overview :
overview Introduction
Materials and Methods
Results and Discussion
Conclusion
Introduction :
Introduction Tissue, organ failure – treated by autografts/allografts
Tissue engineering-alternative scaffolds+cells+growth factors
Traditional porous scaffolds – not allow uniform nutrient exchange, limiting cell growth and differentiation.
Soft lithography method – Multilayer Micromolding technique(MMM)
degradable polymers – highly porous polycaprolactone scaffolds
Materials and Methods :
Materials and Methods Scaffold fabrication and characterization
Microfabricaiton of Master by photolithography
Negative su-8 photoresist-5µm
wide,10µm thick ridges, 45µm
spacing
Fabrication of PDMS mold by soft lithography
Scaffold micro fabrication using MMM approach :
Scaffold micro fabrication using MMM approach
Slide 6:
Cell culture experiments
Human Osteosarcoma cells(HOS) seeded
Density of 1X1014 cells/ml
Cultured for 24 and 48 h using Minimum Essential
Medium supplemented with 10 % fetal bovine serum and
1% antibiotics in a humidified incubator
Results and Discussion :
Results and Discussion
Cell culture experiments :
Cell culture experiments
Conclusion :
Conclusion 3 D scaffolds – controlled microfeatures
Lower pressure values - layers
Pore interconnection – facilitate cell ingrowth
Layers – avoid cell clumping Thank you