logging in or signing up SEMINAR OF HPLC preciousmolu Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 1920 Category: Entertainment License: All Rights Reserved Like it (2) Dislike it (0) Added: September 16, 2010 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... By: barhates (26 month(s) ago) Hi, i like this presentation please share this ppt to me Saving..... Post Reply Close Saving..... 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Pressure, flow and temperature. 3. Pumps and Sample injection system. 4. Columns and Detectors. 5. Recorder. 3 Instrumentation : Instrumentation 4 Solvent Reservoir and Degassing System : Solvent Reservoir and Degassing System Solvent Reservoir – Convey mobile phase to the degassers. • Degassers – Remove air bubbles or atmospheric gases. 5 Pressure, Flow and Temperature : Pressure, Flow and Temperature Pressure developed in column depends upon: – length of column. – particle size of stationary phase. – viscosity of mobile phase. – flow-rate of mobile phase. • Flow • Temperature 6 Pumps and Sample Injection System : Pumps and Sample Injection System Pumps: Features of HPLC pumps: 1.Solvent flow must be non-pulsing. 2.Interior of pump must not be corroded by any solvent. 3. Easy to dismantle and repair. Types: Constant pressure pump Constant flow pump 7 Pneumatic Pump : Pneumatic Pump 8 Single Piston Reciprocating Pump : Single Piston Reciprocating Pump 9 Dual Piston Reciprocating Pump : Dual Piston Reciprocating Pump 10 Injection System : Injection System Involves the transfer of sample by means of syringe from atm.pressure to a sample chamber. Sample loop valves: -Internal sample loop valve -External sample loop valve - Autosamplers 11 Internal & External Sample Loop Valve : Internal & External Sample Loop Valve 12 Column,Detector and Recorder : Column,Detector and Recorder Columns: - Solvent conditioning column. - Guard column. - Analytical column. • Detector - Refractive index detector. - UV/Visible detector. - Fluorescence detector. -Conductivity detector. • Recorder 13 Solvents : Solvents Solvent selection: Strain(1942) arranged solvents in order of eluting power. It increases with increasing polarity. Solvents: Petroleum ether Carbon tetrachloride Increasing Cyclohexane eluting power Acetone Benzene Alcohols Water 14 Slide 15: Contd…. •In normal phase partition chromatography,stationary phase is more polar than mobile phase. •In reverse phase HPLC,mobile phase is more polar. • Relative polarity depends on the method used to measure it. Greater the adsorption energy,higher the polarity. • Solvent compatibility with detector should be considered. 15 contd….. : contd….. Ranking of solvents by Synder is based on combination of parameters: -Dipole moment -Proton acceptor or donor properties -Dispersion forces 16 Solvent Quality : Solvent Quality Selected solvent should be reasonably constant in physical and chemical properties. Solvents are optimized to give good spectra and have uniform chromatographic properties. Problem with solvents is - Stability - Shelf life 17 contd….. : contd….. Non-polar and intermediate polarity solvents have troublesome properties or preservatives present. Solvents in this category: -Hydrocarbons -Chlorinated solvents -Ethers 18 References : References 1. A.H. Beckett, Stenlake J.B, “Practical Pharmaceutical Chemistry”, 4th Edition, Page no. 90,163-164. 2. Kar A., “Pharmaceutical Drug Analysis”, Page no. 162-165. 3. Munson J.B., ”Modern Pharmaceutical Analysis”, Part-2, Vol-2, Page no.108-110. 4. Chatwal G., “Instrumental Method of Analysis”, 2nd Edition, Page no. 2.625-2.630. 19 THANKYOU : THANKYOU 20 You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.