Full Specification and Technical Data Sheet Of Potato Dextrose Agar

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"Potato Dextrose Agar infusion (BEEVER and BOLLARD 1970) promote the growth of yeasts and moulds while the low pH value partially inhibits the growth of the accompanying bacterial flora.. For more details kindly check the details given below TM Media – Titan Biotech ltd call us : +91-11-71239900, https://www.tmmedia.in/sites/default/files/TM%20344_POTATO_DEXTROSE_AGARTM344.pdf email Id: customercare@tmmedia.in, Address: 904, 9th Floor, Bigjos Tower, Naetaji Subhash Place, Netaji Subhash Place, Shakurpur, Delhi, 110034"

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PRODUCT DATA SHEET www.titanmedia.in Page 1 POTATO DEXTROSE AGAR TM 344 For cultivation and enumeration of yeasts and molds Composition Dehydrated powder hygroscopic in nature store in a dry place in tightly-sealed containers below 25°C and protect from direct Sunlight. 4.0gm of potato extract is equivalent to 200gm of infusion from potatoes Instructions for use Dissolve 39gms in 1000ml distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. Sterilize by autoclaving at 15 psi 121°C for 15 minutes. Cool at room temperature and dispense into sterile petri - plates. Appearance: Light yellow colour slightly opalescent pH at 25°C: 5.6 ± 0.2 Principle POTATO DEXTOSE AGAR is used for the isolation and enumeration and culturing of yeast and molds from samples. It can also be used in the identification of fungi and yeasts in parallel with their cellular morphology or in methods of micro cultivation in slides. Dextrose and Potato infusion BEEVER and BOLLARD 1970 promote the growth of yeasts and moulds while the low pH value partially inhibits the growth of the accompanying bacterial flora. Agar is a solidifying agent. If the medium is to be used for fungal counts and enumeration of yeasts and mold the pH should be adjusted to approximately 3.5 to inhibit bacterial count. By the addition of sterilized 10 tartaric acid solution to 1000ml of sterilized medium to obtain a pH of 3.5. Fungi grow on this medium to develop typical morphology. This general- purpose medium can be supplemented with acid or antibiotics to inhibit bacterial growth. NOTE: Do not re-heat the adjusted medium after adding the acid because the agar may hydrolyses and may not solidify. Interpretation Cultural characteristics observed after inoculating 10 3 CFU/ml on incubation at 25°C for 3 – 5 days fungi and at 30- 35°C for 72 hours yeast molds. Note: - Formerly known as Aspergillus niger. Ingredients Gms/Ltr. Dextrose 20.00 Agar 15.00 Potato infusion from 4.00 Microorganisms ATCC Inoculum CFU/ml Growth Aspergillus brasiliensis 16404 Point inoculation Good Candida albicans 10231 10 3 Good Sacchromyces cerevisiae 9763 10 3 Good Trichophyton sp. 9533 10 3 Good Trichophyton rubrum 28188 10 3 Good

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PRODUCT DATA SHEET www.titanmedia.in Page 2 References 1. Mac Faddin J. F. Media for isolation-cultivation-identification-maintenance of medical bacteria vol.1. Williams Wilkins Baltimore MD. 1985. 2. Marshall ed.. Standard methods for the examination of dairy products 16th ed. American Public Health Association Washington D.C. 1993. 3. Association of Official Analytical Chemists. Bacteriological analytical manual 8th ed. AOAC International Gaithersburg MD. 1995. 4. American Public Health Association. Recommended Methods for the Microbiological Examination of Foods. APHA. New York. 1958. 5. Bacteriological analytical manual 8th ed. AOAC International. Gaithersburg MD. European Pharmacopoeia 6th edition. 2007.

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