Technical Datasheet of Sabouraud Dextrose Agar or SDA

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Sabouraud Dextrose Agar or SDA contains Mycological peptone provide nitrogen and vitamin source required for organism growth. Due to its low pH this medium is very sensitive to overheating which will soften the agar and caramelize the carbohydrate. Agar is the solidifying agent.


Presentation Transcript

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TM Media  +91-11-71239900  +91-9999-168-770   904 9th Floor Bigjos Tower   Netaji Subhash Place Delhi 110034  Sabouraud Dextrose Agar  Dehydrated Culture Media  INTENDED USE  Sabouraud Dextrose Agar​ for cultivation of yeasts molds and aciduric microorganisms  COMPOSITION    Ingredients  Gms/Ltr.  Dextrose   40.000   Agar  15.000   Peptone  10.000     PRODUCT SUMMARY AND EXPLANATION  Sabouraud Dextrose Agar is Carliers modification of the formulation described by  Sabouraud for the cultivation of fungi yeasts moulds particularly useful for the fungi  associated with skin infections. George enhanced SDA with the addition of  cycloheximide streptomycin and penicillin to produce an excellent medium for the  primary isolation of dermatophytes. This medium is also employed to determine

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2   microbial contamination in food cosmetics and clinical specimens. Sabouraud  Dextrose Agar is recommended by the U.S.  Pharmacopeia for microbial limit tests.    PRINCIPLE  Mycological Peptone provides nitrogenous  compounds. Dextrose provides an energy  source. High dextrose concentration and low pH  favours fungal growth and inhibits contaminating  bacteria from test samples.      INSTRUCTION FOR USE   1. Dissolve 65.0 grams in 1000 ml distilled water.   2. Gently heat to boiling to dissolve the medium completely.   3. Sterilize by autoclaving at 15 psi 121°C for 15 minutes.   4. Cool to 45-50°C.   5. Mix well and pour into sterile Petri plates.  QUALITY CONTROL SPECIFICATIONS  Appearance of Powder:​ Cream to yellow colour homogeneous free flowing powder.  Appearance of prepared medium:​ Light amber colour clear to slightly opalescent gel   pH at 25°C : ​5.6 ± 0.2

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3     INTERPRETATION:  Cultural characteristics observed after inoculating 50-100 CFU for yeast diluted culture  incubation for 18-48 hours or up to 4 - 6 days at 25-30°C. If necessary at 30 ± 2°C for 3  weeks. For molds point inoculation for incubation at 25-30°C for upto 5-7 days.  STORAGE STABILITY :  Dehydrated powder hygroscopic in nature stored in a dry place in tightly-sealed  containers below 25°C and protected from direct Sunlight. Under optimal conditions the  medium has a shelf life of 4 years. When the container is opened for the first time note  the time and date on the label space provided on the container. After the desired  amount of medium has been taken out replace the cap tightly to protect from hydration.     Microorganisms   ATCC  Inoculum  CFU  Growth  Appearance of  colony  Standard  recovery   Aspergillus brasiliensis   16404   Point  Inoculation  Luxuriant  White mycelium  black spores  Good zone  diameter  Trichophyton  mentagrophytes   9533  Point  Inoculation  Luxuriant  White to cream  colour downy to  fluffy  Good zone  diameter  Candida albicans  10231  50-100  Luxuriant  Whitish convex  entire dimorphic  ≥ 70  Candida krusei  6258  50-100  Luxuriant  Greyish white  flat circular  dimorphic  ≥ 70  Saccharomyces cerevisiae  9763  50-100  Luxuriant  White to cream  flat smooth  moist glistening  ≥ 70

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4   or dull glabrous  Penicillium corylophilum  20203  Point  Inoculation  Luxuriant  Greyish green   Good zone  diameter  Trichoderma viride   52440  Point  Inoculation  Luxuriant  Cottony  bluish-green  Good zone  diameter

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