logging in or signing up glass ware nitinkarmore Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 1347 Category: Education License: All Rights Reserved Like it (2) Dislike it (0) Added: October 03, 2008 This Presentation is Public Favorites: 0 Presentation Description all glass ware use in laboratory Comments Posting comment... By: saravanan1977 (18 month(s) ago) the presentation is very nice and is very useful Saving..... Post Reply Close Saving..... Edit Comment Close By: D_Trodden (20 month(s) ago) Would it be possible to get a copy of this presentation? Regards Saving..... Post Reply Close Saving..... Edit Comment Close By: sudandragon (32 month(s) ago) you are the best Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript CARE OF GLASSWARE & PLASTIC WARE : CARE OF GLASSWARE & PLASTIC WARE The striking features of any clinical laboratory is the large display of glassware , plastic ware which are in constant use. IDENTIFICATION AND USE OF COMMON LABORATORY GLASSWARE AND PLASTIC WARE : IDENTIFICATION AND USE OF COMMON LABORATORY GLASSWARE AND PLASTIC WARE Beakers : Beakers Borosilicate science lab glass beakers can be used for routine mixing, measuring and boiling. Similar to proxy beakers in chemical composition, they can be used for all the same basic aqueous measuring, mixing and heating applications where a glass lab beaker is required. Boiling Flasks. : Boiling Flasks. These borosilicate boiling flasks (florence flask) are made to ISO 1773 and are highly resistant to breakage due to heating or chemical attack. A flat bottom boiling flask can be used on a wire mesh, a round bottom one needs a clamp and bosshead. Use instead of an erlenmeyer flask if vigorous boiling is required Burets : Burets Glass burets (burettes) have a variety of applications and are used mainly where accurate amounts of liquid must be added in small amounts. A class B buret will suffice for most non-analytical applications such as acid-base titrations and measuring motor oil for motorcycle racing. Erlenmeyer Flasks : Erlenmeyer Flasks . An erlenmeyer flask is commonly used for simple measuring, storing and mixing of liquids. They can be used as wine or juice containers, culture chambers and brewing vats. Cork and rubber stoppers :To fit them but for many applications, a piece of cellophane (parafilm) will also work. These flasks are autoclavable. We have cycled them repeatedly without any failures. Funnels : Funnels Their are currently two types of funnels. 250, 500 and 1000ml funnels glass with stopcocks and stoppers. The other type is known as a thistle tube or safety funnel. This style is designed to work mainly with larger flasks such as the 1000ml Erlenmeyer flask or filtering flask. Graduated Cylinders : Graduated Cylinders Graduated cylinders are handy for accurate measurements of small volumes of liquid that cannot be done with beakers and flasks. A borosilicate glass graduated cylinder should be used instead of other materials such as polypropylene and TPX if you are going to use oxidizing materials and certain hydrocarbons which can cause plastic to cloud. Pipettes : Pipettes The measuring pipettes are accurate to 0.1% and are ideal for measuring small volumes for doing chemical titrations and serial dilutions for naturopathic medicine tinctures used in homeopathic remedies and other new age therapies. A glass measuring pipette is an autoclavable (sterilizable), reusable, economical alternatives to disposibles. Pipettes : Pipettes Test Tubes : Test Tubes Test tubes are among the most common of requirements for lab supplies. The 18x150mm is considered the standard lab test tube size. The largest, 25x150mm will hold a cigar or spices. A culture tube is a test tube without a lip. Test Tubes : Test Tubes Volumetric Flasks. : Volumetric Flasks. Volumetric flasks are used in analytical chemistry to produce accurate solutions. However, they can be used as decorative glassware around the home for holding salad dressings, etc. Watch glasses. : Watch glasses. Watch glasses have all kinds of uses. They are concave "dishes" that can be used as beaker lids. Reagent Bottles : Reagent Bottles Reagent Bottles - Wide Mouth with Ground Glass Stoppers and many other laboratory products and chemicals for all of your research project needs. Whether work in a lab setting or perform testing in the field, we have the products you need, from Reagent Bottles. Bottle : Bottle Dispensers : Dispensers Made to fit a 38mm