Biomaterial as Nanomedicine :Biomaterial as Nanomedicine Veerapandian Murugan 2008-40152 Kyungwon university College of Bionanotechnology


Overview :Overview Introduction Present and future status of Nanomedicine Methods Tissue regeneration, drug carrier, diagnostic tool Results and discussion conclusion


Introduction –present status :Introduction –present status Nanotechnology for treatment, diagnosis, monitoring, and control of biological systems-“Nanomedicine” Rational delivery and targeting of pharmaceutical, therapeutic, and diagnostic agents Mononuclear phagocytes, dendritic cells, endothelial cells, and cancers (tumor cells, as well as tumor neo vasculature) are key targets Particle design and formulation


Future Status-Nanomedicine :Future Status-Nanomedicine Detection of single nucleotide polymorphisms, and for gene diagnosis of pathogens Drug discovery, antigens and antibodies Drug infusion, cellular injection-diagnostic procedures Micro chip based drug delivery Nanomechanical sensors, switches and tweezers CNT and nanofibers-drug carriers


Slide 5:Bionanomaterial in tissue regeneration Nanomaterial for bone, cartilage, vascular, bladder, neural applications Tensile strength, hardness, toughness, fracture, Elasticity of modulus, and degree of crystallinity Template for growth at 3D Mimic the properties of host tissue Bioinert, Bioresorbable and Bioactive cartilage tissue engineering process Nature Clinical Practice Rheumatology (2006) 2, 373-382


Methods :Methods Nanofibers as biomaterials Fibers <100nm Interfacial polymerization, Electrospinning, Carbon nanofiber are graphitized catalytic synthesis Application Tissue regeneration- carbon nanofibers + neural stem cells Signal for pathogen identity wound healing nanofibers Filter media, and etc., www.purdue.edu/.../041123.Webster.align.html http://www.sciencemag.org/cgi/content/abstract


Slide 7:Carbon nanotubes Family of fullerenes Tubular form graphite sheets SWNT 0.5–3.0 nm and 20–1000 nm, MWNT 1.5–100 nm and 1–50 µm surface functionalization Molecular sensors, electronic nucleic acid sequencing, and nanoneedles Tris-malonic acid C60-superoxide dismutase- parkinson’s, neuro ischemia, k+ blocker dose dep. www.rsc.org/images/b516309a-300_tcm18-48716.jpg www.wtec.org/loyola/nano/04_03.htm


Slide 8:Polyplexes / Lipopolyplexes Assemblies of nucleic acids b/w polycations/cationic liposome's/ polycations conjugated to targeting ligands or hydrophilic polymers poly-L-lysine, poly (ethylenimine), poly (amidoamine), poly-amino esters, and cationic cyclodextrin. Gene transfer/therapy protocols Transfection protocol www.biochem.arizona.edu/.../Lecture15/AMG7.4.gif


Slide 9:Superparamagnetic iron oxide Crystals Alkaline co-precipitation Fe2+ and Fe3+ with hydrophilic polymer Mag. Moment leads negative enhancer with T1 and T2 effects Surface functionalization of proteins, antibodies, oligonucleotides, Labelling of fluorescein-diagnostic methods www.nsf.gov/od/lpa/news/03/pr0368_images.htm


Slide 10:Liposomes Phospholipid bilayer 100nm(LUVs),Multilamellar vesicles liposome surface - targeting ligands and polymers Drug carrier www.case.edu/.../2002/7-02/liposomes.htm www.unizh.ch/onkwww/lipos.htm


Slide 11:Nanospheres Spherical polymeric matrix 10-100nm Synthetic or natural polymers Good optical, semiconducting Fluorescent and magnetic properties of nanosphere- dignostics like apoptosis and diff. biomedical application www.foodtech-international.com/.../fig1-lge.jpg


Slide 12:Aquasomes (carbohydrate ceramic nanoparticles) Self assembled carrier system-solid phase nanocrystalline core coated with oligomeric film Bodies of water Peptide, protein hormones, antigens and genes to specific sites Microbiology, food chemistry, biophysics and many discoveries www.swri.edu/.../d01/Nano/mages/BonDens3.jpg www.aapsj.org/.../aapsj0902015_figure4.jpg


Results and discussion :Results and discussion Nanoscale laboratory-based diagnostic and drug discovery Micro/nanochip devices, Nanopore sequencing Targeted imaging and drug delivery Anatomical, physiological, immunological or biochemical, and exploitation of opportunities offered by disease states


Conclusion :Conclusion Nanomaterials still at infancy need research Carrier design and targeting strategies must be optimized Toxicity issues Future of Nanomedicine will rationalize to overcome existing biological barriers


Reference :Reference S. Moein Moghimi et.al.,The FASEB Journal • Review H. Liu, T.J. Webster / Biomaterials 28 (2007) 354–369