Marker Assisted Selection :
Marker Assisted Selection Seedlings Genotyping Assays Trait Prediction
What was done :
What was done 4 Parent lines (2 G. Hirsutum, 2 G. barbadense)
Reduced representation libraries
DNA sequencing
Single Nucleotide Polymorphisms (SNPs)
Simple Sequence Repeats (SSRs)
SNP Identification :
SNP Identification Size Selection Genomic Digest Genomic DNA Parent 1 Parent 2 ATGCTGATGATATGCA ATGCTGACGATATGCA Sequence & Identify SNPs Progeny
Sequence Assemblies :
Sequence Assemblies
SNP Discovery :
SNP Discovery
SSR Discovery :
SSR Discovery
Marker Validation :
Marker Validation Gossypium
hirsutum Gossypium
barbadense Maxxa Tx2094 S6 K101 6,469 SNPs 1,679 SNPs
Substitution Frequency :
Substitution Frequency
Genotyping :
Genotyping F2 generation F1 generation Parents 1 1 2 : :
Acknowledgements :
Acknowledgements Dr. Joshua A. Udall
Dr. Peter J. Maughan
Scott Yourstone
Brigham Young University & Plant and Wildlife Department