Silver Nanoparticles synthesis and applications

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Bacterial synthesis of silver nano particles and their environmental applications

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Silver Nanoparticles: Bacterial synthesis and their environmental applications:

Silver Nanoparticles : Bacterial synthesis and their environmental applications Manju.Phadke , Jewal Patel Department of Microbiology, SIES College of Arts, Science and Commerce, Sion [W], Mumbai-22, India

Nanoparticle:

Nanoparticle

Why Silver Nanoparticles??:

Why Silver Nanoparticles ??

Methods of Synthesis:

Methods of Synthesis

Materials and Methods:

Materials and Methods

Production of AgNP’S:

Production of AgNP’S

Optimisation Parameters:

Optimisation Parameters

Characterization of AgNPs :

Characterization of AgNPs UV-Visible spectroscopy and Diffraction Light Scattering (DLS)

Environmental Applications of AgNP’S:

Environmental Applications of AgNP’S

Results:

Results

Production of silver nanoparticles :

Production of silver nanoparticles During the production period of 4 days, the colour change from pale yellow to brown indicates reduction of metallic silver.

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Optimum concentration: 2mM and 5mM AgNO 3 Optimum time : 24 hours Effect of Concentration and Time

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: Effect of Temperature

Comparative study of Biologically synthesised and chemically synthesised AgNP’s:

Comparative study of Biologically synthesised and chemically synthesised AgNP’s AgNP’s E.coli Pseudomonas MDR MRSA Biologically Synthesised 17mm 20.5mm 17mm 16.5mm Chemically Synthesised No zone No zone No zone No zone

Antibacterial activity:

Antibacterial activity

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Test organisms AgNP solution prepared using 2 mM AgNO3 AgNP solution prepared using 5 mM AgNO3 Escherichia coli 12.7 mm 17mm Klebsiella pneumoniae 12mm 16mm Corynebacterium diphtheriae 15mm 19.7mm Salmonella typhi 11mm 20mm Pseudomonas aeruginosa 14.7mm 18.4mm Staphylococcus aureus 21mm 23.7mm

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iii) Antimycotic activity: Test organism AgNP solution prepared using 2 mM AgNO3 AgNP solution prepared using 5 mM AgNO3 Aspergillus niger 6.5mm 12.7mm 5 mM 2 mM control Test organism AgNP solution prepared using 2 mM AgNO3 AgNP solution prepared using 5 mM AgNO3 Candida albicans 7mm 15mm

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Key : ̶ : no growth, + : growth, ++ : moderate growth, +++ : luxuriant growth Test Days Control Medium + 5 ml of AgNPS prepared using 2 mM AgNo 3 Medium + 10 ml of AgNPS prepared using 2 mM AgNo 3 Medium + 5ml of AgNPS prepared using 5 mM AgNo 3 Medium + 10 ml of AgNPS prepared using 5 mM AgNo 3 2 ̶ ̶ ̶ ̶ ̶ 4 + ̶ ̶ ̶ ̶ 6 + ̶ ̶ ̶ ̶ 8 ++ ̶ ̶ ̶ ̶ 10 ++ + ̶ ̶ ̶ 12 +++ + ̶ ̶ ̶ 15 +++ ++ ̶ ̶̶ ̶ Control- 0 day Control-15 th day Test - 0 day Test- 15 th day

Coating of membrane filters with AgNP’S:

Coating of membrane filters with AgNP’S

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Fabrics treated with AgNPs solution prepared using AgNO3 of: zone of inhibiton (mm) S. aureus P.aeruginosa 2 mM 10 mins 31mm 32mm 20 mins 33mm 33mm 40 mins 35mm 35mm 5 mM 10 mins 32mm 33mm 20 mins 35mm 36mm 40 mins 37mm 39mm Uncoated cotton fabrics ̶ ̶

Conclusion:

Conclusion

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Authors are grateful to : Dr. Rajedeep Bandopadhyay (IIT Powai ) Ms. Poonam Londhe Mr. Shabbir Bohra ( Sies College, Mumbai)

REFERENCES::

REFERENCES: Duran N, Marcato PD, Alves OL, Souza G, Elisa Esposito E. 2005. Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. Journal of Nanobiotechnology. 86 :1477-3155 Heidarpour F, Ghani K, Razi F, Sobri S, Torabiana A, Heydarpourb V , Zargar M. 2011. New Trends on Microbiological Water Treatment. Digest Journal of Nanomaterials and Biostructures 6 (2): 791 – 802 Kannan N, Subbalaxmi S. 2011. Green Synthesis of Silver Nanoparticles using Bacillus subtillus IA751 and its Antimicrobial activity. Research Journal of Nanoscience and Nanotechnology 1(2) : 87-94 Panacek A, Kola M, Vecerova R, Prucek R, Soukupovaa J, Krystof V, Hamal P, Zboril R , Kvıtek L. 2009. Antifungal activity of silver nanoparticles against Candida spp. Biomaterials 30 : 6333–6340 Saifuddin N, Wong CW, Yasumira AAN. 2008. Rapid Biosynthesis of Silver Nanoparticles Using Culture Supernatant of Bacteria with Microwave Irradiation. E-Journal of Chemistry. 6 (1): 61-70 Sastry M, Ahmad A, Khan MI, Kumar R. 2003. Biosynthesis of metal nanoparticles using fungi and actinomycete Current Science. 85 (2). 162-170 Sharma V K, Yngard R A, Lin Y. 2008. Silver nanoparticles: Green synthesis and their antimicrobial activities. Advances in Colloid and Interface Science 145 : 83-96 Vaidyanathan R, Kalishwaralal K, Gopalram S, Gurunathan S. 2009. Nanosilver—The burgeoning therapeutic molecule and its green synthesis. Biotechnology Advances 27: 924–937

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THANK YOU