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Special Issue 5 • 2015 J Chromatograph Separat Techniq ISSN:2157-7064 JCGST an open access journal Research Article Open Access Trivedi et al. J Chromatograph Separat Techniq 2015 S6 http://dx.doi.org/10.4172/2157-7064.S6-003 Special Issue Open Access Chromatography Separation T echniques Journal of Chromatography Separation Techniques ISSN: 2157-7064 Investigation of Isotopic Abundance Ratio of Biofield Treated Phenol Derivatives Using Gas Chromatography-Mass Spectrometry Trivedi MK 1 Branton A 1 Trivedi D 1 Nayak G 1 Saikia G 2 and Jana S 2 1 Trivedi Global Inc. 10624 S Eastern Avenue Suite A-969 Henderson NV 89052 USA 2 Trivedi Science Research Laboratory Pvt. Ltd. Hall-A Chinar Mega Mall Chinar Fortune City Hoshangabad Rd. Bhopal Madhya Pradesh India Corresponding author: Snehasis Jana Trivedi Science Research Laboratory Pvt. Ltd. Hall-A Chinar Mega Mall Chinar Fortune City Hoshangabad Rd. Bhopal-462 026 Madhya Pradesh India Tel: +91-755-6660006 Fax: +91-755- 6660006 E-mail: publicationtrivedisrl.com Received August 27 2015 Accepted September 14 2015 Published September 24 2015 Citation: Trivedi MK Branton A Trivedi D Nayak G Saikia G et al. 2015 Investigation of Isotopic Abundance Ratio of Biofeld Treated Phenol Derivatives Using Gas Chromatography-Mass Spectrometry. J Chromatograph Separat Techniq S6:003. doi:10.4172/2157-7064.S6-003 Copyright: © 2015 Trivedi MK et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use distribution and reproduction in any medium provided the original author and source are credited. Abstract Butylatedhydroxytoluene BHT and 4-methoxyphenol 4-MP are phenol derivatives that are generally known for their antioxidant properties and depigmenting activities. The aim of this study was to evaluate the impact of biofeld energy treatment on the isotopic abundance in BHT and 4-MP using gas chromatography-mass spectrometry GC-MS. BHT and 4-MP samples were divided into two parts: control and treated. The control group remained untreated while the treated group was subjected to Mr. Trivedi’s biofeld treatment. Control and treated samples were characterized using GC-MS. The GC-MS data revealed that the isotopic abundance ratio of 13 C/ 12 C or 2 H/ 1 H PM+1/PM and 18 O/ 16 O PM+2/PM increased signifcantly in treated BHT and 4-MP where PM- primary molecule PM+1- isotopic molecule either for 13 C or 2 H and PM+2 is the isotopic molecule for 18 O. The isotopic abundance ratio of PM+1/PM in the treated BHT and 4-MP was increased up to 181.27 and 380.73 respectively as compared to their respective control. Moreover the isotopic abundance ratio of PM+2/PM in the treated BHT and 4-MP increased up to 185.99 and 355.33 respectively. GC-MS data suggests that the biofeld treatment signifcantly increased the isotopic abundance of 2 H 13 C and 18 O in the treated BHT and 4-MP as compared to the control. Keywords: Biofeld energy treatment Butylatedhydroxytoluene Gas chromatography-mass spectrometry 4-methoxyphenol Isotopic abundance Abbreviations GC-MS: Gas Chromatography-Mass spectrometry PM: Primary Molecule PM+1: Isotopic molecule either for 13 C/ 12 C or 2 H/ 1 H PM+2: Isotopic molecule for 18 O/ 16 O BHT: Butylatedhydroxytoluene 4-MP: 4-methoxyphenol Introduction Butylatedhydroxytoluene BHT is a crystalline stable solid but it is light-sensitive and reactive to acid chlorides acid anhydrides and oxidizing agents. BHT is used as an antioxidant in many products including food pharmaceuticals rubbers paint and petroleum products 1. Its antioxidant mechanism was well studied and divided into two steps. In the frst step it forms a stable phenoxyl radical which further forms the parent and a quinone methide QM 2. QM is a reactive electrophilic species that easily forms adducts with nucleophiles or polymerize in the next step. Adduct formation between the nucleophilic groups of the active pharmaceutical ingredients with QM in pharmaceutical formulations is very much possible 3. In food preservation BHT reacts with atmospheric oxygen preferentially rather than oxidizing food materials thereby protecting them from decomposition. It is used to preserve food odor color and favor 1. 