buttress, the polyethylene spout has a small air hole that allows finger tip control of pouring speed. Dispensers and many other laboratory products and chemicals for all of your research project needs. Whether work in a lab setting or perform testing in the field, we have the products you need, from Bottle-top Dispensers. Dispensers : Dispensers Cleaning Specific Types of Glassware : Cleaning Specific Types of Glassware Cleaning : Cleaning As is common practice, clean all glassware before use. Any non-abrasive glassware detergent may be used for hand or automatic dishwasher cleaning. If using a dishwasher or glassware dryer, care should be taken to be sure the drying temperature does not exceed 110°C (230°F). Exposure to dry heat should be minimized. Avoid brushes and cleaning pads which could abrade the glass or damage the coating. If using a chromic acid cleaning solution minimize contact of the solution with the coating. Autoclaving : Autoclaving Sterilization time should not exceed 15 minutes at 121°C (250°F). Drying time should not exceed 15 minutes at 110°C (230°F). The actual cavity temperature of the autoclave should be checked to be sure the autoclave temperature does not exceed the recommended sterilization and drying temperature. Autoclaving—Cloudiness: Should the coating appear clouded due to dissolved moisture, simply let dry overnight at room temperature or briefly heat to 110°C (230°F). Burets : Burets Remove the stopcock or rubber tip and wash the buret with detergent and water. Rinse with tap water until all the dirt is removed. Then rinse with distilled water and dry. Wash the stopcock or rubber tip separately. Before a glass stopcock is placed in the buret, lubricate the joint with stopcock lubricant. Use only a small amount of lubricant. Burets should always be covered when not in use. Culture Tubes : Culture Tubes Culture tubes which have been used previously must be sterilized before cleaning. The best method for sterilizing culture tubes is by autoclaving for 30 minutes at 121°C (15 psi pressure). Media which solidifies on cooling should be poured out while the tubes are hot. After the tubes are emptied, brush with detergent and water, rinse with distilled water, place in a basket and dry. If tubes are to be filled with a media which is sterilized by autoclaving, do not plug until the media is added. Both media and tubes are thus sterilized with one autoclaving. If the tubes are to be filled with sterile media, plug and sterilize the tubes in the autoclave or dry air sterilizer before adding the media Dishes and Culture Bottles : Dishes and Culture Bottles Sterilize and clean as detailed under Culture Tubes (above). Wrap in heavy paper or place in a petri dish can. Sterilize in the autoclave or dry air sterilizer. Serological Tubes : Serological Tubes Serological tubes should be chemically clean, but need not be sterile. However, specimens of blood which are to be kept for some time at room temperature should be collected in a sterile container. It may be expedient to sterilize all tubes. Slide 36: To clean and sterilize tubes containing blood, discard the clots in a waste container and place the tubes in a large basket. Put the basket, with others, in a large bucket or boiler. Cover with water, add a fair quantity of soft soap or detergent and boil for 30 minutes. Rinse the tubes, clean with a brush, rinse, and dry with the usual precautions. Slide 37: It is imperative when washing serological glassware that all acids, alkali, and detergents be completely removed. Acids, alkali, and detergents in small amounts interfere with serologic reactions. Serologic tubes and glassware should be kept separate from all other glassware and used only for serologic procedures. Slides and Cover Glass : Slides and Cover Glass It is especially important that microscope slides and cover glass used for the preparation of blood films or bacteriologic smears be perfectly clean and free from scratches. Slides should be washed, placed in glacial acetic acid for 10 minutes, rinsed with distilled water, and wiped dry with clean paper towels or cloth. Once the slides have been washed, place them in a wide jar of alcohol. As needed, remove from the jar and wipe dry. If the slides are dry stored, wash them with alcohol before use Pipettes : Pipettes Place pipettes, tips down, in a cylinder or tall jar of water immediately after use. Do not drop them into the jar. This may break or chip the tips and