Enzyme-immobilization

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ENZYME IMMOBILIZATION:

ENZYME IMMOBILIZATION PRESENTED BY- Swapna sharma M.Phil(Microbiology) Faculty of life science MATS UNIVERSITY

SYNOPSIS :

SYNOPSIS INTRODUCTION METOHDS OF IMMOBILIZATION ADSORPTION COVALENT BINDING CROSS LINKING ENTRAPMENT IMMOBILIZATION OF MICRO ORGANISM AND CELLS COMPARISON BETWEEN METHODS NEW DEVELOPMENTS REFERENCES FUTURE PROSPECTS

Introduction:

Introduction Enzymes have many advantages over their chemical counterparts in that they are more specific, and generally posses high catalytic properties. Enzymes can be immobilized i.e., an enzyme can be linked to an inert support material without loss of activity which facilitates reuse and recycling of the enzyme. Enzymes can also be encapsulated or entrapped.

Immobilization :

Immobilization Immobilization can be defined as the process whereby the movement of enzymes, cells, organelles, etc. in space is completely or severely restricted usually resulting in a water-insoluble form of the enzyme. Immobilized enzyme are also sometimes referred to as sound, insolubilized, supported or matrix-linked enzymes.

Slide 5:

R e a s o n s reuse of enzyme (reducing cost) easy product separation continous processing facilitated process control optimisation of product yield stabilization by immobilisation Need for Immobilization Immobilized enzymes are easy to handle.

Limitations :

Limitations cost of carriers and immobilisation changes in properties (selectivity) mass transfer limitations problems with regeneration problems with multienzymes systems activity loss during immobilisation

Slide 10:

disadvantage

METHODS OF IMMOBILIZATION:

METHODS OF IMMOBILIZATION

Crosslinking :

Crosslinking Crosslinking is the another option of enzyme immobilisation Polyfunctional reagents form bridges to form backbone to hold enzyme molecules Eg: glutaraldehyde, benzidiene etc.

New developments :

New developments In addition to tailor fitted route design and designer enzymes, more companies including enzyme producers are now offering an increasing range of immobilized enzymes with a wide choice of enzymes and carriers with particle sizes from micrometers up to millimeters suiting both batch and continues processes. Most of the enzyme carriers used are readily available on the market and guarantee minimal costs and bulk availability .

References :

References U. Satyanarayana- biotechnology Trevor Palmer- Enzymes: biochemistry Horwood Publishing 2001 Robert k murray, D.K. granner- Harper's Illustrated Biochemistry Nureddin A, Inagami T, Biochemistry Journal, vol. 147, 1975, pp. 71-81. Schöneich C, “Kinetics of thiol reactions,” Methods in Enzymology, vol. 251, 1995, pp. 45-55. http://www.chiralvision.com/pdf/carrier-bound_enzymes_chiralvision.pdf

Future of enzyme immobilization:

Future of enzyme immobilization As methods for the immobilization of enzymes continue to improve and become commercially widespread, the availability of immobilized enzymes to industry has the opportunity to increase significantly. This increase in the availability of immobilized enzymes would allow for a growth in the application of immobilized enzymes throughout the chemical and medical fields. These improvements will likely result in a growth in the application of immobilized enzymes to new fields

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