Basic Introduction to Paper Chromatography

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simple introduction to paper chromatography, for IGCSE purposes; mainly for Lower 5-level students

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MALVERN COLLEGE QINGDAO LOWER 5 CHEMISTRY MR N. WILSON © Nov 2013

What is paper chromatography?:

What is paper chromatography? Paper chromatography is an analytical method technique for separating and identifying mixtures of organic compounds that are or can be coloured, especially pigments.

[MATERIALS] …what do I need?:

[ MATERIALS ] …what do I need? A B C D E Pencil, ruler, marker with ink

[MATERIALS]:

[ MATERIALS ] Organic solvent [e.g. ethanol] Test tube rack Filter paper (or proper chromatography paper) Test tube with rubber/cork stopper (a slit has been made on the inside part of the stopper) …

[METHOD]…what must I do?:

[ METHOD ]…what must I do? Mark the spot on the paper where you will place the ink Use the dropper and add organic solvent to the tube Secure the paper into the slit made in the stopper, and carefully lower the paper down into the solvent ( MAKE SURE THE SOLVENT DOES NOT REACH ANYWHERE ABOVE YOUR PENCIL MARK ) Use the stopper to close the tube (making it airtight) and wait for about 15 minutes while you complete the worksheet

RESULTS (!!!):

RESULTS (!!!)

ANALYSIS and CONCLUSION:

ANALYSIS and CONCLUSION Some basic questions for analysis : (i) Did everything work as planned? Why or why not? (ii) If you repeat this experiment, will you do everything as you did before or will you change any of the materials or methods? Please explain. (iii) Is there any sense in repeating the experiment? If you feel it should be done, name the reasons why YOU would want to repeat this procedure. Conclusion: This experiment effectively showed that… OR In this experiment we tried to show …. , BUT …

Further Links:

Further Links http://en.wikipedia.org/wiki/Paper_chromatography http://wordnetweb.princeton.edu/perl/webwn?s=paper%20chromatography http://www.chemguide.co.uk/analysis/chromatography/paper.html

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