logging in or signing up Application of carbor nanotubes in drug delivery mohanamarimuthu Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 3445 Category: Science & Tech.. License: All Rights Reserved Like it (4) Dislike it (0) Added: May 13, 2008 This Presentation is Public Favorites: 0 Presentation Description Nanotubes in drug delivery Comments Posting comment... By: kooltiwari (11 month(s) ago) this give life to many people.so i slute u Saving..... Post Reply Close Saving..... Edit Comment Close By: anju.dm (21 month(s) ago) Its excellent to show the CNTs Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript APPLICATION OF CARBON NANOTUBES IN DRUG DELIVERY : APPLICATION OF CARBON NANOTUBES IN DRUG DELIVERY Alberto Bianco1, Kostas Kostarelos2 and Maurizio Prato3, 1Institute of Molecular and Cellular Biology, UPR 9021 CNRS, 67084 Strasbourg, France2Centre for Drug Delivery Research, The School of Pharmacy, University of London, London WC1N 1AX, UK3Department of Pharmaceutical Sciences, University of Trieste, 34127 Trieste, Italy MOHANA MARIMUTHU PRESENTATION OUTLINE : PRESENTATION OUTLINE Introduction Method Results and discussion -Peptide delivery by CNT -Cellular uptake by CNT -Nucleic acid delivery by CNT -Drug Delivery with CNT Conclusion INTRODUCTION : INTRODUCTION CNT-Vaccine delivery systems or protein transporters CNT synthetic methods- SWNT AND MWNT Pristine CNT – insoluble Soluble CNT – several application Scanning electron microscopy (SEM) images of pristine single- (left) and multi-walled (right) carbon nanotubes. : Scanning electron microscopy (SEM) images of pristine single- (left) and multi-walled (right) carbon nanotubes. METHOD : METHOD 2 funtionalization approaches 1.oxidation 2.Addition reaction 1,3-dipolar cyclo addition of azomethine ylides CNT + α-amino acid + an –CHO DMF f-CNT F-CNT linked to active molecules Slide 6: Figure 2. Organic functionalisation of carbon nanotubes. Pristine single- or multi-walled carbon nanotubes can be (a) treated with acids to purify them and generate carboxylic groups at the terminal parts, or (b) reacted with amino acid derivatives and aldehydes to add solubilising moieties around the external surface. RESULTS AND DISCUSSION : RESULTS AND DISCUSSION PEPTIDE DELIVERY BY CNT B-cell epitope of FMDV + -NH3gp on CNT FMDV peptide-nanotube assessed in vivo in mice high antibody response interact with complement RESULTS AND DISCUSSION : RESULTS AND DISCUSSION II. CELLULAR UPTAKE OF CNT CNT with fluorescent agent epifluorescence and confocal microscopy Passive and endocytosis Protein-CNT in endosomes incubated Hela cells Slide 9: Figure 3. Ultrathin transverse section of HeLa cells treated with functionalized MWNT. After incubation, the cells were fixed stained,dehydrated and embedded into an epoxy resin (Epon® 812). Ultrathin layers (90 nm thickness) were sliced with a diamond ultramicrotome and inspected under TEM. White arrows indicate the f-CNT distributed into the cytoplasm. RESULTS AND DISCUSSION : RESULTS AND DISCUSSION CNT at cell membrane Nanoneedles Nanotube spearing MCF-7 breast cancer cells RESULTS AND DISCUSSION : RESULTS AND DISCUSSION III. NUCLEIC ACID DELIVERY BY CNT Ammonium f-CNT WITH Nucleic acid enhance gene transfer and expression plasmid DNA pCMV- Bgal on –NH3 f-CNT CNT with PEI RESULTS AND DISCUSSION : RESULTS AND DISCUSSION Ammonium CNT condense ODN Immunostimulating activity Mouse splenocytes CNT deliver non-coding RNA polymers into cells Diverse application RESULTS AND DISCUSSION : RESULTS AND DISCUSSION IV. DRUG DELIVERY WITH CNT multiple functionalization of CNT MWNT with amphotericin-B and fluorescein Antifungal activity C.albican, C.parapsilosis, Cryptococcus neoformans Slide 14: Figure 4. Functionalisation and imaging of CNT with amphotericin B. (a) Covalent attachment of amphotericin B and fluorescein isothiocyanate to CNT. Multi-walled carbon nanotubes are treated with acids to generate carboxylic groups, subsequently modified with a mono- protected diaminotriethylene glycol. The nanotubes are then subjected to 1,3-dipolar cycloaddition. Selective cleavage of the orthogonal protecting groups allows the introduction of the fluorescein moiety and amphotericin B. (b) Epifluorescence microscopy image of f-CNT inside Jurkat cells. Bright-field image (left) and fluorescent image (right) after internalisation of f-CNT incubates for 16 h at 37 °C. Jurkat cells have an average diameter of 10 μm. RESULTS AND DISCUSSION : RESULTS AND DISCUSSION SWNT with substituted carborane cages Boron neutron capture therapy Single walled carbon Nanohorns With dexamethasone Experiment in mouse DRUG DELIVERY WITH CNT : DRUG DELIVERY WITH CNT CONCLUSION : CONCLUSION Organic funtionalization Factors -Bio-compatibility -solubility in physiological media high propensity to cross cell membrane interesting and deserve further investigation THANK YOU FOR YOUR PATIENCE : THANK YOU FOR YOUR PATIENCE You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
