logging in or signing up SEMINAR OF HPLC preciousmolu Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 1920 Category: Entertainment License: All Rights Reserved Like it (2) Dislike it (0) Added: September 16, 2010 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... By: barhates (26 month(s) ago) Hi, i like this presentation please share this ppt to me Saving..... Post Reply Close Saving..... Edit Comment Close By: divyaduth (27 month(s) ago) hi this ppt is useful for me .... can u send it to my mail id plz my mail id is divya2117@gmail.com Saving..... Post Reply Close Saving..... Edit Comment Close By: vaisyarajus (28 month(s) ago) plz send me dis ppt to my mail id plz.......... Saving..... Post Reply Close Saving..... Edit Comment Close By: pharon99 (30 month(s) ago) greaaaaaaaaaaaaaaaaaaaaaat Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript Seminar onHPLC Instrumentation and Solvents Used in HPLC : Seminar onHPLC Instrumentation and Solvents Used in HPLC Guided by: Presented by: Mr. Sumit Bansal Lakhvir Kaur 1 Contents : Contents • Instrumentation. → Solvent Reservoir and Degassing system. → Pumps and Sample injection system. → Column, Detector and Recorder. • Solvent Selection. • Solvent Quality. • References. 2 Instrumentation : Instrumentation Main components of HPLC are: 1. Solvent system and degassing system. 2. Pressure, flow and temperature. 3. Pumps and Sample injection system. 4. Columns and Detectors. 5. Recorder. 3 Instrumentation : Instrumentation 4 Solvent Reservoir and Degassing System : Solvent Reservoir and Degassing System Solvent Reservoir – Convey mobile phase to the degassers. • Degassers – Remove air bubbles or atmospheric gases. 5 Pressure, Flow and Temperature : Pressure, Flow and Temperature Pressure developed in column depends upon: – length of column. – particle size of stationary phase. – viscosity of mobile phase. – flow-rate of mobile phase. • Flow • Temperature 6 Pumps and Sample Injection System : Pumps and Sample Injection System Pumps: Features of HPLC pumps: 1.Solvent flow must be non-pulsing. 2.Interior of pump must not be corroded by any solvent. 3. Easy to dismantle and repair. Types: Constant pressure pump Constant flow pump 7 Pneumatic Pump : Pneumatic Pump 8 Single Piston Reciprocating Pump : Single Piston Reciprocating Pump 9 Dual Piston Reciprocating Pump : Dual Piston Reciprocating Pump 10 Injection System : Injection System Involves the transfer of sample by means of syringe from atm.pressure to a sample chamber. Sample loop valves: -Internal sample loop valve -External sample loop valve - Autosamplers 11 Internal & External Sample Loop Valve : Internal & External Sample Loop Valve 12 Column,Detector and Recorder : Column,Detector and Recorder Columns: - Solvent conditioning column. - Guard column. - Analytical column. • Detector - Refractive index detector. - UV/Visible detector. - Fluorescence detector. -Conductivity detector. • Recorder 13 Solvents : Solvents Solvent selection: Strain(1942) arranged solvents in order of eluting power. It increases with increasing polarity. Solvents: Petroleum ether Carbon tetrachloride Increasing Cyclohexane eluting power Acetone Benzene Alcohols Water 14 Slide 15: Contd…. •In normal phase partition chromatography,stationary phase is more polar than mobile phase. •In reverse phase HPLC,mobile phase is more polar. • Relative polarity depends on the method used to measure it. Greater the adsorption energy,higher the polarity. • Solvent compatibility with detector should be considered. 15 contd….. : contd….. Ranking of solvents by Synder is based on combination of parameters: -Dipole moment -Proton acceptor or donor properties -Dispersion forces 16 Solvent Quality : Solvent Quality Selected solvent should be reasonably constant in physical and chemical properties. Solvents are optimized to give good spectra and have uniform chromatographic properties. Problem with solvents is - Stability - Shelf life 17 contd….. : contd….. Non-polar and intermediate polarity solvents have troublesome properties or preservatives present. Solvents in this category: -Hydrocarbons -Chlorinated solvents -Ethers 18 References : References 1. A.H. Beckett, Stenlake J.B, “Practical Pharmaceutical Chemistry”, 4th Edition, Page no. 90,163-164. 2. Kar A., “Pharmaceutical Drug Analysis”, Page no. 162-165. 3. Munson J.B., ”Modern Pharmaceutical Analysis”, Part-2, Vol-2, Page no.108-110. 4. Chatwal G., “Instrumental Method of Analysis”, 2nd Edition, Page no. 2.625-2.630. 19 THANKYOU : THANKYOU 20 You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
