bioremediation of shrimp culturing waste

ANOXYGENIC BIOREMEDIATION OF NITROGENOUS FISH CULTURING POND EFFLUENT : 

ANOXYGENIC BIOREMEDIATION OF NITROGENOUS FISH CULTURING POND EFFLUENT Project submitted to SRM UNIVERSITY By K.V.SRIVATSAN 30405523

INTRODUCTION : 

INTRODUCTION Aquaculture is cultivating of aquatic organisms in controlled conditions. Aquaculture in other words is referred as aquafarming which also includes algaculture, fish farming, shrimp farming, shellfish farming and growing of cultured pearls. Mariculture refers to aquaculture practiced in marine environments. Evidences for aquaculture were traced back to 2500 B.C.

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Bioremediation is defined as the process whereby organic wastes are biologically degraded under controlled conditions to an innocuous state, or to levels below concentration limits established by regulatory authorities. For bioremediation to be effective, microorganisms must enzymatically attack the pollutants and convert them to harmless products. Microorganisms that can degrade various pollutants have been isolated with the eventual goal of exploiting their metabolic potential for the bioremediation.

Advantages of Bioremediation : 

Advantages of Bioremediation Bioremediation is a natural process and the residues for the treatment are usually harmless products and include carbon dioxide, water, and cell biomass. The microbial load first increases when inoculated and the decrease gradually after depletion of waste. Bioremediation is often carried out on site, without causing a major disruption of normal activities in most cases. Cost effective than any other method of waste treatment processes

Objectives : 

Objectives To speed up the natural process of biodegradation of the fish culturing pond effluent using naturally occurring microorganism. To find a solution to minimize the amount of waste present, before discarding into the environment. To find the reduction rate of the total solids present in the effluent observed for a period of two weeks and at an interval of three days.

Methods : 

Methods Sample collection Isolation of phototropic organism Batch culture technique Estimatiom of physiochemical parameters. Results are noted and analysed.

Physiochemical parameters : 

Physiochemical parameters pH Total Solids(T.S) Total Dissolved Solids (T.D.S) Total Suspended Solids (T.S.S) Biological Oxygen Demand (B.O.D) Chemical Oxygen Demand (C.O.D) Dissolved oxygen (D.O)

Result : 

Result Isolation of anoxygenic photosynthetic organism Bioremediation After 15 days

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COMPARATIVE TABLE FOR BATCH STUDIES

GRAPHICAL REPRESENTATION OF REDUCTION IN PHYSIOCHEMICAL : 

GRAPHICAL REPRESENTATION OF REDUCTION IN PHYSIOCHEMICAL

Discussion : 

Discussion In an attempt to develop a cost effect waste water treatment system, efforts have been made to treat aquaculture pond effluent by anoxygenic phototrohic bacteria. The shrimp pond effluents contains high amount of organic substance as a source for eutrophication of costal areas. The untreated aquaculture pond effluent showed higher BOD, COD value due to the excessive addition of feeds and chemical treatment of ponds.

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In the 3rd day treatment sample the anoxygenic phototrophic bacteria grew well of pH 8.5. The BOD, COD reduced by 23% and 25% respectively. TDS, TSS also reduced by 54.5 and 81% respectively. During the final day treatment TSS,TDS concentration reduced by 92% and 75% respectively. This treatment procedure clearly picturizes the ability of the anoxygenic phototrophic bacteria in effectively degrading the waste present in aquaculture pond effluent.

SUMMARY : 

SUMMARY The fish waste water was collected from Raj Hatchery in kovalam near Chennai, by using sterile containers and was transported to the laboratory immediately. The phototrophic bacterial medium was prepared and the waste water was used as seeding inocula to isolate anoxygenic photosynthetic organisms.

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After 15 days the growth and red colour appeared in culture vessel which was microbiological charecterised. Mass culturing of Rhodopseudomonas spp was done in anaerobic culture vessel which yielded high biomass. The physiochemical parameters of the raw effluent were primarily analysed. The pure cultures of organisms were take and they were seeded into anaerobic bioreactor setup and physiochemical parameters like pH, BOD, COD, TS, TDS, TSS, TN, TP and TOC were analysed every three days interval. The results were recorded, tabulated, analyzed and discussed.

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