BIOREACTORS

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Bioreactors: An Artificial Life Support System:

Bioreactors: An Artificial Life Support System Presented by: MITESH SHRESTHA

Contents:

Contents Introduction to Bioreactors Types of Bioreactor designs Conclusion

Bioreactors:

Bioreactors Any manufactured or engineered device or system that supports a biologically active environment

Types of Bioreactor designs:

Types of Bioreactor designs Stirred tank reactors Bubble-column reactors Air lift reactors Drum rotating reactors Immobilized plane cell reactors Membrane reactors

Stirred tank reactors:

Stirred tank reactors Air is dispersed by mechanical agitation.

Advantages:

Advantages Better control over the environment of the culture.

Disadvantages:

Disadvantages Can cause damage to the cells High energy demand Complexity in construction Difficult to scale up.

Bubble-column reactors:

Bubble-column reactors One of the simplest type of gas – liquid bioreactors.

Advantages:

Advantages Facilitates sterile operation Less damaging to shear-sensitive cells Scale up is relatively easy.

Disadvantages:

Disadvantages Undefined fluid flow pattern inside the reactor. Non-uniform mixing.

Air-lift reactors:

Air-lift reactors Works on draught tube principle.

Advantages:

Advantages Reasonable mixing with low shear Operating cost is low. Less contamination

Disadvantages:

Disadvantages Insufficient mixing at high cell densities .

Drum rotating reactors:

Drum rotating reactors Consists of horizontally rotating-drum on rollers connected to a motor.

Advantages:

Advantages High oxygen transfer. Good mixing Facilitated better growth and impart less hydrodynamic stress.

Disadvantages:

Disadvantages Difficult to scale up.

Immobilized plane cell reactors:

Immobilized plane cell reactors Immobilization of plant cell into a suitable carriers. Either in natural (alginate, agar) or synthetic (polyacrylamide)

Membrane reactors:

Membrane reactors Cells are separated from growth medium by membrane

Advantages:

Advantages Environment is more easily controlled Better control over cell density.

PowerPoint Presentation:

Reactor type Oxygen transfer Hydrodynamic stress Mixing Scale up limitations Stirred-tank High Highly destructive Completely uniform Difficult Cell death; contamination due to moving parts ST-low agitation and modified impeller Medium Low Reasonably uniform Difficult Insufficient mixing at very high cell densities Bubble-column Medium Low Non-uniform Easy Dead zones; settling of cells due to poor mixing Air-lift High Low Uniform Easy Dead zones at high cell densities Rotating-drum High Low Uniform Difficult Non-uniform mixing at very large scale.

Conclusion:

Conclusion Hence, with the help of different types of bioreactors, commercial production of secondary metabolites is not only possible but also profitable.

Thank you !:

Thank you !

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