logging in or signing up Introduction to Mass Spectroscopy mreisley Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 807 Category: Education License: Some Rights Reserved Like it (1) Dislike it (0) Added: November 01, 2010 This Presentation is Public Favorites: 2 Presentation Description No description available. Comments Posting comment... By: swatiibu (8 month(s) ago) pls send me this presentation this presentation on my email id jmd.swati90@gmail.com and allow me to use this presentation Saving..... Post Reply Close Saving..... Edit Comment Close By: SunnyXav (11 month(s) ago) it is very nice ppt. plz send me on my email id shrutigangwar10@gmail.com Saving..... Post Reply Close Saving..... Edit Comment Close By: drugdiscovery (14 month(s) ago) Hi, please send me this presentation to my email. prof.osama@uni.de. thanks in advance. O. Abd Elrahman Saving..... Post Reply Close Saving..... Edit Comment Close By: mdidris (16 month(s) ago) send me this file to my mail: md_idris3@yahoo.com Saving..... Post Reply Close Saving..... Edit Comment Close By: ramvijay (18 month(s) ago) plz send me this pp Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript Mass Spectroscopy (MS) : Mass Spectroscopy (MS) Determine the relative amounts and masses of: Isotopes Fragments of large molecules Sample is propelled by electrical & magnetic fields Particles separated on the basis of particle mass Peak height indicates relative amount Usually used in conjunction with another instrumental method (FTIR or GC) Mass Spec for IsotopesChlorine : Mass Spec for IsotopesChlorine What do we know? Chlorine is diatomic Chlorine has 2 isotopes Chlorine-35 (~75% abundance) Chlorine-37 (~25% abundance) How many peaks would we get if we tested chlorine? Which peaks would be strongest? 5 35 (atomic Cl) 70 (molecular Cl) Slide 3: A B C D E Identity of Peaks in Chlorine’s Mass Spectrum : Identity of Peaks in Chlorine’s Mass Spectrum A B C D E Monatomic chlorine (35Cl) Monatomic chlorine (37Cl) Molecular (diatomic) chlorine (35Cl- 35Cl) Molecular (diatomic) chlorine (35Cl- 37Cl) Molecular (diatomic) chlorine (37Cl- 37Cl) Interpreting Mass Spectra for Compounds : Interpreting Mass Spectra for Compounds Like a jigsaw puzzle (what fragments/ions could have this mass?) Highest mass peak with highest intensity is probably the non-fragmented molecule (most common isotopes) Both fragments of a “split” should be present Fragmentation commonly occurs at: Branched carbon atoms Mass Spectrum of Methylpropane : Mass Spectrum of Methylpropane A B C Identity of Peaks in Methylpropane’s Mass Spectrum : Identity of Peaks in Methylpropane’s Mass Spectrum A B C Methyl propane Propyl fragment Methyl fragment Interpreting Mass Spectra for Compounds : Interpreting Mass Spectra for Compounds Like a jigsaw puzzle (what fragments/ions could have this mass?) Highest mass peak with highest intensity is probably the non-fragmented molecule (most common isotopes) Both fragments of a “split” should be present Fragmentation commonly occurs at: Branched carbon atoms Carbonyl groups Mass Spectrum of Acetone : Mass Spectrum of Acetone A B C Identity of Peaks in Acetone’s Mass Spectrum : Identity of Peaks in Acetone’s Mass Spectrum A B C Acetone Carbonyl-methyl fragment Methyl fragment Interpreting Mass Spectra for Compounds : Interpreting Mass Spectra for Compounds Like a jigsaw puzzle (what fragments/ions could have this mass?) Highest mass peak with highest intensity is probably the non-fragmented molecule (most common isotopes) Both fragments of a “split” should be present Fragmentation commonly occurs at: Branched carbon atoms Carbonyl groups Look for “characteristic” differences in the peaks Difference of 35 Probably chlorine Difference of 19 Probably fluorine Mass Spectrum of Chloromethane : Mass Spectrum of Chloromethane Difference of 35, which is characteristic of a chlorine atom Interpreting Mass Spectra for Compounds : Interpreting Mass Spectra for Compounds Like a jigsaw puzzle (what fragments/ions could have this mass?) Highest mass peak with highest intensity is probably the non-fragmented molecule (most common isotopes) Both fragments of a “split” should be present Fragmentation commonly occurs at: Branched carbon atoms Carbonyl groups Look for “characteristic” differences in the peaks Difference of 35 Probably chlorine Difference of 19 Probably fluorine If you know possible structure, look for fragments that might match peaks that appear You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
