logging in or signing up Biochip Theory Seminar momugan Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 3020 Category: Science & Tech.. License: All Rights Reserved Like it (1) Dislike it (1) Added: April 15, 2009 This Presentation is Public Favorites: 3 Presentation Description No description available. Comments Posting comment... By: lisaann (4 month(s) ago) give me Saving..... Post Reply Close Saving..... Edit Comment Close By: vershameghwalanil (16 month(s) ago) very good Saving..... Post Reply Close Saving..... Edit Comment Close By: amit610 (19 month(s) ago) its nice Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript MICROFLUIDIC MAGNETIC CELL SORTINGSYSTEM FOR CANCER DIAGNOSIS : MICROFLUIDIC MAGNETIC CELL SORTINGSYSTEM FOR CANCER DIAGNOSIS Biochip Technology Seminar Session Murugan Veerapandian 2008-40152 03/Mar/2009 OUTLINE : OUTLINE Introduction to magnetic microfluidic system for cell sorting and analysis Theory What authors did? Results and discussion Advantages My view on this microfluidic diagnostic strategy INTRODUCTION TO MAGNETIC MICROFLUIDIC SYSTEM FOR CELL SORTING AND ANALYSIS : INTRODUCTION TO MAGNETIC MICROFLUIDIC SYSTEM FOR CELL SORTING AND ANALYSIS Smart system for the diagnosis of rare malignant cells like circulating tumor cells (CTC) Array of magnetic columns coated with antibodies, which has membrane specific protein for target cell capture were taken place inside the chip After trapping in the magnetic columns thier morphology and expression were analysed from microscopy THEORY : THEORY Immunoaffinity separation matrix made by magnetic beads and its self assembly in the column (Magnetic bead flow) Key ref:biomedcentral.inist.fr/images/1477-044X-1-2-1.jpg WHAT AUTHORS DID? : WHAT AUTHORS DID? Detection of B-lymphocytes expressing human protein CD19 membrane protein (CD19+) mixed with T- lymphocytes (CD19-) Cell tracker CMFDA dye The capture of epithelial cells (MCF7 cell line) spiked in a mixture of lymphocytes The beads are grafted either with anti-CD19 (antibody) or anti-EpCAM antibody, depending on the targeted population of cells Cells are observed in bright field and in fluorescent light at high magnification Key ref: NATURE. Vol.450, 20/27, 2007 SCHEMATIC REPRESENTATION OF CELL SEPARATION METHOD AND MICROFLUIDIC CHIP : SCHEMATIC REPRESENTATION OF CELL SEPARATION METHOD AND MICROFLUIDIC CHIP (a) Photography of the microfluidic chip. Magnetic beads are injected in channel 2, the mixture of cells in channel 1 and rinsing buffer in channel 3. The channel 4 is the output of the system. (b) The large section of the separation chamber (50µm in height and 1cm in width) allows a significant throughput. The input channel is divided in a “tree structure” that provide a homogeneous flowdistribution in the separation chamber. (c) Hexagonal arrays of 10 µm diameter ferrofluid spots are formed by microcontact printing. RESULTS AND DISCUSSION : RESULTS AND DISCUSSION In chip immunostaining and May-Grunwald Giemsa coloration of lymphocytes B cells (Raji). (a) Bright field image. (b) Staining with anti-CD19-FITC antibody. (c) Hoechst staining. (d) Merge image of (b) and (c). (e) May-Grünwald-Giemsa staining Cell mixture with 1 positive cell per 1000 negative were studied Small sample size of 0.5 mL/h Magnetic array is directly stamped on a coverslip, which is compatible with modern spectroscopy for high throughput cell sorting and analysis ADVANTAGES : ADVANTAGES Cheaper in fabrication Disposable cell sorting device Requires smaller sample sizes Useful in immunology and cancer research MY VIEW : MY VIEW Evaluation of new class DNA interactive molecule for drug screening application In vitro device for targeted drug deilvery binding study Early diagnosis of autoimmune and neurodegenearaive disorders (A- ß amyloid protofibrils in Alzheimer’s disease, mutant a-synuclein linked early onset of Parkinson’s disease, etc.) REFERENCES : REFERENCES Saias et.al., 552-554, (µTAS2008) Nagrath et al., Nature, 450, 1235 (2007) Doyle et al., Science, 295, 2237 (2002) Fütterer et al., Lab Chip, 4, 351-356 (2004) and www.fluigent.com Slide 11: Thank you for your kind attention Got question? You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
