Types of centrifuges : Types of centrifuges PowerPoint Presentation: Depending on the particular application, centrifuges differ in their overall design and size. a common feature in all centrifuges is the central motor that spins a rotor containing the samples to be separated PowerPoint Presentation: Types of Centrifuge depends on : Maximum speed of sedimentation Presence /absence of vacuum Temperature control refrigeration. Volume of sample and capacity of centrifugation tubes PowerPoint Presentation: Small Benchtop with or without refrigeration slow speed ( eg up to 4000 RPM) common in clinical lab (blood/plasma/serum separation) can take approx (up to) 100 tubes, depending on diameter PowerPoint Presentation: 2. Microcentrifuges (“ microfuge ”, “Eppendorf”) take tubes of small vols (up to 2 mL ) very common in biochemistry/molecular biology/ biological labs can generate forces up to ~15,000 x g with or without refrigeration PowerPoint Presentation: 3.High Speed centrifuges 15,000 – 20,000 RPM centrifugal field of 100,000 g large sample capacity depending on rotor normally refrigerated research applications. Differentiation separation of nucleus, mitochondrial, protein precipitate, large intact organelle, cellular debris, bulky protein aggregates. PowerPoint Presentation: 4. Ultracentrifuges 65,000 RPM (100,000’s x g) limited lifetime Expensive require special rotors care in use – balance critical! research applications The high speeds used in such devices generate considerable amounts of heat Therefore cooling arrangements and vaccuum are required in ultracentrifuges 5. Large-capacity preparative centrifuge: 5. Large-capacity preparative centrifuge Centrifugal fields of 3000 to 7000g. Efficient separation of coarse precipitates or whole cells.