render the pipettes useless for accurate measurements. A pad of cotton or glass wool at the bottom of the jar will help to prevent breaking of the tips. Be certain that the water level is high enough to immerse the greater portion or all of each pipette. Slide 40: The pipettes may then be drained and placed in a cylinder or jar of dissolved detergent or, if exceptionally dirty, in a jar of chromic acid cleaning solution. After soaking for several hours, or overnight, drain the pipettes and run tap water over and through them until all traces of dirt are removed. Soak the pipettes in distilled water for at least one hour. Remove from the distilled water, rinse, dry the outside with a cloth, shake the water out and dry. Blood Cell Count Diluting Pipettes : Blood Cell Count Diluting Pipettes After use, rinse thoroughly with cool tap water, distilled water, alcohol, or acetone, and then ether. Dry by suction. Do not blow into the pipets as this will cause moisture to condense on the inside of the pipet. General Use and Care Recommendations : General Use and Care Recommendations Proper care and handling of laboratory ware will greatly increase its life and increase the safety of your work place. Do not place laboratory ware over direct heat or open flame. The recommended temperature use range for laboratory ware is 10°C to 80°C. Do not continuously expose laboratory ware to heat above 80°C Slide 43: Do not expose to dry heat in dishwasher above 110°C (230°F). Drying time should not exceed 15 minutes at 110°C (230°F). Do not autoclave above 121°C (250°F). Sterilizing time should not exceed 15 minutes. Do not refrigerate below -20°C (-4°F). Do not remove the protective coating. Do not use a vessel on which the coating is hardened, darkened or otherwise damaged. Slide 44: Do not allow prolonged or repeated exposure of the coating to strong acids or solvents. Do not use a vessel once the glass is broken, immediately transfer the contents of a broken vessel to an approved container and properly dispose of the broken vessel. Do not incinerate discarded vessels. Place in proper disposal containers Slide 45: NITIN D. KARMORE BSc. Medical laboratory Technology 2nd year PRN:07040121018 THANK YOU You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
glass ware nitinkarmore Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 1347 Category: Education License: All Rights Reserved Like it (2) Dislike it (0) Added: October 03, 2008 This Presentation is Public Favorites: 0 Presentation Description all glass ware use in laboratory Comments Posting comment... By: saravanan1977 (18 month(s) ago) the presentation is very nice and is very useful Saving..... Post Reply Close Saving..... Edit Comment Close By: D_Trodden (20 month(s) ago) Would it be possible to get a copy of this presentation? Regards Saving..... Post Reply Close Saving..... Edit Comment Close By: sudandragon (32 month(s) ago) you are the best Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript CARE OF GLASSWARE & PLASTIC WARE : CARE OF GLASSWARE & PLASTIC WARE The striking features of any clinical laboratory is the large display of glassware , plastic ware which are in constant use. IDENTIFICATION AND USE OF COMMON LABORATORY GLASSWARE AND PLASTIC WARE : IDENTIFICATION AND USE OF COMMON LABORATORY GLASSWARE AND PLASTIC WARE Beakers : Beakers Borosilicate science lab glass beakers can be used for routine mixing, measuring and boiling. Similar to proxy beakers in chemical composition, they can be used for all the same basic aqueous measuring, mixing and heating applications where a glass lab beaker is required. Boiling Flasks. : Boiling Flasks. These borosilicate boiling flasks (florence flask) are made to ISO 1773 and are highly resistant to breakage due to heating or chemical attack. A flat bottom boiling flask can be used on a wire mesh, a round bottom one needs a clamp and bosshead. Use instead of an erlenmeyer flask if vigorous boiling is required Burets : Burets Glass burets (burettes) have a variety of applications and are used mainly where accurate amounts of liquid must be added in small amounts. A class B buret will suffice for most non-analytical applications such as acid-base titrations and measuring motor oil for motorcycle racing. Erlenmeyer Flasks : Erlenmeyer Flasks . An erlenmeyer flask is commonly used for simple measuring, storing and mixing of liquids. They can be used as wine or juice containers, culture chambers and brewing vats. Cork and rubber stoppers :To fit them but for many applications, a piece of cellophane (parafilm) will also work. These flasks are autoclavable. We have cycled them repeatedly without any failures. Funnels : Funnels Their are currently two types of funnels. 