Presentation Transcript
Multilayer Micromolding Of Degradable Polymer Tissue Engineering Scaffolds :Multilayer Micromolding Of Degradable Polymer Tissue Engineering Scaffolds Daniel Gallego, Nicholas Ferrell, Yang Sun, Derek J. Hansford
Biomedical Engineering Department, The Ohio State University, 1080 Carmack Road, 270 Bevis Hall, Columbus (OH), 43210, United States
MOHANA MARIMUTHU
200840090
overview :overview Introduction
Materials and Methods
Results and Discussion
Conclusion
Introduction :Introduction Tissue, organ failure – treated by autografts/allografts
Tissue engineering-alternative scaffolds+cells+growth factors
Traditional porous scaffolds – not allow uniform nutrient exchange, limiting cell growth and differentiation.
Soft lithography method – Multilayer Micromolding technique(MMM)
degradable polymers – highly porous polycaprolactone scaffolds
Materials and Methods :Materials and Methods Scaffold fabrication and characterization
Microfabricaiton of Master by photolithography
Negative su-8 photoresist-5µm
wide,10µm thick ridges, 45µm
spacing
Fabrication of PDMS mold by soft lithography
Scaffold micro fabrication using MMM approach :Scaffold micro fabrication using MMM approach
Slide 6:Cell culture experiments
Human Osteosarcoma cells(HOS) seeded
Density of 1X1014 cells/ml
Cultured for 24 and 48 h using Minimum Essential
Medium supplemented with 10 % fetal bovine serum and
1% antibiotics in a humidified incubator
Results and Discussion :Results and Discussion
Cell culture experiments :Cell culture experiments
Conclusion :Conclusion 3 D scaffolds – controlled microfeatures
Lower pressure values - layers
Pore interconnection – facilitate cell ingrowth
Layers – avoid cell clumping Thank you