HPTLC

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Presentation Description

it describes about principle, instrumentation and applications of high performance thin layer chromatography

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Presentation Transcript

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HPTLC S. PREETHI I M.Sc FSN

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High Performance Thin Layer Chromatography (HPTLC). Recent development led to the improvements in various operations involved. Difference b/w TLC & HPTLC – practical technique than of the physical phenomenon (partition, adsorption) on which separation is based. INTRODUCTION

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INTRODUCTION

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PRINCIPLE Similar approach & same physical properties of TLC. Principle of separation – adsorption. Mobile phase flows through b/c of capillary action. Components move according to their affinities towards adsorbent. Components – separated on chromatographic plate.

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PRINCIPLE

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INSTRUMENTATION

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INSTRUMENTATION

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1. SAMPLE PREPARATION

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2. CHOMATOGRAPHIC LAYERS

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3. SAMPLE APPLICATION

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4. Pre - conditioning Low polarity of mobile phase – no need for saturation. High polarity of mobile phase – saturation is needed. 5. MOBILE PHASE Solution by trial & error where chemical properties of solute & solvent solubility of analytic adsorbent layer are considered.

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Linear development method. plate – vertically. placed in solvent system in a suitable container. solvent – fed by capillary action & chromatogram – from both the sides. 6. CHROMATOGRAPHIC DEVELOPMENT

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7. DETECTION SPOTS Immediately after the development is completed, the plates are removed from the chamber and dried to remove the frees of mobile phase. D etection - known by iodine vapor in iodine chamber.

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8. SCANNING & DOCUMENTATION

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FEATURES

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APPLICATION

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THANK YOU

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