ISOLATION OF BIOLUMINESCENT BACTERIA FOM MARINE ORGANISMS AND

ISOLATION OF BIOLUMINESCENT BACTERIA FrOM MARINE ORGANISMS AND ITS APPLICATION: 

ISOLATION OF BIOLUMINESCENT BACTERIA FrOM MARINE ORGANISMS AND ITS APPLICATION By BAF0052

What is bioluminescence: 

What is bioluminescence Bio-living matter Lumin -to fill with light Escence -process that changes the state The term bioluminescence was coined by Newton Harvey in 1916. Bioluminescence is the conversion of chemical energy into light within the living organisms.

Mechanism of bioluminescent bacteria : 

Mechanism of bioluminescent bacteria Light is produced by most bioluminescent organisms when a organic molecule called luciferin reacts with oxygen to produce light and inactive oxyluciferin . The reaction between luciferin and oxygen is catalyzed by the enzyme luciferase . luciferin+oxygen -- ------------> oxyluciferin+light luciferase Sometimes luciferin and the equivalent protein of luciferase will bound together in a single molecule called ‘’ photoprotein ’’ which can be triggered by calcium ions.

Bioluminescence reaction : 

Bioluminescence reaction

PowerPoint Presentation: 

The bioluminescence reaction is catalyzed by the luciferase enzyme coded by the lux AB genes. Three key substrates modulate the light reaction The dissolved oxygen provided by the surrounding environment. The reduced flavin mononucleotide coming from the bacterial metabolism. A long chain aldehyde coded by the luxCDE genes.

Genetics of bioluminescent bacteria: 

Genetics of bioluminescent bacteria The bacterial luciferin-luciferase system is coded by a set of genes labelled the lux operon . The lux operon is the 9 kilo base pair fragment. Two types of lux operon 1.right lux operon 2.left operon

PowerPoint Presentation: 

The right lux operon consist of 5 genes ( Lux CDABE ) for the emission of visible light. Lux A and Lux B code for the components of luciferase . Lux CDE codes for the fatty acid reductase complex that makes the fatty acids necessary for the luciferase mechanism. Lux R and Lux L involved in regulating the operon .

PowerPoint Presentation: 

The left operon is responsible for the expression of Lux R which encodes for receptor protein for the autoinducer . At the front of the right Lux operon is Lux I,is responsible for synthesizing the autoinducer . Recently another gene has been identified Lux G and it function is mystery. The receptor autoinducer complex bind a specific sequence of DNA in front of right operon and activates the Lux structural genes.

Bioluminescent bacteria: 

Bioluminescent bacteria 1.vibrio fischeri 2.vibrio harveyi 3.vibrio phosphoreum 4.shewanella hanedai 5.shewanella woodyi 6.Dinoflagellates

Collection of fishes : 

Collection of fishes Variety of fishes were collected from the market. Squid and shrimps varieties were separated and smatched .

Isolation of bioluminescent bacteria: 

Isolation of bioluminescent bacteria Placed the smatched squid in the 250ml conical flask. Added 3% NaCl solution. Incubated the flask at room temperature. Observed at interval upto 24 hours.

Media preparation : 

Media preparation luminescent agar The media was prepared and autoclaved. After autoclaving the media was poured in a petriplate . Spread plate technique and pour plate technique was carried out. Kept for incubation at room temperature.

FUNCTIONS OF BIOLUMINESCENT BACTERIA: 

FUNCTIONS OF BIOLUMINESCENT BACTERIA 1.Communication 2.Feeding 3.luminous lure 4.Mating 5.Defense 6.camouflage

THANK YOU by s.shamini : 

THANK YOU by s.shamini