logging in or signing up Nanotechnology in vascular tissue engineering mohanamarimuthu Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 4895 Category: Science & Tech.. License: All Rights Reserved Like it (3) Dislike it (0) Added: June 03, 2008 This Presentation is Public Favorites: 0 Presentation Description Upcoming research for vascular tissue engineering Comments Posting comment... By: xandra1 (13 month(s) ago) those who want this ppt, pls sign up in this website and download with in few minutes =) Saving..... Post Reply Close Saving..... 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See all Premium member Presentation Transcript Nanotechnology in vascular tissue engineering: from nanoscaffolding towards rapid vessel biofabrication : Nanotechnology in vascular tissue engineering: from nanoscaffolding towards rapid vessel biofabrication Vladimir Mironov, Vladimir Kasyanov and Roger R. Markwald Bioprinting Research Center, Department of Cell Biology and Anatomy, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA Mohana Marimuthu 200840090 Overview : Overview Introduction Nanotechnology - based control of cell behaviour - in bioengineering of athrombogenicity Nanostructuralized biomimetic vascular scaffolds Magnetic force driven vascular tissue engineering Introduction : Introduction Bioreactor based – expensive, non automated Requirements of vascular graft: Should prevent - thrombosis - vascular intimal thickeneing - aneurysm Approaches - solid scaffold - a hydrogel - cell sheet Slide 4: Nanostructuralized scaffold – mimic ECM Hydrogel – growth factors and ECM peptides – cell and tissue differentiation Nanotechnology – bioreactor free vascular tissue biofabrication Control of cell behaviour : Control of cell behaviour Parameters of Biomaterials ECM ligands/growth factors – cell differentiation, maturation ligand density – cell behaviour – FRET Archeitectures Porous Nanofibres Hydrogels Solid scaffold Cell differentiation and cell behaviour Slide 6: Centrifugal casting technology – solid scaffold + biofabrication of nanopatterned hydrogel Periodic mechanical stretching of cell membranes – magnetic nanoparticles – control cell fate Bioengineering of Athrombogenicity : Bioengineering of Athrombogenicity 3 strategies – immobilization of athrombogenic molecules acellular thromboresistant nanosurfaces – immobilization of molecules – enhance endothelialization – enhancement of endothelialization – iron oxide nanoparticles or magnetic nanobeads – labelled endothelial cells Slide 8: Amphiphilic lipid – like lumenal surfaces – athrombogenic phospholipid polymer Immobilization of CD34 antibodies – post implantational in-vivo endothelialization Nanocomposite bioactive peptides – endothelial monolayer by circulated progenitor cells Nanostructuralized biomimetic vascular scaffolds : Nanostructuralized biomimetic vascular scaffolds Fabrication 1. Phase seperation 2. Electrospinning # nanofibers – size = natural ECM # mimic diameter- collagen fibrils of ECM # macropore structures # Collagen & elastin + synthetic polymers centrifugation/ vacuum - scaffolds rotation technology # Nanofiber meshwork cell attachment Spreading migration # one step fabrication integrated with living cells Slide 11: 3. Self assembly Collagen – structural component ECM – capable of self assembly # cross linking – 3 POG – disulfide # Chemical ligation – POG larger polymers – heterotrimer # heterotrimer – electrostatic interaction - stability Elastin – extensibility # Tropoelastin, elastin like polypeptides, recombinent polypeptides - mechanical property # low productivity, high cost Magnetic force driven vascular tissue engineering : Magnetic force driven vascular tissue engineering Iron oxide nanoparticles – cells – by endocytosis – desirable location Cells labelled with magnetic microbeads – endothelium – driven by magnets Magnets – cell feeding, cell adhesion and monolayer assembly - cell retention Slide 14: Cell sheet technololgy Challenge - undesirable cell activation by nanoparticles - intimal thickening Organ printing technology – robotic biofabrication of intraorgan vessels – vascular spheroids fused with tissues Conclusion : Conclusion Overcome unsolved problems Bioreactor free methods Inexpensive Less time consuming Potential of nanotechnology – cost effective tissue engineered products Slide 19: Thank you You do not have the permission to view this presentation. 