Multilayer Micromolding of Polymer Scaffolds

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Added: May 21, 2008 This Presentation is Public 
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Multilayer Micromolding Of Degradable Polymer Tissue Engineering Scaffolds :Multilayer Micromolding Of Degradable Polymer Tissue Engineering Scaffolds Daniel Gallego, Nicholas Ferrell, Yang Sun, Derek J. Hansford Biomedical Engineering Department, The Ohio State University, 1080 Carmack Road, 270 Bevis Hall, Columbus (OH), 43210, United States MOHANA MARIMUTHU 200840090


overview :overview Introduction Materials and Methods Results and Discussion Conclusion


Introduction :Introduction Tissue, organ failure – treated by autografts/allografts Tissue engineering-alternative scaffolds+cells+growth factors Traditional porous scaffolds – not allow uniform nutrient exchange, limiting cell growth and differentiation. Soft lithography method – Multilayer Micromolding technique(MMM) degradable polymers – highly porous polycaprolactone scaffolds


Materials and Methods :Materials and Methods Scaffold fabrication and characterization Microfabricaiton of Master by photolithography Negative su-8 photoresist-5µm wide,10µm thick ridges, 45µm spacing Fabrication of PDMS mold by soft lithography


Scaffold micro fabrication using MMM approach :Scaffold micro fabrication using MMM approach


Slide 6:Cell culture experiments Human Osteosarcoma cells(HOS) seeded Density of 1X1014 cells/ml Cultured for 24 and 48 h using Minimum Essential Medium supplemented with 10 % fetal bovine serum and 1% antibiotics in a humidified incubator


Results and Discussion :Results and Discussion


Cell culture experiments :Cell culture experiments


Conclusion :Conclusion 3 D scaffolds – controlled microfeatures Lower pressure values - layers Pore interconnection – facilitate cell ingrowth Layers – avoid cell clumping Thank you