Blastocyst implantation

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By: frodo786 (68 month(s) ago)

whenever there are two influential people with different ways of dealing things, clashes do occur and either one of them have to leave in the end. They may both be right in their own aspects. And not in all the cases opposites do attract. But Plz we cannot categorize it personality disorder. Do We?

By: frodo786 (68 month(s) ago)

sir all ur presentations are comprehensive and still to the point and help us alot after having read confusing and complicated material from books. Sir we are all gonna miss you plz come back to amdc. You dont need to have to compromise on any matter. Just go with the flow and dont care about anybody.

By: frodo786 (68 month(s) ago)

sir all ur presentations are comprehensive and still to the point and help us alot after having read confusing and complicated material from books. Sir we are all gonna miss you plz come back to amdc. You dont need to have to compromise on any matter. Just go with the flow and dont care about anybody.

Presentation Transcript

Prof. Dr. Saeed Shafi : 

Blastocyst Implantation Prof. Dr. Saeed Shafi

Slide 2: 

Nadia Suleyman, a 32-year old business executive underwent IVF and embryo transfer procedure to combat her primary infertility. The consultant in Fertility Center advised her Gonadotropins 5-days prior to embryo transfer (ET) into the uterine cavity. Moreover, the Embryologist took blood sample to check her hormonal level prior to ET. Explain the embryological reason of using therapy in ART.

Learning Objectives? : 

Learning Objectives? Define Blastocyst Describe the mechanism of its formation Define apoptosis Describe mechanism of implantation

Slide 4: 

Fertilization is a complex sequence of events that begins with contact between a sperm and a secondary oocyte and ends with the intermingling of the maternal and paternal chromosomes at metaphase of 1st cleavage of Zygote.

Oocyte & sperm transport : 

Oocyte & sperm transport Sperm tail motility Vesiculase in seminal secretions form vaginal plug & prevents backflow of semen into vagina. At ovulation , cervical mucous become less viscid. Uterine contractions due to PG in semen Acid pH of vagina decreases sperm motility Alkaline pH of uterus increases sperm motility Chemotaxis by ovum Secondary Oocyte move by Tubal peristalsis

Phases of fertilization : 

contd. Phases of fertilization Passage of sperm through corona radiata Hyaluronidase from acrosome Sperm tail Tubal mucosal enzymes Penetration of zona pellucida facilitated by Acrosin Neurominidase Esterases Zona reaction Lysosomal enzymes of cortical granules

Slide 7: 

contd. Fusion of plasma membranes of secondary oocyte and sperm Completion of 2nd meiotic division of oocyte and formation of female pronucleus Formation of male pronucleus. Ootid Fusion of pronuclei to form Zygote

Slide 8: 

FERTILIZATION

Slide 9: 

The secondary oocyte completes the second meiotic division, forming a mature ovum and the second polar body. The nucleus of the ovum is known as the female pronucleus. Once within the cytoplasm of the ovum the tail of sperm degenerates & its head enlarges to form male pronucleus.

Ootid & Zygote : 

Ootid & Zygote The male and female pronuclei come into contact and loose their nuclear envelopes. The maternal and paternal chromosomes intermingle at equatorial plate of metaphase of the first mitotic division of the zygote.

Slide 11: 

Restoration of diploid chromosomes Species variation Shuffling of genes. The zygote contains a new combination of chromosomes, different from the parents. ALLAH forms the bases of biparental inheritance and results in variation of the human species. Results of Fertilization

Slide 12: 

Cleavage Series of mitotic cell divisions of zygote to form embryonic cells (blastomeres) Implantation Process during which blastocyst attach to endometrium

Slide 13: 

Blastocyst Morula (mulberry) A ball of 12 – 32 blastomeres

SEQUENCE AFTER FERTILIZATION? : 

SEQUENCE AFTER FERTILIZATION? 1st cleavage after 30 Hours Morula 3days Blastocyst formation 4th day Blastocyst floats in Uterus another 2 days ZP ruptures 6th day Blastocyst starts implantation 6th day

HATCHING OF THE BLASTOCYST : 

HATCHING OF THE BLASTOCYST Formation of blastocyst (D4) Shedding of zona pellucida & hatching of the blastocyst (D6) Differentiation of trophoblasts into cytotrophoblasts and syncitiotrophoblasts Formation of hypoblasts from embryoblasts

Slide 16: 

Oocyte Sperm Zygote Fertilization Cleavage Morula Blastocyst Implantation

Slide 17: 

Implantation of Blastocyst

Implantation of Blastocyst : 

Implantation of Blastocyst Day - 4: Morula reaches in uterine cavity Day - 6: Blastocyst touches endometrium Day - 8 &9: Progressive implantation Day - 10: Superficial implantation Day – 12: Complete implantation What is closing plug? When complete regeneration of epithelium of endometrium occurs?

Ectopic Implantation sites? : 

Ectopic Implantation sites? - Placenta previa? IUCD Inhibition of Diethylstibestrol implantation Abortion pills Embryotroph? - hCG?

TAKE HOME MESSAGE : 

TAKE HOME MESSAGE Signs & sumptoms of ovulation (Biphasic temperature) Preselection of embryo and peri-implantation diagnosis of genetic disorders! Sequence of events from Fertilization to implantation! What is cleavage, morula & blastula?