KERATOPLASTY

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KERATOPLASTY : 

KERATOPLASTY P. MAHALINGAM BVM 06066

LAYERS OF CORNEA : 

LAYERS OF CORNEA Anterior epithelial layer Bowman’s membrane Corneal stroma Descemet’s membrane Enothelial layer

INDICATIONS : 

INDICATIONS Corneal endothelial dystrophy & degeneration Opaque central corneal scars & blindness Deep corneal ulcers Graft rejection may occur –(post operative vascularization, edema,inflammation,eventual opacification of transplanted cornea)

CORNEAL EDEMA : 

CORNEAL EDEMA

CORNEAL ULCER : 

CORNEAL ULCER

SOURCES OF CORNEAL GRAFTS : 

SOURCES OF CORNEAL GRAFTS Heterologous (from other spp) Homologous(from the same spp) Autlogous(from the same patient, usually a rotating or sliding graft)

TYPES OF KERATOPLASTY : 

TYPES OF KERATOPLASTY Full thickness or penetrating keratoplasty Lamellar keratoplasty (corneal epithelium& anterior stroma)

INSTRUMENTATIONS : 

INSTRUMENTATIONS Eyelid speculum(barraquer wire speculum) Flierringa rings(stabilize peripheral cornea,anterior sclera & anterior segments) Tissue fixation forceps Tenotomy or utility scissors Rt &lt handle corneal trasplantation scissors Microsurgical needle holders

cont………… : 

cont………… Corneal trephines(8-12mm dia in 0.5 increments) Teflon corneal graft block

RECOMMENDED SUTURES : 

RECOMMENDED SUTURES 4-0 to 6-0 braided sutures-temporarily attach the flieringa ring to limbus or sclera 7-0 to 8-0 nylon sutures –graft stabilization (8-16 simple interrupted sutures) 8-0 to 10-0 –simple continuous suture to supplement the interrupted sutures)

PREPARATION OF DONOR CORNEA : 

PREPARATION OF DONOR CORNEA DONOR – should be free of infectious diseases Aseptically remove the globe – several drpos of neomycin,bacitracin,polymyxin-B Placed in sterile moist chamber-cornea upward(use within 7 days) Remove only cornea with 2mm of scleral rim(-20 degree – 18 months)

cont………. : 

cont………. Prior to use epithelial & endothelial with descemet”s membrane surfaces are vigourously scraped leaving corneal stromal collagenous lamellae.

PENETRATING KERTOPLASTY : 

PENETRATING KERTOPLASTY DEFINITION Affeted tissue removed full thickness(all layers of cornea) Replaced with donor button liner with viable endothelium

TECHNIQUE : 

TECHNIQUE Lesion is removed with trephine set at 1mm depth. Trephine is withdrawn immediately on entering the anterior chamber Tissue tags trimmed from the incision edges with corneal scissors & fine forceps Donor button is washed with antibiotics

cont…….. : 

cont…….. Cornea,5mm rim of sclera removed, epithelium is placed on teflon block & donor button is cut from the endothelial side with trephine Donor button is 0.5mm larger in dia than recipient bed to allow for shrinkage(endothelium must not be touched)

cont………. : 

cont………. Donor button is removed & placed in the recipient bed with four cardinal sutures, one in each quadrant.Put interrupted or continuous sutures.After that cardinal sutures are removed. Anterior chamber then reconstituted with balanced salt solution & an air bubble

POST OPERATIVE CARE : 

POST OPERATIVE CARE Topical antibiotics & corticosteroids- four times a day given - postoperatively Postoperative vascularization minimized by fine sutures(8-0 to 10-0 nylon) & corticosteroids(0.1 percent dexamethasone) Sutures removed after 12-18 days.

cont,……… : 

cont,……… Hyaluronic acid & methycellulose in the anterior chamber & on the edges of corneal incision protects the corneal endothelium from surgical trauma. The material is irrigated from the eye after suturing.

cont,…… : 

cont,…… Major problem -formation of protein rich plasmoid aqueous -shrinkage of the donor button -postoperative vascularization

To minimize the formation of plasmoid aqueous : 

To minimize the formation of plasmoid aqueous -Systemic corticosteroids(oral prednisolone 1-2mg/kg daily for 3 days -Topical corticosteroids (dexamethasone 0.1 percent QID several days before surgery) -Topical antiprostaglandins (flurbiprofen or indomethacin) -Systemic antiprostaglandins (flunixin meglumine-1mg/kg I/v at induction -Acetyl salicylic acid-25mg/kg PO daily for 2 days before surgery) -Heparin & epinephrine (itraocular flushing solution)

LAMELLAR KERATOPLASTY : 

LAMELLAR KERATOPLASTY DEFINITION Epithelium & superficial stroma are dissected free & replaced with donor tissue.

TECHNIQUE : 

TECHNIQUE The area to be removed is outlined with a trephine set at 0.3mm Lesion removed with a corneal dissector & number 64 Beaver blade or scissors Donor eye – washed with antibiotic solution.The lamellar graft is removed from the donor eye with an electrokeratotome or a Martinez corneal dissector.

cont……. : 

cont……. The graft is made 0.5mm larger than the recipient bed. The graft is sutured into the recipient bed with either a continuous suture with a burier knot or multiple interrupted sutures. Postoperatively topical antibiotics & corticosteroids are applied 4 times daily.

AUTOGENOUS LAMELLAR CORNEAL TRASPLANTATION : 

AUTOGENOUS LAMELLAR CORNEAL TRASPLANTATION A square block of tissue is excised at the ulcer site for debridement & graft site preparation. A slightly larger graft is harvested by superficial keratectomy-on the same cornea or contralateral cornea. The graft is sutured into the recipient bed. It prevents the graft rejection, but vascularization of the site often occurs

PENETRATING CORNEOSCLERAL ALLOGRAFT : 

PENETRATING CORNEOSCLERAL ALLOGRAFT Replacement of continuous cornea & sclera from adonor of the same species – treatment for canine epibulbar melanomas. Corneoscleral donor tissue is collected – as soon as possible before resection of the lesion & is kept on a sterile gauze pad moistened with balanced salt solution.

cont……… : 

cont……… All traces of uveal tissues are removed from the graft to reduce the severity of postoperative immune reactions. The graft is sutured with 8-0 to 10-0 monofilament nylon. Eye is then treated with topical antibiotics & corticosteroids 4-6 times daily for 7-10 days.

Slide 42: 

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