Application of carbor nanotubes in drug delivery mohanamarimuthu Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 3445 Category: Science & Tech.. License: All Rights Reserved Like it (4) Dislike it (0) Added: May 13, 2008 This Presentation is Public Favorites: 0 Presentation Description Nanotubes in drug delivery Comments Posting comment... By: kooltiwari (11 month(s) ago) this give life to many people.so i slute u Saving..... Post Reply Close Saving..... Edit Comment Close By: anju.dm (21 month(s) ago) Its excellent to show the CNTs Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript APPLICATION OF CARBON NANOTUBES IN DRUG DELIVERY : APPLICATION OF CARBON NANOTUBES IN DRUG DELIVERY Alberto Bianco1, Kostas Kostarelos2 and Maurizio Prato3, 1Institute of Molecular and Cellular Biology, UPR 9021 CNRS, 67084 Strasbourg, France2Centre for Drug Delivery Research, The School of Pharmacy, University of London, London WC1N 1AX, UK3Department of Pharmaceutical Sciences, University of Trieste, 34127 Trieste, Italy MOHANA MARIMUTHU PRESENTATION OUTLINE : PRESENTATION OUTLINE Introduction Method Results and discussion -Peptide delivery by CNT -Cellular uptake by CNT -Nucleic acid delivery by CNT -Drug Delivery with CNT Conclusion INTRODUCTION : INTRODUCTION CNT-Vaccine delivery systems or protein transporters CNT synthetic methods- SWNT AND MWNT Pristine CNT – insoluble Soluble CNT – several application Scanning electron microscopy (SEM) images of pristine single- (left) and multi-walled (right) carbon nanotubes. : Scanning electron microscopy (SEM) images of pristine single- (left) and multi-walled (right) carbon nanotubes. METHOD : METHOD 2 funtionalization approaches 1.oxidation 2.Addition reaction 1,3-dipolar cyclo addition of azomethine ylides CNT + α-amino acid + an –CHO DMF f-CNT F-CNT linked to active molecules Slide 6: Figure 2. Organic functionalisation of carbon nanotubes. Pristine single- or multi-walled carbon nanotubes can be (a) treated with acids to purify them and generate carboxylic groups at the terminal parts, or (b) reacted with amino acid derivatives and aldehydes to add solubilising moieties around the external surface. RESULTS AND DISCUSSION : RESULTS AND DISCUSSION PEPTIDE DELIVERY BY CNT B-cell epitope of FMDV + -NH3gp on CNT FMDV peptide-nanotube assessed in vivo in mice high antibody response interact with complement RESULTS AND DISCUSSION : RESULTS AND DISCUSSION II. CELLULAR UPTAKE OF CNT CNT with fluorescent agent epifluorescence and confocal microscopy Passive and endocytosis Protein-CNT in endosomes incubated Hela cells Slide 9: Figure 3. Ultrathin transverse section of HeLa cells treated with functionalized MWNT. After incubation, the cells were fixed stained,dehydrated and embedded into an epoxy resin (Epon® 812). Ultrathin layers (90 nm thickness) were sliced with a diamond ultramicrotome and inspected under TEM. White arrows indicate the f-CNT distributed into the cytoplasm. RESULTS AND DISCUSSION : RESULTS AND DISCUSSION CNT at cell membrane Nanoneedles Nanotube spearing MCF-7 breast cancer cells RESULTS AND DISCUSSION : RESULTS AND DISCUSSION III. NUCLEIC ACID DELIVERY BY CNT Ammonium f-CNT WITH Nucleic acid enhance gene transfer and expression plasmid DNA pCMV- Bgal on –NH3 f-CNT CNT with PEI RESULTS AND DISCUSSION : RESULTS AND DISCUSSION Ammonium CNT condense ODN Immunostimulating activity Mouse splenocytes CNT deliver non-coding RNA polymers into cells Diverse application RESULTS AND DISCUSSION : RESULTS AND DISCUSSION IV. DRUG DELIVERY WITH CNT multiple functionalization of CNT MWNT with amphotericin-B and fluorescein Antifungal activity C.albican, C.parapsilosis, Cryptococcus neoformans Slide 14: Figure 4. Functionalisation and imaging of CNT with amphotericin B. (a) Covalent attachment of amphotericin B and fluorescein isothiocyanate to CNT. Multi-walled carbon nanotubes are treated with acids to generate carboxylic groups, subsequently modified with a mono- protected diaminotriethylene glycol. The nanotubes are then subjected to 1,3-dipolar cycloaddition. Selective cleavage of the orthogonal protecting groups allows the introduction of the fluorescein moiety and amphotericin B. (b) Epifluorescence microscopy image of f-CNT inside Jurkat cells. Bright-field image (left) and fluorescent image (right) after internalisation of f-CNT incubates for 16 h at 37 °C. Jurkat cells have an average diameter of 10 μm. RESULTS AND DISCUSSION : RESULTS AND DISCUSSION SWNT with substituted carborane cages Boron neutron capture therapy Single walled carbon Nanohorns With dexamethasone Experiment in mouse DRUG DELIVERY WITH CNT : DRUG DELIVERY WITH CNT CONCLUSION : CONCLUSION Organic funtionalization Factors -Bio-compatibility -solubility in physiological media high propensity to cross cell membrane interesting and deserve further investigation THANK YOU FOR YOUR PATIENCE : THANK YOU FOR YOUR PATIENCE