Types of centrifuges

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Types of centrifuges :

Types of centrifuges

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Depending on the particular application, centrifuges differ in their overall design and size. a common feature in all centrifuges is the central motor that spins a rotor containing the samples to be separated

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Types of Centrifuge depends on : Maximum speed of sedimentation Presence /absence of vacuum Temperature control refrigeration. Volume of sample and capacity of centrifugation tubes

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Small Benchtop with or without refrigeration slow speed ( eg up to 4000 RPM) common in clinical lab (blood/plasma/serum separation) can take approx (up to) 100 tubes, depending on diameter

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2. Microcentrifuges (“ microfuge ”, “Eppendorf”) take tubes of small vols (up to 2 mL ) very common in biochemistry/molecular biology/ biological labs can generate forces up to ~15,000 x g with or without refrigeration

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3.High Speed centrifuges 15,000 – 20,000 RPM centrifugal field of 100,000 g large sample capacity depending on rotor normally refrigerated research applications. Differentiation separation of nucleus, mitochondrial, protein precipitate, large intact organelle, cellular debris, bulky protein aggregates.

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4. Ultracentrifuges 65,000 RPM (100,000’s x g) limited lifetime Expensive require special rotors care in use – balance critical! research applications The high speeds used in such devices generate considerable amounts of heat Therefore cooling arrangements and vaccuum are required in ultracentrifuges

5. Large-capacity preparative centrifuge:

5. Large-capacity preparative centrifuge Centrifugal fields of 3000 to 7000g. Efficient separation of coarse precipitates or whole cells.