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Nanotechnology in vascular tissue engineering mohanamarimuthu Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 4895 Category: Science & Tech.. License: All Rights Reserved Like it (3) Dislike it (0) Added: June 03, 2008 This Presentation is Public Favorites: 0 Presentation Description Upcoming research for vascular tissue engineering Comments Posting comment... By: xandra1 (13 month(s) ago) those who want this ppt, pls sign up in this website and download with in few minutes =) Saving..... Post Reply Close Saving..... Edit Comment Close By: gomber (24 month(s) ago) plz sir i need this presentation urgently so can u sent it to my mail id Saving..... Post Reply Close Saving..... Edit Comment Close By: nehu88 (26 month(s) ago) Hello sir, i hav nanatchnology application inas my seminar topic. sir, can u plzzz send this ppt on my mail it will help me alot...Thank u sir...neha.aswale@gmail.com Saving..... Post Reply Close Saving..... Edit Comment Close By: danish88 (27 month(s) ago) hello sir dis is danish i reely liked the presesntation.can u pleased send this to me on ma mail id ie danish_rock10@yahoo .in. i reely ned it 4 my ppr presentation i must ssay that ur powerpoint skills r reely gud hope u wud rep soon. Saving..... Post Reply Close Saving..... Edit Comment Close By: rsujae (31 month(s) ago) hi sir very infkormative cud you send me the ppt to rsujae@gmail.com. need it for a presentation in college.thank you sir. Saving..... Post Reply Close Saving..... Edit Comment Close loading.... See all Premium member Presentation Transcript Nanotechnology in vascular tissue engineering: from nanoscaffolding towards rapid vessel biofabrication : Nanotechnology in vascular tissue engineering: from nanoscaffolding towards rapid vessel biofabrication Vladimir Mironov, Vladimir Kasyanov and Roger R. Markwald Bioprinting Research Center, Department of Cell Biology and Anatomy, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA Mohana Marimuthu 200840090 Overview : Overview Introduction Nanotechnology - based control of cell behaviour - in bioengineering of athrombogenicity Nanostructuralized biomimetic vascular scaffolds Magnetic force driven vascular tissue engineering Introduction : Introduction Bioreactor based – expensive, non automated Requirements of vascular graft: Should prevent - thrombosis - vascular intimal thickeneing - aneurysm Approaches - solid scaffold - a hydrogel - cell sheet Slide 4: Nanostructuralized scaffold – mimic ECM Hydrogel – growth factors and ECM peptides – cell and tissue differentiation Nanotechnology – bioreactor free vascular tissue biofabrication Control of cell behaviour : Control of cell behaviour Parameters of Biomaterials ECM ligands/growth factors – cell differentiation, maturation ligand density – cell behaviour – FRET Archeitectures Porous Nanofibres Hydrogels Solid scaffold Cell differentiation and cell behaviour Slide 6: Centrifugal casting technology – solid scaffold + biofabrication of nanopatterned hydrogel Periodic mechanical stretching of cell membranes – magnetic nanoparticles – control cell fate Bioengineering of Athrombogenicity : Bioengineering of Athrombogenicity 3 strategies – immobilization of athrombogenic molecules acellular thromboresistant nanosurfaces – immobilization of molecules – enhance endothelialization – enhancement of endothelialization – iron oxide nanoparticles or magnetic nanobeads – labelled endothelial cells Slide 8: Amphiphilic lipid – like lumenal surfaces – athrombogenic phospholipid polymer Immobilization of CD34 antibodies – post implantational in-vivo endothelialization Nanocomposite bioactive peptides – endothelial monolayer by circulated progenitor cells Nanostructuralized biomimetic vascular scaffolds : Nanostructuralized biomimetic vascular scaffolds Fabrication 1. Phase seperation 2. Electrospinning # nanofibers – size = natural ECM # mimic diameter- collagen fibrils of ECM # macropore structures # Collagen & elastin + synthetic polymers centrifugation/ vacuum - scaffolds rotation technology # Nanofiber meshwork cell attachment Spreading migration # one step fabrication integrated with living cells Slide 11: 3. Self assembly Collagen – structural component ECM – capable of self assembly # cross linking – 3 POG – disulfide # Chemical ligation – POG larger polymers – heterotrimer # heterotrimer – electrostatic interaction - stability Elastin – extensibility # Tropoelastin, elastin like polypeptides, recombinent polypeptides - mechanical property # low productivity, high cost Magnetic force driven vascular tissue engineering : Magnetic force driven vascular tissue engineering Iron oxide nanoparticles – cells – by endocytosis – desirable location Cells labelled with magnetic microbeads – endothelium – driven by magnets Magnets – cell feeding, cell adhesion and monolayer assembly - cell retention Slide 14: Cell sheet technololgy Challenge - undesirable cell activation by nanoparticles - intimal thickening Organ printing technology – robotic biofabrication of intraorgan vessels – vascular spheroids fused with tissues Conclusion : Conclusion Overcome unsolved problems Bioreactor free methods Inexpensive Less time consuming Potential of nanotechnology – cost effective tissue engineered products Slide 19: Thank you