SEMINAR OF HPLC preciousmolu Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 1920 Category: Entertainment License: All Rights Reserved Like it (2) Dislike it (0) Added: September 16, 2010 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... By: barhates (26 month(s) ago) Hi, i like this presentation please share this ppt to me Saving..... Post Reply Close Saving..... Edit Comment Close By: divyaduth (27 month(s) ago) hi this ppt is useful for me .... can u send it to my mail id plz my mail id is divya2117@gmail.com Saving..... Post Reply Close Saving..... Edit Comment Close By: vaisyarajus (28 month(s) ago) plz send me dis ppt to my mail id plz.......... Saving..... Post Reply Close Saving..... Edit Comment Close By: pharon99 (30 month(s) ago) greaaaaaaaaaaaaaaaaaaaaaat Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript Seminar onHPLC Instrumentation and Solvents Used in HPLC : Seminar onHPLC Instrumentation and Solvents Used in HPLC Guided by: Presented by: Mr. Sumit Bansal Lakhvir Kaur 1 Contents : Contents • Instrumentation. → Solvent Reservoir and Degassing system. → Pumps and Sample injection system. → Column, Detector and Recorder. • Solvent Selection. • Solvent Quality. • References. 2 Instrumentation : Instrumentation Main components of HPLC are: 1. Solvent system and degassing system. 2. Pressure, flow and temperature. 3. Pumps and Sample injection system. 4. Columns and Detectors. 5. Recorder. 3 Instrumentation : Instrumentation 4 Solvent Reservoir and Degassing System : Solvent Reservoir and Degassing System Solvent Reservoir – Convey mobile phase to the degassers. • Degassers – Remove air bubbles or atmospheric gases. 5 Pressure, Flow and Temperature : Pressure, Flow and Temperature Pressure developed in column depends upon: – length of column. – particle size of stationary phase. – viscosity of mobile phase. – flow-rate of mobile phase. • Flow • Temperature 6 Pumps and Sample Injection System : Pumps and Sample Injection System Pumps: Features of HPLC pumps: 1.Solvent flow must be non-pulsing. 2.Interior of pump must not be corroded by any solvent. 3. Easy to dismantle and repair. Types: Constant pressure pump Constant flow pump 7 Pneumatic Pump : Pneumatic Pump 8 Single Piston Reciprocating Pump : Single Piston Reciprocating Pump 9 Dual Piston Reciprocating Pump : Dual Piston Reciprocating Pump 10 Injection System : Injection System Involves the transfer of sample by means of syringe from atm.pressure to a sample chamber. Sample loop valves: -Internal sample loop valve -External sample loop valve - Autosamplers 11 Internal & External Sample Loop Valve : Internal & External Sample Loop Valve 12 Column,Detector and Recorder : Column,Detector and Recorder Columns: - Solvent conditioning column. - Guard column. - Analytical column. • Detector - Refractive index detector. - UV/Visible detector. - Fluorescence detector. -Conductivity detector. • Recorder 13 Solvents : Solvents Solvent selection: Strain(1942) arranged solvents in order of eluting power. It increases with increasing polarity. Solvents: Petroleum ether Carbon tetrachloride Increasing Cyclohexane eluting power Acetone Benzene Alcohols Water 14 Slide 15: Contd…. •In normal phase partition chromatography,stationary phase is more polar than mobile phase. •In reverse phase HPLC,mobile phase is more polar. • Relative polarity depends on the method used to measure it. Greater the adsorption energy,higher the polarity. • Solvent compatibility with detector should be considered. 15 contd….. : contd….. Ranking of solvents by Synder is based on combination of parameters: -Dipole moment -Proton acceptor or donor properties -Dispersion forces 16 Solvent Quality : Solvent Quality Selected solvent should be reasonably constant in physical and chemical properties. Solvents are optimized to give good spectra and have uniform chromatographic properties. Problem with solvents is - Stability - Shelf life 17 contd….. : contd….. Non-polar and intermediate polarity solvents have troublesome properties or preservatives present. Solvents in this category: -Hydrocarbons -Chlorinated solvents -Ethers 18 References : References 1. A.H. Beckett, Stenlake J.B, “Practical Pharmaceutical Chemistry”, 4th Edition, Page no. 90,163-164. 2. Kar A., “Pharmaceutical Drug Analysis”, Page no. 162-165. 3. Munson J.B., ”Modern Pharmaceutical Analysis”, Part-2, Vol-2, Page no.108-110. 4. Chatwal G., “Instrumental Method of Analysis”, 2nd Edition, Page no. 2.625-2.630. 19 THANKYOU : THANKYOU 20