toxico-pathological effects of intramuscular administration of gentami

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Toxico-Pathological Effects of Intramuscular Administration of Gentamicin in Day-Old Broiler ChicksByMuhammad Kashif Saleemidepts. Veterinary PathologyUniversity of Agriculture FaisalabadPAKISTAN : 

Toxico-Pathological Effects of Intramuscular Administration of Gentamicin in Day-Old Broiler ChicksByMuhammad Kashif Saleemidepts. Veterinary PathologyUniversity of Agriculture FaisalabadPAKISTAN

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INTRODUCTION Gentamicin, an aminoglycoside Antibacterial activity against different isolates from clinical infections of poultry (Giurov, 1985 and 1986, Jonas et al ., 2001) Co-administration with Mereks disease vaccine at dose level of 0.2 mg per chick also reported (Edison et al., 1978)

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Gentamicin unofficially injected into day old chicks to prevent early mortality Gentamicin also administered along with vaccines in Pakistan Unsolved cases of enlarged kidneys and livers in poultry birds suspected for toxicity with unknown causes (Qureshi, 1998)

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Long retention time in body tissues in muscles and livers of turkeys (Shem-Tov et al., 2003) Not allowed in chicken intended for food Used in pet birds and breeding flocks not intended for human consumption

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OBJECTIVES Scanty information about Gentamicin induced toxico-pathological changes associated in avian species prompted to study its induced pathological effects in chicks

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MATERIALS AND METHODS Total of 150 day old broiler chicks (Hubbard Classic) kept on rice husk litter Birds fed corn soybean meal based feed having 22 % total protein Feed and water were provided ad libitum

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Chicks divided in to 15 groups (A-O) having 10 birds in each group All these birds were administered gentamicin (Gentafar®, 10 % gentamicin as gentamicin sulfate, Farvet, Holland) Duration of experiment five weeks

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PARAMETERS STUDIED Clinical signs Water and feed intake Body weight Mortality Gross and microscopic pathology Serum chemistry (Total proteins, Albumin, Creatinine, ALT) LD 50 by probit analysis

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RESULTS Clinical signs and behavioral alterations Birds from groups A-C (0-20 mg/kg) No clinical signs Depression, off feed birds in in Group D (30 mg/kg) 2/10, Groups E &F (40 & 50 mg/kg) 4/10 Group G-J (60-90) More than half of the birds deprssed, profuse diarrhea, sitting on hocks

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Groups K-O (100-180 mg/kg) All birds intensely depressed, diarrhea and off feed, water intake increased

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Mortality

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FEED CONSUMPTION (g/bird/day)

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BODY WEIGHTS OF BROILERS (g)

Serum biochemical parameters of day old broiler administered various levels of gentamicin (Mean ±SD) : 

Serum biochemical parameters of day old broiler administered various levels of gentamicin (Mean ±SD)

PATHOLOGY : 

PATHOLOGY KIDNEYS groups A-E no changes groups F-H moderate swelling, congestion, hemorrhages In groups I-O severe swelling bulged out, congestion, hemorrhages Microscopically all groups had parenchymal congestion necrotic epithelial cells of convoluted tubules

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LIVERS groups A-E no changes Groups F-L moderate enlargement, congestion, friable In Groups M-O Maximum enlargement, friable, hemorrhages on surface

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Groups F-J kidneys showed moderate Liver showed of the birds of groups A, B and D did not exhibit any deviation from the normal histological pattern.

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Microscopically Group E fatty change only Groups F-H fatty change, congestion, of sinosiodal spaces, hetrophilic Aggregates of around triads. Necrotic areas in the parenchyma

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Figure 1: Pyknotic and necrotic changes in glomeruli and tubules of day old broiler chicks (H&E staining) (10X)

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Figure 2: Liver of day old broilers showing hemorrhages and congestion in hepatic triad nuclei of hepatocytes are pyknotic and necrotic changes in hepatocytes (H&E staining) (100X)