XLD Agar - Manufcture By TM Media

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TM Media, a best Microbiology Culture Media products Manufacturing Company in India. Offering all kind of Culture Media and Biological Media Includes: 1. xld agar 2. sabouraud dextrose agar 3. culture media 4. macconkey agar 5. cled agar 6. mueller hinton agar TM Media is a one stop solution for fulfilling Microbiology Needs, and having wide segment of Clinical Microbiology, Cosmetics Microbiology, Chemical Microbiology solution, Food Microbiology Solution, and Pharmaceutical Microbiology Solution. Contact US at https://www.tmmedia.in/content/xld-agariso-65792002 Call @ +91-11-71239900 Email us @ customercare@tmmedia.in

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Manufacturer Address: A- 902A RIICO Industrial Area Phase III Bhiwadi-301019. Corp office Address: 903-9099 th Floor Bigjos Tower Netaji Subhash Place Delhi-110034. Page 1 www.titanmedia.in PRODUCT DATA SHEET INTENDED USE XLD Agar For selective isolation and enumeration of Salmonella typhi and other Salmonella species. COMPOSITION Ingredients Gm\Ltr. Agar 15.000 Sucrose 7.500 Lactose 7.500 Sodium thiosulphate 6.800 L-Lysine 5.000 Sodium chloride 5.000 Xylose 3.500 Yeast extract 3.000 Sodium deoxycholate 2.500 Ferric ammonium citrate 0.800 Phenol red 0.080 PRODUCT SUMMARY AND EXPLANATION XLD Agar was formulated by Taylor for the isolation and differentiation of enteric pathogens including Salmonella typhi from other Salmonella species. XLD Agar has been recommended for the identification of Enterobacteriaceae and for the microbiological testing of foods water and dairy products. The media formulation does not allow the over growth of other organisms over Salmonella and Shigella. XLD Agar is one of the media used in the Microbial Limit Tests in the USP EP PRINCIPLE The medium contains Yeast extracts as source of vitamins and minerals. Addition of Sodium deoxycholate acts as a selective agent which is inhibitory to Gram-positive bacteria. It suppresses the growth of other enteric pathogens and enhances the growth of only few enteric bacilli. The medium contains Xylose as a fermentable carbohydrate which is utilized by Salmonella species. The medium pH is changed due to fermentation of Xylose which is detected by indicator Phenol red thus the colony colour turns red. The medium also contains Lactose and Sucrose as the source of fermentable sugar. L- lysine is an essential amino acid source. Lysine is added to differentiate Salmonella Sp. XLD AGAR TM 492

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Manufacturer Address: A- 902A RIICO Industrial Area Phase III Bhiwadi-301019. Corp office Address: 903-9099 th Floor Bigjos Tower Netaji Subhash Place Delhi-110034. Page 2 www.titanmedia.in PRODUCT DATA SHEET Sodium chloride helps maintaining the osmotic balance of the cells. This medium also allows differentiation of bacilli based on their ability to produce H 2S it contains Sodium thiosulphate and Ferric ammonium citrate that helps visualizing the black centered colonies on production of hydrogen sulphide in the medium. Agar is added as the solidifying agent. INSTRUCTION FOR USE 1. Dissolve 56.68g in 1000ml distilled water. 2. Gently heat to boil with gentle swirling and dissolve the medium completely. DO NOT AUTOCLAVE. DO NOT RE-HEAT. 3. Cool to 45 - 50˚C prior to dispense into sterile petri plates QUALITY CONTROL SPECIFICATIONS Appearance of Powder: Light yellow to pink colour homogeneous mixture free flowing powder Appearance of prepared medium: Red colour clear to slightly opalescent gel pH at 25°C: 7.4 ± 0.2 INTERPRETATION Cultural response was observed after inoculating 10 3 CFU/ml after incubation at 35-37°C for 18-24 hours. Microorganisms ATCC Inoculum CFU/ml Growth Recovery Appearance of colony Proteus vulgaris 13315 10 3 Good ≥ 50 Grey with black centres Salmonella typhimurium 14028 10 3 Good ≥ 50 Red with black centres Salmonella paratyphi B 8759 10 3 Good ≥ 50 Red with black centres Salmonella enteritidis 13076 10 3 Good ≥ 50 Red with black centres Salmonella typhi 6539 10 3 Good ≥ 50 Red with black centres Shigella dysenteriae 13313 10 3 Good ≥ 50 Red Salmonella paratyphi A 9150 10 3 Good ≥ 50 Red Shigella sonnei 25931 10 3 Fair to Good 30 -40 Red Staphylococcus aureus 6538 10 3 Inhibited 0 - Enterococcus faecalis 29212 10 3 Inhibited 0 -

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Contact Details Manufacturer Address: A- 902A RIICO Industrial Area Phase III Bhiwadi-301019. Corp office Address: 903-9099 th Floor Bigjos Tower Netaji Subhash Place Delhi-110034. Website: https://www.tmmedia.in/content/xld-agariso-65792002 Email Id: customercaretmmedia.in Call Us: +91-11-71239900 45 Lines

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