Bile Esculin Agar Manufacture by TM Media

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"Check Data sheet of Bile Esculin Agar and check how we can use it in our use and laboratory use. That is recommended for the isolation and identification of Y. enterocolitica, as per ISO 10273-1994. For more details kindly check the details given below TM Media – Titan Biotech ltd call us : +91-11-71239900, http://www.tmmedia.in/sites/default/files/BILE-ESCULIN-AGAR-ISO.pdf email Id: customercare@tmmedia.in, Address: 904, 9th Floor, Bigjos Tower, Naetaji Subhash Place, Netaji Subhash Place, Shakurpur, Delhi, 110034"

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PRODUCT DATA SHEET www.titanmedia.in TM 1882/1August. 2017 Page 1 BILE ESCULIN AGAR as per ISO TM 1882 For isolation and identification of Yersinia enterocolitica from food and animal feeding stuff. ISO 10273 : 1994 Composition Ingredients Gms/Ltr Bile salts 40.000 Agar 15.000 Peptic digest of animal tissue 5.000 Beef extract 3.000 Esculin 1.000 Ferric citrate 0.500 Instructions for Use Dissolve 64.5 grams in 1000 ml distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. Sterilize by autoclaving at 15 psi 121°C for 15 minutes. Appearance: Amber colour clear to slightly opalescent gel with bluish tinge pHat 25°C: 6.6 ± 0.2 Principle Yersinia enterocolitica is a Gram-negative bacillus belonging to the family Enterobacteriaceae. Yersinia enterocolitica is the causative agent of Yersiniosis a severe form of human gastroenteritis. Bile Esculin Agar is recommended for the isolation and identification of Y. enterocolitica as per ISO 10273-1994. Bile Esculin Agar containing 4 bile salts was formulated by Swan and modified by Facklam and Moody. Bile Esculin Agar is also recommended by APHA for identification of Group D Streptococci. Peptic digest of animal tissue and beef extract serve as source of carbon nitrogen and essential growth factors. Enterococci and certain streptococci hydrolyze the glycoside esculin to esculetin and dextrose. Esculin reacts with an iron salt to form a dark brown or black complex. Ferric citrate is incorporated into the medium as an indicator of esculin hydrolysis and resulting esculetin formation. Bile salts are used to inhibit gram-positive bacteria other than Enterococci.

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PRODUCT DATA SHEET www.titanmedia.in TM 1882/1August. 2017 Page 2 Interpretation Cultural characteristics observed after incubation at 35-37°C for 18-24 hours. Organism ATCC Inoculum CFU Recovery Recovery Esculin Hydrolysis Enterococcus faecalis 29212 50 - 100 Good-Luxuriant 50 Positive reaction blackening of medium around the colony Escherichia coli 25922 50 - 100 Good 40-50 Negative reaction Enterococcus faecium 27273 50 -100 Good-Luxuriant 50 Positive reaction blackening of medium around the colony Yersinia enterocolitica 27729 50 - 100 Good-Luxuriant 50 Positive reaction blackening of medium around the colony Reference 1. International Organization for Standardization ISO 1994 Draft ISO /DIS 10273. 2. Swan A. 1954 J. Clin. Pathol. 7:160. 3. Facklam R.R. and Moody M.D. 1970 Appl. Microbiol. 202:245. 4. MacFaddin J.F. 1980 Biochemical Tests for Identification of Medical Bacteria 2nd Ed. Williams and Wilkins Baltimore. 5. Downes F. P. and Ito K. 2001 Compendium of Methods for the Microbiological Examination of Foods. 4th Ed. APHA Washington.

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