Biochip Theory Seminar momugan Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 3020 Category: Science & Tech.. License: All Rights Reserved Like it (1) Dislike it (1) Added: April 15, 2009 This Presentation is Public Favorites: 3 Presentation Description No description available. Comments Posting comment... By: lisaann (4 month(s) ago) give me Saving..... Post Reply Close Saving..... Edit Comment Close By: vershameghwalanil (16 month(s) ago) very good Saving..... Post Reply Close Saving..... Edit Comment Close By: amit610 (19 month(s) ago) its nice Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript MICROFLUIDIC MAGNETIC CELL SORTINGSYSTEM FOR CANCER DIAGNOSIS : MICROFLUIDIC MAGNETIC CELL SORTINGSYSTEM FOR CANCER DIAGNOSIS Biochip Technology Seminar Session Murugan Veerapandian 2008-40152 03/Mar/2009 OUTLINE : OUTLINE Introduction to magnetic microfluidic system for cell sorting and analysis Theory What authors did? Results and discussion Advantages My view on this microfluidic diagnostic strategy INTRODUCTION TO MAGNETIC MICROFLUIDIC SYSTEM FOR CELL SORTING AND ANALYSIS : INTRODUCTION TO MAGNETIC MICROFLUIDIC SYSTEM FOR CELL SORTING AND ANALYSIS Smart system for the diagnosis of rare malignant cells like circulating tumor cells (CTC) Array of magnetic columns coated with antibodies, which has membrane specific protein for target cell capture were taken place inside the chip After trapping in the magnetic columns thier morphology and expression were analysed from microscopy THEORY : THEORY Immunoaffinity separation matrix made by magnetic beads and its self assembly in the column (Magnetic bead flow) Key ref:biomedcentral.inist.fr/images/1477-044X-1-2-1.jpg WHAT AUTHORS DID? : WHAT AUTHORS DID? Detection of B-lymphocytes expressing human protein CD19 membrane protein (CD19+) mixed with T- lymphocytes (CD19-) Cell tracker CMFDA dye The capture of epithelial cells (MCF7 cell line) spiked in a mixture of lymphocytes The beads are grafted either with anti-CD19 (antibody) or anti-EpCAM antibody, depending on the targeted population of cells Cells are observed in bright field and in fluorescent light at high magnification Key ref: NATURE. Vol.450, 20/27, 2007 SCHEMATIC REPRESENTATION OF CELL SEPARATION METHOD AND MICROFLUIDIC CHIP : SCHEMATIC REPRESENTATION OF CELL SEPARATION METHOD AND MICROFLUIDIC CHIP (a) Photography of the microfluidic chip. Magnetic beads are injected in channel 2, the mixture of cells in channel 1 and rinsing buffer in channel 3. The channel 4 is the output of the system. (b) The large section of the separation chamber (50µm in height and 1cm in width) allows a significant throughput. The input channel is divided in a “tree structure” that provide a homogeneous flowdistribution in the separation chamber. (c) Hexagonal arrays of 10 µm diameter ferrofluid spots are formed by microcontact printing. RESULTS AND DISCUSSION : RESULTS AND DISCUSSION In chip immunostaining and May-Grunwald Giemsa coloration of lymphocytes B cells (Raji). (a) Bright field image. (b) Staining with anti-CD19-FITC antibody. (c) Hoechst staining. (d) Merge image of (b) and (c). (e) May-Grünwald-Giemsa staining Cell mixture with 1 positive cell per 1000 negative were studied Small sample size of 0.5 mL/h Magnetic array is directly stamped on a coverslip, which is compatible with modern spectroscopy for high throughput cell sorting and analysis ADVANTAGES : ADVANTAGES Cheaper in fabrication Disposable cell sorting device Requires smaller sample sizes Useful in immunology and cancer research MY VIEW : MY VIEW Evaluation of new class DNA interactive molecule for drug screening application In vitro device for targeted drug deilvery binding study Early diagnosis of autoimmune and neurodegenearaive disorders (A- ß amyloid protofibrils in Alzheimer’s disease, mutant a-synuclein linked early onset of Parkinson’s disease, etc.) REFERENCES : REFERENCES Saias et.al., 552-554, (µTAS2008) Nagrath et al., Nature, 450, 1235 (2007) Doyle et al., Science, 295, 2237 (2002) Fütterer et al., Lab Chip, 4, 351-356 (2004) and www.fluigent.com Slide 11: Thank you for your kind attention Got question?