logging in or signing up DNA Fingerprinting Forensic rangerblue Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 3125 Category: Education License: All Rights Reserved Like it (3) Dislike it (0) Added: June 14, 2008 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... By: mike2k08 (30 month(s) ago) can you e-mail me this presentation. mike2k08@gmail.com Saving..... Post Reply Close By: rangerblue (30 month(s) ago) I have set it up now so that you may download it know. if this does not work let me know By: rangerblue (30 month(s) ago) I have set it up now so that you may download it know. if this does not work let me know Saving..... Edit Comment Close By: moh_twins (30 month(s) ago) nice Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript DNA Fingerprinting Using PCR : DNA Fingerprinting Using PCR Timothy G. Standish, Ph. D. Polymorphism : Polymorphism Differences between humans can be attributed to two factors: Environmental variation impacting development Individual genes that vary between people These genes which are variable are called polymorphic Each person is genetically unique because of their unique set of polymorphic genes All people are related in that the vast majority of their genes do not vary, but are identical from person to person DNA Fingerprinting : DNA Fingerprinting DNA fingerprinting involves identification of DNA segments which vary between individuals A set of DNA fragments polymorphic enough to provide a unique set of fragments for all individuals, can be used to identify any specific individual in a population No single fragment will uniquely identify an individual, just as no single polymorphic genetic trait will uniquely identify a person, but a unique set of polymorphic DNA traits/fragments can serve as a reliable means of identification Polymorphism In The TPA Gene : Polymorphism In The TPA Gene Tissue Plasminogen Activator (TPA) is a protein that functions in the cascade of reactions which break down blood clots The gene contains 14 exons and 13 introns Scattered within the introns are 28 Alu transposon sequences Within intron 8 a single Alu sequence may be present, or it may be missing Thus, as intron 8 varies with the presence or absence of Alu, it is polymorphic Polymorphism In The TPA Gene : Polymorphism In The TPA Gene PCR Detection of TPA Polymorphism : PCR Detection of TPA Polymorphism PCR will produce a 660 bp fragment 300 base pairs PCR will produce a 660 bp fragment if these primers are used Components of a PCR Reaction : Components of a PCR Reaction Buffer (containing Mg++) Template DNA 2 Primers that flank the fragment of DNA to be amplified dNTPs Taq DNA Polymerase (or another thermally stable DNA polymerase) PCR : PCR PCR : PCR 3’ 5’ Heat PCR : PCR PCR : PCR Heat Heat 5’ PCR : PCR PCR : PCR Heat Heat PCR : PCR PCR : PCR DNA Between The Primers Doubles With Each Thermal Cycle : DNA Between The Primers Doubles With Each Thermal Cycle PCR Detection of TPA Polymorphism : PCR Detection of TPA Polymorphism PCR will produce a 660 bp fragment 300 base pairs PCR will produce a 660 bp fragment if these primers are used Gel Electrophoresis : Gel Electrophoresis You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
DNA Fingerprinting Forensic rangerblue Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 3125 Category: Education License: All Rights Reserved Like it (3) Dislike it (0) Added: June 14, 2008 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... By: mike2k08 (30 month(s) ago) can you e-mail me this presentation. mike2k08@gmail.com Saving..... Post Reply Close By: rangerblue (30 month(s) ago) I have set it up now so that you may download it know. if this does not work let me know By: rangerblue (30 month(s) ago) I have set it up now so that you may download it know. if this does not work let me know Saving..... Edit Comment Close By: moh_twins (30 month(s) ago) nice Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript DNA Fingerprinting Using PCR : DNA Fingerprinting Using PCR Timothy G. Standish, Ph. D. Polymorphism : Polymorphism Differences between humans can be attributed to two factors: Environmental variation impacting development Individual genes that vary between people These genes which are variable are called polymorphic Each person is genetically unique because of their unique set of polymorphic genes All people are related in that the vast majority of their genes do not vary, but are identical from person to person DNA Fingerprinting : DNA Fingerprinting DNA fingerprinting involves identification of DNA segments which vary between individuals A set of DNA fragments polymorphic enough to provide a unique set of fragments for all individuals, can be used to identify any specific individual in a population No single fragment will uniquely identify an individual, just as no single polymorphic genetic trait will uniquely identify a person, but a unique set of polymorphic DNA traits/fragments can serve as a reliable means of identification Polymorphism In The TPA Gene : Polymorphism In The TPA Gene Tissue Plasminogen Activator (TPA) is a protein that functions in the cascade of reactions which break down blood clots The gene contains 14 exons and 13 introns Scattered within the introns are 28 Alu transposon sequences Within intron 8 a single Alu sequence may be present, or it may be missing Thus, as intron 8 varies with the presence or absence of Alu, it is polymorphic Polymorphism In The TPA Gene : Polymorphism In The TPA Gene PCR Detection of TPA Polymorphism : PCR Detection of TPA Polymorphism PCR will produce a 660 bp fragment 300 base pairs PCR will produce a 660 bp fragment if these primers are used Components of a PCR Reaction : Components of a PCR Reaction Buffer (containing Mg++) Template DNA 2 Primers that flank the fragment of DNA to be amplified dNTPs Taq DNA Polymerase (or another thermally stable DNA polymerase) PCR : PCR PCR : PCR 3’ 5’ Heat PCR : PCR PCR : PCR Heat Heat 5’ PCR : PCR PCR : PCR Heat Heat PCR : PCR PCR : PCR DNA Between The Primers Doubles With Each Thermal Cycle : DNA Between The Primers Doubles With Each Thermal Cycle PCR Detection of TPA Polymorphism : PCR Detection of TPA Polymorphism PCR will produce a 660 bp fragment 300 base pairs PCR will produce a 660 bp fragment if these primers are used Gel Electrophoresis : Gel Electrophoresis