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A Handful of DNA : 

A Handful of DNA This hand contains a lot of DNA but we can’t see it.

Slide 2: 

The more closely we look at the hand, the more details we can see.

Tiny building blocks called cells make up your skin, and every other part of you. Thousands of cells surround every hair growing out of your skin. The cells are in the light purple area at the base of the growing hair. To find the DNA, we have to look more closely at the skin cells. : 

Tiny building blocks called cells make up your skin, and every other part of you. Thousands of cells surround every hair growing out of your skin. The cells are in the light purple area at the base of the growing hair. To find the DNA, we have to look more closely at the skin cells.

Slide 4: 

Different kinds of cells make up different parts of your body. You have fat cells, muscle cells, and skin cells. If you look closely you can see the dark-staining nucleus of these cells. The DNA is smaller still.... skin cells

Slide 5: 

There is DNA in nearly all of your cells, held in a sack called a nucleus. A nucleus is like the brain of the cell. It is where the cell stores all of the information it needs.

Slide 6: 

If you made a little hole in the nucleus sack, you would see DNA peeking through. If you took away the outside of the nucleus, the DNA would spill out like spaghetti.

Deoxyribonucleic acid DNA : 

Deoxyribonucleic acid DNA De oxy ribo nucleic acid An instruction manual for life

DNA has information about your hair: : 

DNA has information about your hair: What colour How long Curly or straight brown curly shiny brown shiny curly

But also…. : 

But also…. Your eye colour Height Shoe size Skin colour Freckles Need glasses Personality traits Who your parents are 1.70m tall blue eyes Size 6 fair Short sighted blonde

DNA contains lots of information about you : 

DNA contains lots of information about you Everyone has information about the same things So everyone’s DNA is different, just like your fingerprints are different from others Scientists have found ways to compare people’s DNA Brown hair Brown skin Short sighted Blonde hair Fair skin Good eye sight

Sir Alec Jeffrey : 

Sir Alec Jeffrey The scientist who invented DNA fingerprinting

Loading a well : 

Loading a well Use a fresh tip 45° angle Side on Just break surface of liquid Gently expel DNA Don’t puncture well!

Prepare to load gel : 

Prepare to load gel Add 5µl Loading dye to each sample Add 10µl size marker (M) DNA to one well Add 20µl DNA to adjacent wells Run 20 minutes 200V

DNA Electrophoresis - Setting up the Apparatus : 

DNA Electrophoresis - Setting up the Apparatus

DNA Electrophoresis - Movement of DNA Fragments : 

DNA Electrophoresis - Movement of DNA Fragments

Tray located on tank platform : 

Tray located on tank platform Wells nearest negative pole

Slide 21: 

Cardiff 3 Man was charged with murdering Cardiff woman 17 years ago.

Slide 23: 

http://pgs-dna.com/

Slide 26: 

Tahini- The Genetic Donor Tabouli and Baba Ganoush The Clones

Stain gel : 

Stain gel Cover gel with Fast Blast DNA stain Leave 2 to 3 minutes De-stain in warm water Agitate gently Leave for 10 to 15 minutes Decide who is the culprit?