APPLICATIONS OF GC & HPLC

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APPLICATIONS OF HPLC & GC:

APPLICATIONS OF HPLC & GC SUBMITTED BY: SANDHYA RANI NAGABHAIRAVA 11AB1S0418 M.PHARM(PA&QA) GUIDED BY: DR.P.RAVI SHANKAR ASSOCIATE PROFESSOR

Applications of high performance liquid chromatography:

Applications of high performance liquid chromatography

APPLICATIONS OF HPLC::

APPLICATIONS OF HPLC: SEPARATION OF ANALGESICS:

Isocratic elution::

Isocratic elution:

Separation of Polar analgesics: :

Separation of Polar analgesics:

Separation of non-polar analgesics: :

Separation of non-polar analgesics:

GRADIENT ELUTION::

GRADIENT ELUTION: The gradient method operated as follows. When the sample was first loaded onto the column, the mobile phase was predominantly water. Consequently, the more polar Aspirin, Acetaminophen and caffeine traversed the stationary phase. The relatively non-polar Naproxen and Ibuprofen remained tightly adsorbed at the column’s gate. After two minutes, switching to absolute methanol decreased the polarity of the mobile phase. As a result, the non-polar analgesics, Naproxen and Ibuprofen, were successfully dislodged from the non-polar matrix and eluted by the solvent

CORTICOSTEROIDS::

CORTICOSTEROIDS:

PowerPoint Presentation:

S.NO NAME OF THE CORTICO STEROID REMARK 1 PREDNISOLONE ELUTES FIRST BECAUSE IT IS DEVOID OF LIPOPHILIC METHYL GROUP AT C-16 2 BETAMETHASONE ELUTES SECOND DUE TO THE PRESENCE OF F AT C-9 WHICH CONTRIBUTES THE OVERALL LIPOPHILICITY 3 BETAMETHASONE-17-VALERATE VALERATE AT C-17 WITH HUGE ESTER MOIETY MASKS THE –OH MOIETY AT C-11/C-21 4 BETAMETHASONE-21-VALERATE VALERATE AT C-21 MASKS THE –OH MOIETY AT C-17/C-21 5 BETAMETHASONE-17,21-DIPROPIONATE TWO LIPOPHILIC PROPIONATE ESTER MOIETIES AT C-17,C-21 MASKS THE STRONGEST –OH GROUP AT C-11

ALKALOIDS & GLYCOSIDES::

ALKALOIDS & GLYCOSIDES: NATURAL PRODUCTS ACTIVE PLANT CONSTITUENTS APPLICATIONS Alkaloids Morphine, codeine Narcotic analgesics, antitussives glycosides Digitalis glycosides Cardiovascular diseases sennosides laxatives

ANDROGEN DRUGS::

ANDROGEN DRUGS:

ANTI-ARRYTHMATIC DRUGS::

ANTI-ARRYTHMATIC DRUGS:

ANTI-ASTHMATIC DRUGS::

ANTI-ASTHMATIC DRUGS:

ANTI-BACTERIAL DRUGS ::

ANTI-BACTERIAL DRUGS :

PowerPoint Presentation:

ANALYSIS OF TRIMETHOPRIM AND SULPHAMETHOXAZOLE IN CORTIM DIOLAN FORTE CYSTITIS TABLET

ANTI DEPRESSANTS::

ANTI DEPRESSANTS:

ANTI-EPILEPTIC DRUGS::

ANTI-EPILEPTIC DRUGS:

ANTI-OESTROGEN DRUG::

ANTI-OESTROGEN DRUG:

ANTI HISTAMANIC DRUGS: :

ANTI HISTAMANIC DRUGS:

ANTI-HYPERTENSIVE DRUGS::

ANTI-HYPERTENSIVE DRUGS:

PowerPoint Presentation:

INCREASING TEMPERATURE LEADS TO IMPROVED PEAK SHAPE

ANTI-INFLAMMATORY DRUGS::

ANTI-INFLAMMATORY DRUGS:

ANTI-PROTOZOAL DRUGS::

ANTI-PROTOZOAL DRUGS:

ANTI-TUMOR DRUGS::

ANTI-TUMOR DRUGS:

ANTITUSSIVE DRUGS: :

ANTITUSSIVE DRUGS:

CATECHOLAMINES::

CATECHOLAMINES:

H2 -ANTAGONISTS::

H 2 -ANTAGONISTS:

HYPNOTIC DRUGS::

HYPNOTIC DRUGS:

KERATOLYTIC DRUGS::

KERATOLYTIC DRUGS:

MUSCLE-RELAXANTS::

MUSCLE-RELAXANTS:

SEDATIVE DRUGS::

SEDATIVE DRUGS:

SULPHA DRUGS::