4-methoxyphenol 4-MP is a common active pharmaceutical ingredient in topical drugs used for skin depigmentation. 4-hydroxyanisole and the retinoid tretinoin have been used individually as depigmenting agents. 4-MP is ofen mixed with tretinoin a topical retinoid 4. A common formulation for this drug is an ethanolic solution of 2 4-MP and 0.01 tretinoin by mass. BHA is a commercial mixture of mono- and di-tert-butylated products of 4-MP which is an efective anti-oxidant with maximum carry-through protection used in foodstufs 5 and pharmaceuticals 6. Many polymerization inhibitors e.g. phenols work best in the presence of oxygen because they intercept peroxyl radicals and decelerate oxygen consumption while stopping chain propagation. 4-MP is an inhibitor of this type. 4-MP has tremendous ability to quench peroxyl radicals and alkyl radicals via hydrogen abstraction mechanism which leads to the formation of a phenoxyl radical 78. Te phenoxyl radical is less reactive because it is stabilized by the resonance efect. Te antimicrobial activity of phenolic compounds is highly dependent upon the chemical structure of the molecules 9. Te bactericidal actions of substituted phenols and the normal alkyl derivatives of p-chlorophenols were examined against Gram-negative and Gram-positive bacteria 1011. Te antibacterial potential of essential oils of sweet basil Ocimum basilicum L. Lamiaceae containing 1.8 4-MP and methanol extracts was evaluated for controlling the growth range of food-borne pathogenic bacteria 12. It was reported that BHT at high levels as well as at lower levels found in foods might have anti-cancer properties possibly by damaging free radicals or by stimulating the production of enzymes that detoxify carcinogens. On the other hand 4-MP is carcinogenic to the fore-stomach 1314. Te rate of chemical reaction depends on the mass of the nucleus with diferent isotopic substitutions which slightly afect the partitioning of energy within molecules. Tese deviations from perfect chemical equivalence are termed as isotope efects. Te isotopic abundance ratio is commonly reported in terms of atom percent.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Saikia G et al. 2015 Investigation of Isotopic Abundance Ratio of Biofeld Treated Phenol Derivatives Using Gas Chromatography-Mass Spectrometry. J Chromatograph Separat Techniq S6:003. doi:10.4172/2157-7064.S6-003 Page 2 of 6 Special Issue 5 • 2015 J Chromatograph Separat Techniq ISSN:2157-7064 JCGST an open access journal For example 13 C atom percent 13 C 13 C/ 12 C+ 13 C × 100 Various applications of the isotopic abundance study includes a the distribution of contaminant sources of any molecule on a native regional and global scale b the identifcation and quantifcation of alteration reactions and c the characterization of elementary reaction mechanisms that govern product formation 15. Both of the phenol derivatives taken for this study work via the formation of free radicals. Free radicals are highly unstable species. Due to their highly oxidative characteristics these free radicals may contribute to carcinogenicity or tumorigenicity however the same reactions may combat oxidative stress. Hence the stability of phenol derivatives is important to show enhanced antioxidant properties. Tis could be enhanced by Mr. Trivedi’s unique biofeld energy treatment which is already known to alter the physical and structural properties of various living and non-living substances 16. Te National Center for Complementary and Alternative Medicine NCCAM which is part of the National Institute of Health NIH has recommended the use of Complementary and Alternative Medicine CAM therapies in the healthcare sector and about 36 of Americans regularly use some form of CAM 17. CAM includes numerous energy-healing therapies in which biofeld therapy is a form of putative energy medicine that is being widely used worldwide to improve the overall health and well-being of human beings. When an electrical signal passes through any material a magnetic feld is generated in the surrounding space 18. Humans have the ability to harness energy from the environment/universe and can