logging in or signing up Saponin Glycoside sachinnarkhedein Download Post to : URL : Related Presentations : Let's Connect Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 357 Category: Entertainment License: All Rights Reserved Like it (0) Dislike it (0) Added: March 09, 2013 This Presentation is Public Favorites: 0 Presentation Description Estimation of Saponin Glyucoside Comments Posting comment... By: yatishchandraupa (10 month(s) ago) Sir your presentation is very useful for anyone's knowledge, please allow me to download this. Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript PowerPoint Presentation: 1 Estimation of Saponin Glycoside Sachin Bhaskar Narkhede Smt. BNB Swaminarayan Pharmacy COllege Salvav, Vapi Gujarat IndiaSaponin Glycoside: 2 Saponin Glycoside Quantitative determination: Foam index, haemolytic index and fish index. Fish index: Small fish are put into drug solution of various concentration. The reciprocal of the dilution of the drug necessary to kill 60 % of the fish in the course of 1 hour is called the fish index.Foam index:: 3 Foam index: It is dilution of the substance or a drug to be tested which will give a layer of foam 1 cm high when the aqueous solution is shaken in a graduated cylinder for 15 seconds and then allowed to stand for 15 minutes. VFroth index:: 4 Froth index: 1 g powder drug is macerated with 100 ml H20 for 12 hours. Filter and make the volume of filtrate to 100 ml. Take 10 test-tube of equal diameter (1.6 cm). Add different volume of text into these test tubes [1, 2 .... 10 ml] and adjust the volume up to 10 mlPowerPoint Presentation: 5 in each test tube. Shake each test tube for 15 seconds vigorously and allow them to stand for 15 minutes and observe. Test tube having foam of 1 cm height is noted and concentration of these is taken into consideration.Haemolytic index:: 6 Haemolytic index: It is the concentration of the drug corresponding to 1 g which causes complete haemnolysis of R.B.C. in the blood. Digitonin standard. 1 g of drug with isotonic NaC1 cool for 1 hour.PowerPoint Presentation: 7 The change in absorbance of the supernatant of an erythrocyte suspension is measured after hemolysis by a saponin or a saponin. containing drug. This principle was the basis of the European Pharmacopoeial assay (1st Edition, 1971), which defined the Hemolytic value as 30,000 x a/b,PowerPoint Presentation: 8 with a and b representing the required quantities (in g) of standard and substance being tested, respectively, to obtain complete hemolysis. The value for the standard - a saponin mixture extracted from Gypsophila paniculata L. - is 30,000 by definition. One unit is equal to the quantity (in mL) of bovine blood (l/50 dilution) which is totally hydrolyzed by 1 g of substance. There are two drawbacks to this method: (1) surface-active substances other than saponins can interfere; (2) some saponins are not very or not at all hemolytic (glycyrrhizin). You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.