Introduction to Mass Spectroscopy mreisley Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 807 Category: Education License: Some Rights Reserved Like it (1) Dislike it (0) Added: November 01, 2010 This Presentation is Public Favorites: 2 Presentation Description No description available. Comments Posting comment... By: swatiibu (8 month(s) ago) pls send me this presentation this presentation on my email id jmd.swati90@gmail.com and allow me to use this presentation Saving..... Post Reply Close Saving..... Edit Comment Close By: SunnyXav (11 month(s) ago) it is very nice ppt. plz send me on my email id shrutigangwar10@gmail.com Saving..... Post Reply Close Saving..... Edit Comment Close By: drugdiscovery (14 month(s) ago) Hi, please send me this presentation to my email. prof.osama@uni.de. thanks in advance. O. Abd Elrahman Saving..... Post Reply Close Saving..... Edit Comment Close By: mdidris (16 month(s) ago) send me this file to my mail: md_idris3@yahoo.com Saving..... Post Reply Close Saving..... Edit Comment Close By: ramvijay (18 month(s) ago) plz send me this pp Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript Mass Spectroscopy (MS) : Mass Spectroscopy (MS) Determine the relative amounts and masses of: Isotopes Fragments of large molecules Sample is propelled by electrical & magnetic fields Particles separated on the basis of particle mass Peak height indicates relative amount Usually used in conjunction with another instrumental method (FTIR or GC) Mass Spec for IsotopesChlorine : Mass Spec for IsotopesChlorine What do we know? Chlorine is diatomic Chlorine has 2 isotopes Chlorine-35 (~75% abundance) Chlorine-37 (~25% abundance) How many peaks would we get if we tested chlorine? Which peaks would be strongest? 5 35 (atomic Cl) 70 (molecular Cl) Slide 3: A B C D E Identity of Peaks in Chlorine’s Mass Spectrum : Identity of Peaks in Chlorine’s Mass Spectrum A B C D E Monatomic chlorine (35Cl) Monatomic chlorine (37Cl) Molecular (diatomic) chlorine (35Cl- 35Cl) Molecular (diatomic) chlorine (35Cl- 37Cl) Molecular (diatomic) chlorine (37Cl- 37Cl) Interpreting Mass Spectra for Compounds : Interpreting Mass Spectra for Compounds Like a jigsaw puzzle (what fragments/ions could have this mass?) Highest mass peak with highest intensity is probably the non-fragmented molecule (most common isotopes) Both fragments of a “split” should be present Fragmentation commonly occurs at: Branched carbon atoms Mass Spectrum of Methylpropane : Mass Spectrum of Methylpropane A B C Identity of Peaks in Methylpropane’s Mass Spectrum : Identity of Peaks in Methylpropane’s Mass Spectrum A B C Methyl propane Propyl fragment Methyl fragment Interpreting Mass Spectra for Compounds : Interpreting Mass Spectra for Compounds Like a jigsaw puzzle (what fragments/ions could have this mass?) Highest mass peak with highest intensity is probably the non-fragmented molecule (most common isotopes) Both fragments of a “split” should be present Fragmentation commonly occurs at: Branched carbon atoms Carbonyl groups Mass Spectrum of Acetone : Mass Spectrum of Acetone A B C Identity of Peaks in Acetone’s Mass Spectrum : Identity of Peaks in Acetone’s Mass Spectrum A B C Acetone Carbonyl-methyl fragment Methyl fragment Interpreting Mass Spectra for Compounds : Interpreting Mass Spectra for Compounds Like a jigsaw puzzle (what fragments/ions could have this mass?) Highest mass peak with highest intensity is probably the non-fragmented molecule (most common isotopes) Both fragments of a “split” should be present Fragmentation commonly occurs at: Branched carbon atoms Carbonyl groups Look for “characteristic” differences in the peaks Difference of 35 Probably chlorine Difference of 19 Probably fluorine Mass Spectrum of Chloromethane : Mass Spectrum of Chloromethane Difference of 35, which is characteristic of a chlorine atom Interpreting Mass Spectra for Compounds : Interpreting Mass Spectra for Compounds Like a jigsaw puzzle (what fragments/ions could have this mass?) Highest mass peak with highest intensity is probably the non-fragmented molecule (most common isotopes) Both fragments of a “split” should be present Fragmentation commonly occurs at: Branched carbon atoms Carbonyl groups Look for “characteristic” differences in the peaks Difference of 35 Probably chlorine Difference of 19 Probably fluorine If you know possible structure, look for fragments that might match peaks that appear