoloration  around the colony.   2. Beta-hemolysis β – Complete lysis of red blood cells resulting in a clear zone  around the colony.    3. Gamma-hemolysis γ – No hemolysis resulting in no change in the medium.   4. Alpha-prime-hemolysis α′ – A small zone of complete hydrolysis that is surrounded  by an area of partial hemolysis.    PRINCIPLE  Medium contains nutritional components like enzymatic digest of casein enzymatic digest of  soya and the addition of sodium chloride provides an osmotically balanced medium for bacterial  cells. The addition of 5 defibrinated sheep blood allows for the determination of hemolytic  reactions an important differential characteristic.

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3   INSTRUCTION FOR USE  Either streak inoculate or surface spread the test inoculum aseptically on the plate.  QUALITY CONTROL SPECIFICATIONS   Appearance:​ Cherry red coloured opaque gel forms in Petri plates.   Quantity of Medium​: 25ml of medium in 90mm plates. .  INTERPRETATION   Cultural characteristics observed after incubation at 30-35 °C for 18-48 hours  Microorganisms  ATCC  Inoculum  CFU/ml   Growth  Recovery  Rate   Colour of  colony  Bacillus cereus  10876  103   Luxuriant  70   Beta    STORAGE STABILITY   On receipt store the plates at 2–8 °C. Avoid freezing and overheating. Do not open until ready to  use. Prepared plates stored in their original sleeve wrapping until just prior to use may be  inoculated up to the expiration date and incubated for recommended incubation times. Allow  the medium to warm to room temperature before inoculation.   Product Deterioration:​ Do not use plates if they show evidence of microbial contamination  discoloration drying cracking or other signs of deterioration.  REFERENCES  1. MacFaddin JF. Media for isolation-cultivation maintenance of medical bacteria Vol I.  Baltimore: Williams Wilkins 1985.   2. Isenberg HD Ed. Clinical microbiology procedures handbook. Washington DC: ASM 1992.   3. Kramer JM Gilbert RJ. Bacillus cereus and other Bacillus species. In: Doyle MP ed.  Foodbome Bacterial Pathogens New York: Marcel Dekker 1989 21-70

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4   4. NCCLS. Quality assurance of commercially prepared microbiological culture media. 2nd  ed. NCCLS document M22-A2. Wayne PA: NCCLS 1996.   5. Forbes BA Sahm DF Weissfeld AS. Bailey and Scott’s diagnostic microbiology. 10th ed. St.  Louis: Mosby 1998. 5. Pelczar M. J. Jr. Reid R. D. Chan E. C. S. 1977 Microbiology 4th Ed.  Tata McGraw-Hill Publishing Company Ltd New Delhi.    6. Koneman E. W. Allen S. D. Janda W. M. Schreckenberger P. C. Winn W. C. Jr. 1992 Colour  Atlas and Textbook of Diagnostic Microbiology 4th Ed. J. B. Lippinccott Company.   7. International Organization for Standardization ISO Draft ISO 21871:2006 Microbiology of  Food Animal feeding stuffs. Horizontal method for the determination of low numbers of  presumptive Bacillus cereus-Most probable number technique and detection method

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