SULPHA DRUGS:

THERAPEUTIC PEPTIDES::

THERAPEUTIC PEPTIDES:

TRICYCLIC ANTI-DEPRESSANTS::

TRICYCLIC ANTI-DEPRESSANTS:

FAT-SOLUBLE VITAMINS::

FAT-SOLUBLE VITAMINS:

WATER-SOLUBLE VITAMINS::

WATER-SOLUBLE VITAMINS:

ATROPA BELLADONA EXTRACT::

ATROPA BELLADONA EXTRACT:

CINCHONA CORTEX EXTRACT::

CINCHONA CORTEX EXTRACT:

EPHIDRINE SINICA EXTRACT::

EPHIDRINE SINICA EXTRACT:

GINKO BILOBA EXTRACT::

GINKO BILOBA EXTRACT:

Applications of gas chromatography:

Applications of gas chromatography

HERBICIDES AND PESTICIDES::

HERBICIDES AND PESTICIDES: Analysis in a matter of few minutes and routine drug analysis of few drugs in a matter of few seconds also possible. For example, 2&1/2 min separation of three common pesticides, methyl parathion, malathion , ethion at pg (parts per billion) levels.

PETROLEUM INDUSTRY::

PETROLEUM INDUSTRY: Many compounds can be resolved nicely. Gasoline has been resolved in to over 300 different peaks complex sample of petroleum.

FOOD INDUSTRY::

FOOD INDUSTRY: Employed to account for colour and flavour of food. Determination of residual solvents in spice, oleoresins, for pesticides in foods. Ultra sensitive detectors such as flame ionisation, electron capture are used. These detectors are sensitive to 10 -12 g.per.cc of carrier gas and suiter to sampling environments over crushed fruit, milk, bread. Long capillary and packed columns are used.

BIOCHEMICAL AND CLINICAL FIELDS::

BIOCHEMICAL AND CLINICAL FIELDS: Blood gases, estrogens, howova-nillic acid, vanilimandelic acid, hydroxy corticosteroids can be determined and analysed. Blood alcohol analysis is used by law enforcement to determine if a driver was unlawfully operating a vehicle. Steroids are separated on a silicone rubber gum column at 235 o c.

PHARMACEUTICALS::

PHARMACEUTICALS: Determination of styrene monomer vinyl toulene, latex toulene di-isocyanate in paint, varnish and lacquer and that of formaldehyde and phenol in phenol formaldehyde resins. Non-volatile materials like alkyl resins must be either saponified or pyrolysed for identification purposes.

PLASTIC INDUSTRY::

PLASTIC INDUSTRY: Identification of plastics Determination of esters in acrylic co polymers Determination of long chain alcohol esters in acrylic co polymers Determination of styrene monomers in styrene plastics. Determination of vinyl acetate in its co polymer

BARBITURATE ENANTIOMERS::

BARBITURATE ENANTIOMERS: carrier gas: helium, 20 cm/sec @ 275 °C column: β-DEX 120, 30 m × 0.25 mm I.D., 0.25 μm. det.: FID, 300 °C injection: 100:1 split oven: 210 °C

ALKALOIDS & BARBITURATES::

ALKALOIDS & BARBITURATES:

GC-MS IN THYROID CANCER::

GC-MS IN THYROID CANCER: Has been used to investigate the pathogenic differences in the form of cancer acoording to gender and menopausal conditions. scientists sought to evaluate the metabolic changes in urinary steroids in men and pre- and post-menopausal women with papillary thyroid carcinoma (PTC). GC-MS was used to measure the urinary concentrations of 84 steroids in all of the patients against corresponding controls.

WHISKY SAMPLES::

WHISKY SAMPLES: In quality control of alcoholic beverages it is often important to compare different production batches to detect possible changes in the fermentation and/or distillation process. This note describes the application of a simple and fast GC method with MS detection using the Pegasus II Time of-Flight GC/MS detector.

MISCELLANEOUS PRODUCTS::

MISCELLANEOUS PRODUCTS: A nalysis of: Organic functional groups Gases Coal tar products Fertilizers Alcoholic beverages Air pollutants Natural fats Rubber and rubber products Soap and detergents

conclusion:

conclusion Applications of HPLC and GC are not only limited to the pharmaceutical industry but also widely spread over various industries such as paint industry, petroleum industry These are also used in our routine life for alcohol testing and environmental pollution testing

REFERENCE::

REFERENCE: Pharmaceutical applications with HPLC. A solution guide – Agilent technologies. HPLC- A Practical User’s Guide, 2 nd edition, Marvin C. Mcmaster. Instrumental Methods of Analysis, B.K.Sharma, pg no.C-209 to C-217.

THANK YOU:

THANK YOU

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