AFM as a pharmacological tool

Atomic Force Microscopy As A Novel Pharmacological Tool : 

Atomic Force Microscopy As A Novel Pharmacological Tool VEERAPANDIAN MURUGAN 200840152 Kyungwon universtiy College of bionanotechnology

Overview : 

Overview How AFM works Advantages with SEM Methods Results and discussion Conclusion

How AFM works : 

How AFM works Like a profilometer Measures attractive or repulsive forces between tip and sample Detection apparatus measures the vertical deflection for height Can achieve resolution of up to 10 pm Unlike electron microscopes, can measure samples in air and liquid Measuring topography, surface energy, and elasticity of samples at the nanometer, even molecular scale

Advantages with SEM : 

Advantages with SEM Cells are visualized with an uncoated and alive, at high resolution, producing excellent and reproducible images SEM gives a high depth of field and high contrast three dimensio- -nal images of specimen surfaces where cells appear shaded

AFM as a heat detector : 

AFM as a heat detector Immobilization of cantilever Heat of reaction Deflection of cantilever deno- -tes the thermal based trans forming the AFM into an apparatus that could reveal the presence of specific molecules

Pharmacological studies : 

Pharmacological studies Drug interaction investigation Drug immobilization of cantilever and screening of corresponding channel Crystalline α-lactose monohydrate (GranuLac 200,Meggle, Wasser burg, Germany). was used in the form of single particles attached to the cantilever of an AFM or in the form of pure lactose tablets

Identifying defective genes : 

Identifying defective genes Detection of genetic Variations or SNPs by DNA strands are Immobi- -lized on a cantilever surface Nanomechanical property trans- forms the cantilever into a DNA chip Enables study of mutations (Ref) Lee Laboratory Purdue University

Results and discussion : 

Results and discussion Drug delivery by AFM Preformulation Biomolecule interactions and characterization Ligand–receptor interactions Studies of molecule size and crystalline, polymorph properties Formulation Genetic therapy Penicillin

Slide 9: 

Study of anti-virus drugs Clinical analysis Detection of neurotransmitters Fentocalorimeters

References : 

References Ricardo * de Souza Pereira Institute of Biomedical Sciences (ICB), Rua Jean Nassif Mokarzel, 174 Bara˜o Geraldo, Campinas Sa˜o Paulo, Brazil 13084-480 Y.T.A. Turner et al. / Advanced Drug Delivery Reviews 59 (2007) 1453–1473