250, 500 and 1000ml funnels glass with stopcocks and stoppers. The other type is known as a thistle tube or safety funnel. This style is designed to work mainly with larger flasks such as the 1000ml Erlenmeyer flask or filtering flask. Graduated Cylinders : Graduated Cylinders Graduated cylinders are handy for accurate measurements of small volumes of liquid that cannot be done with beakers and flasks. A borosilicate glass graduated cylinder should be used instead of other materials such as polypropylene and TPX if you are going to use oxidizing materials and certain hydrocarbons which can cause plastic to cloud. Pipettes : Pipettes The measuring pipettes are accurate to 0.1% and are ideal for measuring small volumes for doing chemical titrations and serial dilutions for naturopathic medicine tinctures used in homeopathic remedies and other new age therapies. A glass measuring pipette is an autoclavable (sterilizable), reusable, economical alternatives to disposibles. Pipettes : Pipettes Test Tubes : Test Tubes Test tubes are among the most common of requirements for lab supplies. The 18x150mm is considered the standard lab test tube size. The largest, 25x150mm will hold a cigar or spices. A culture tube is a test tube without a lip. Test Tubes : Test Tubes Volumetric Flasks. : Volumetric Flasks. Volumetric flasks are used in analytical chemistry to produce accurate solutions. However, they can be used as decorative glassware around the home for holding salad dressings, etc. Watch glasses. : Watch glasses. Watch glasses have all kinds of uses. They are concave "dishes" that can be used as beaker lids. Reagent Bottles : Reagent Bottles Reagent Bottles - Wide Mouth with Ground Glass Stoppers and many other laboratory products and chemicals for all of your research project needs. Whether work in a lab setting or perform testing in the field, we have the products you need, from Reagent Bottles. Bottle : Bottle Dispensers : Dispensers Made to fit a 38mm buttress, the polyethylene spout has a small air hole that allows finger tip control of pouring speed. Dispensers and many other laboratory products and chemicals for all of your research project needs. Whether work in a lab setting or perform testing in the field, we have the products you need, from Bottle-top Dispensers. Dispensers : Dispensers Cleaning Specific Types of Glassware : Cleaning Specific Types of Glassware Cleaning : Cleaning As is common practice, clean all glassware before use. Any non-abrasive glassware detergent may be used for hand or automatic dishwasher cleaning. If using a dishwasher or glassware dryer, care should be taken to be sure the drying temperature does not exceed 110°C (230°F). Exposure to dry heat should be minimized. Avoid brushes and cleaning pads which could abrade the glass or damage the coating. If using a chromic acid cleaning solution minimize contact of the solution with the coating. Autoclaving : Autoclaving Sterilization time should not exceed 15 minutes at 121°C (250°F). Drying time should not exceed 15 minutes at 110°C (230°F). The actual cavity temperature of the autoclave should be checked to be sure the autoclave temperature does not exceed the recommended sterilization and drying temperature. Autoclaving—Cloudiness: Should the coating appear clouded due to dissolved moisture, simply let dry overnight at room temperature or briefly heat to 110°C (230°F). Burets : Burets Remove the stopcock or rubber tip and wash the buret with detergent and water. Rinse with tap water until all the dirt is removed. Then rinse with distilled water and dry. Wash the stopcock or rubber tip separately. Before a glass stopcock is placed in the buret, lubricate the joint with stopcock lubricant. Use only a small amount of lubricant. Burets should always be covered when not in use. Culture Tubes : Culture Tubes Culture tubes which have been used previously must be sterilized before cleaning. The best method for sterilizing culture tubes is by autoclaving for 30 minutes at 121°C (15 psi pressure). Media which solidifies on cooling should be poured out while the tubes are hot. After the tubes are emptied, brush with detergent and water, rinse with distilled