sandwich elisa process

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Presentation Description

The slide describe the process of sandwich ELISA technique

Comments

By: vennelatulluri (37 month(s) ago)

i would lyk to have this slide.this is really a gud one

By: SUPERMAN03 (41 month(s) ago)

i WOULD LIKE TO HAVE A COPY OF THIS SLIDE , THIS IS AN AWESOME PPT , GREST WORK

Presentation Transcript

Slide 1:

ELISA (Sandwich) Y Y Y Y Y Well coated with anti-DPD antibodies Incubated overnight at 4 0 C Wash 3x Y Y Y Y Y Diluted plasma added (DPD bind to anti-DPD antibodies) Wash 4x Y Y Y Y Y 0.1 ug of biotinylated anti-human DPD Ig secondary Ab Y Y Wash 4x Y Y Y Y Y Y Y Add 1:2000 dilution of streptavidin conjugated to alkaline phosphatase E E Wash 4x Y Y Y Y Y Y Y E E Alkaline phosphatase substrate is added and developed color read at 405 nm wavelength to measure DPD plasma concentartion Incubated at room temperature 24 0 C 24 h 2 h 2 h 1 h (www. simplebiomol.blogspot.com)