Fabrication of biodegradable poly-lactic-glycolic acid (PLGA) scaffold using Bio-MEMS techniques :Fabrication of biodegradable poly-lactic-glycolic acid (PLGA) scaffold using Bio-MEMS techniques Mohana Marimuthu 200840090 College of Bionano Technology Kyungwon University


Overview :Overview Introduction Fabrication of Biodegradable PLGA scaffold soft lithographic technique Microsyringe technique Co2 assisted microfabrication (CAMF) technique Investigation on biodegradable PLGA scaffold Application of PLGA scaffold Conclusion


Introduction :Introduction Tissue engineering – biological substitutes Microenvironment in living tissue - 3 D space Tissue scaffold – reconstruct microenvironment Comparatively polymers – extensively used Several techniques – fabricate scaffolds PLGA scaffold - fabrication, investigation on cell growth and application


Fabrication of Biodegradable PLGA scaffolds :Fabrication of Biodegradable PLGA scaffolds Soft lithographic technique PDMS mold fabrication Micromolding technique Microfluidic technique Spin coating Single layer scaffold 3D scaffold – thermal lamination


2) Microsyringe technique :2) Microsyringe technique


3) Co2 assisted Microfabriction(CAMF) technique :3) Co2 assisted Microfabriction(CAMF) technique 3 steps Photolithography Microembossing Conventional sacrificial layer bilayer Co2 bonding 500 C high pressure syringe pump – 0.69 MPa pressure


Characterization :Characterization Chinese Hamster ovary cells (CHO), Human brain astrocytoma Breast mammary gland tumor cells (MCF-7) Cytocompatibility Cell attachment Cell ingrowth


Investigation on biodegradable PLGA Scaffold :Investigation on biodegradable PLGA Scaffold Attachment and proliferation – human fibroblast cells – PLGA Scaffolds with various pore size – investigated 3 scaffolds – uniform, 2 layer, multilayer pore size HDF cells seeded – 1.5X 105 cells/scaffold Cultured – 2 weeks Multipore size - no. of cells – better cell growth


Application of PLGA scaffold :Application of PLGA scaffold In cartilage regeneration articular cartilage defets – without treatment - osteoarthritis drilling, abrasion, osteochondral grafting and tissue transplantation Have problems like cause pain and change skeletal form PLGA scaffold – tissue engineering – biocompatible, biodegradable, bioactive and provide structural support Successful reconstruction of knees


Slide 16:In Bladder tissue replacement Human bladder smooth muscle cells – seeded Cell growth experiments conducted Enhance cell adhesion and growth - elastin and collagen production Give complex mechanical environment of the bladder walls


Conclusion :Conclusion Control of scaffold architecture – microscale – cell fate and function in tissue engineering Unique platform – cell morphology and tissue development Modify dimensions and porosity – effects on cell attachments,spreading proliferation and differentiation Application – cartilage, bone, bladder tissue, heart muscle and brain tissue regeneration/replacement


Reference :Reference G. Vozzi et al. / Biomaterials 24 (2003) 2533–2540 G. Vozzi et al. / Materials Science and Engineering C 20 (2002) 43–47 Y. Yang et al. / Biomaterials 26 (2005) 2585–2594 J.J. Lee et al. / Current Applied Physics 7S1 (2007) e37–e40 P.X. Ma / Advanced Drug Delivery Reviews 60 (2008) 184–198 K. Uematsu et al. / Biomaterials 26 (2005) 4273–4279 M.A. Pattison et al. / Biomaterials 26 (2005) 2491–2500


Slide 19:Thank you