water, place in a basket and dry. If tubes are to be filled with a media which is sterilized by autoclaving, do not plug until the media is added. Both media and tubes are thus sterilized with one autoclaving. If the tubes are to be filled with sterile media, plug and sterilize the tubes in the autoclave or dry air sterilizer before adding the media Dishes and Culture Bottles : Dishes and Culture Bottles Sterilize and clean as detailed under Culture Tubes (above). Wrap in heavy paper or place in a petri dish can. Sterilize in the autoclave or dry air sterilizer. Serological Tubes : Serological Tubes Serological tubes should be chemically clean, but need not be sterile. However, specimens of blood which are to be kept for some time at room temperature should be collected in a sterile container. It may be expedient to sterilize all tubes. Slide 36: To clean and sterilize tubes containing blood, discard the clots in a waste container and place the tubes in a large basket. Put the basket, with others, in a large bucket or boiler. Cover with water, add a fair quantity of soft soap or detergent and boil for 30 minutes. Rinse the tubes, clean with a brush, rinse, and dry with the usual precautions. Slide 37: It is imperative when washing serological glassware that all acids, alkali, and detergents be completely removed. Acids, alkali, and detergents in small amounts interfere with serologic reactions. Serologic tubes and glassware should be kept separate from all other glassware and used only for serologic procedures. Slides and Cover Glass : Slides and Cover Glass It is especially important that microscope slides and cover glass used for the preparation of blood films or bacteriologic smears be perfectly clean and free from scratches. Slides should be washed, placed in glacial acetic acid for 10 minutes, rinsed with distilled water, and wiped dry with clean paper towels or cloth. Once the slides have been washed, place them in a wide jar of alcohol. As needed, remove from the jar and wipe dry. If the slides are dry stored, wash them with alcohol before use Pipettes : Pipettes Place pipettes, tips down, in a cylinder or tall jar of water immediately after use. Do not drop them into the jar. This may break or chip the tips and render the pipettes useless for accurate measurements. A pad of cotton or glass wool at the bottom of the jar will help to prevent breaking of the tips. Be certain that the water level is high enough to immerse the greater portion or all of each pipette. Slide 40: The pipettes may then be drained and placed in a cylinder or jar of dissolved detergent or, if exceptionally dirty, in a jar of chromic acid cleaning solution. After soaking for several hours, or overnight, drain the pipettes and run tap water over and through them until all traces of dirt are removed. Soak the pipettes in distilled water for at least one hour. Remove from the distilled water, rinse, dry the outside with a cloth, shake the water out and dry. Blood Cell Count Diluting Pipettes : Blood Cell Count Diluting Pipettes After use, rinse thoroughly with cool tap water, distilled water, alcohol, or acetone, and then ether. Dry by suction. Do not blow into the pipets as this will cause moisture to condense on the inside of the pipet. General Use and Care Recommendations : General Use and Care Recommendations Proper care and handling of laboratory ware will greatly increase its life and increase the safety of your work place. Do not place laboratory ware over direct heat or open flame. The recommended temperature use range for laboratory ware is 10°C to 80°C. Do not continuously expose laboratory ware to heat above 80°C Slide 43: Do not expose to dry heat in dishwasher above 110°C (230°F). Drying time should not exceed 15 minutes at 110°C (230°F). Do not autoclave above 121°C (250°F). Sterilizing time should not exceed 15 minutes. Do not refrigerate below -20°C (-4°F). Do not remove the protective coating. Do not use a vessel on which the coating is hardened, darkened or otherwise damaged. Slide 44: Do not allow prolonged or repeated exposure of the coating to strong acids or solvents. Do not use a vessel once the glass is broken, immediately transfer the contents of a broken vessel to an approved container and properly dispose of the broken vessel. Do not incinerate discarded vessels. Place in proper disposal containers Slide 45: NITIN D. KARMORE BSc. Medical laboratory Technology 2nd year PRN:07040121018 THANK YOU