then transmit in to any object living or non-living around the globe. Te objects always receive the energy and respond in a useful way. Tis process is called biofeld energy treatment. Mr. Trivedi’s unique biofeld energy treatment is also called Te Trivedi Efect ® which is known to alter the physical structural and atomic properties in various metals 19-21 and ceramics 2223 in materials science. Additionally the impact of biofeld treatment has been studied in various felds like microbiology research 1624 biotechnology research 2526 and agriculture research 2728. Our group of scientists reported that the biofeld treatment substantially altered the atomic structural and physical properties in silicon carbides 29 and carbon allotropes 30. Based on the outstanding results achieved by biofeld treatments on metals and ceramics an attempt was made to evaluate the efect of biofeld treatment on the isotopic abundance ratio of 13 C/ 12 C or 2 H/ 1 H PM+1/PM and 18 O/ 16 O PM+2/PM in BHT and 4-MP . Experimental Both BHT and 4-MP were procured from SD Fine Chem. Ltd. India. Each of the samples i.e. BHT and 4-MP were distributed into two parts where one part of each sample was referred as control sample and the other part was considered as treatment group. Te treatment group was handed over to Mr. Trivedi for biofeld treatment in sealed pack and under standard laboratory conditions. Mr. Trivedi provided the treatment through his energy transmission process to the treated group without touching the sample. Te control and treated samples were characterized using Gas Chromatography-Mass Spectrometry GC-MS. GC-MS method Te GC/MS was performed in a silica capillary column. It is equipped with a quadrupole detector with pre-flter one of the fastest widest mass ranges available for any GC/MS. Te mass spectrometer was operated in an electron ionization EI positive/negative and chemical ionization mode at the electron ionization energy of 70 eV. Mass range: 20-620 Daltons amu stability: ± 0.1 m/z mass accuracy over 48 hours. Te identifcation of analytes were done by retention time and by a comparison of the mass spectra of identifed substances with references. 2 µL was injected in splitless mode. Te carry gas N 2 fow-rate was kept constant during the run at 2 ml min -1 . Te stock standard solution of BHT and 4-MP were prepared in methanol HPLC grade to a concentration of 100 µg/mL. Working standard solutions were prepared from the stock solutions. Both the control and treated samples were injected following the same experimental protocol. For GC-MS analysis the treated sample was further divided into three parts as T1 T2 T3 and T4. Te GC-MS data was obtained in the form of abundance vs . mass to charge ratio m/z which is known as mass spectrum. Te isotopic abundance ratio 13 C/ 12 C or 2 H/ 1 H PM+1/PM and 18 O/ 16 O PM+2/PM was expressed by its deviation in treated samples as compared to the control. Te percentage change in isotopic ratio PM+1/PM and PM+2/PM were calculated on a percentage scale from the following formula: RR treated control PercentchangeinisotopicabundanceratioPM 1/PM 100 R control − + × Where R Treated and R Control are the ratio of intensity at PM+2 and PM+1 to PM in mass spectra of treated and control samples respectively. Solution preparation Compounds were dissolved in methanol HPLC grade and fltered with 0.2 micron flter tip ftted with a syringe. Phenol derivatives were polar molecule and therefore a polar solvent methanol was used as the diluent. Minimum detection limit of the instrument is up to 1 picogram masses. Working standard solutions were prepared from the stock solutions concentration 100 µg/mL. Te spectra was obtained by injecting 2 µL standard solution of the phenol components in methanol. A solvent delay of 4 minutes eliminates the appearance of the methanol solvent peak in this chromatogram. Results and Discussion GC-MS spectroscopy GC-MS spectra of BHT: Te GC-MS spectra of control treated samples T1 T2 and T3 T4 are presented in Figures 1 2 and 3 respectively. MS spectra showed PM peak at m/z 220 and base peak at m/z 205 in all control and treated BHT samples T1 T2 T3 and T4. Te intensity ratio of PM+1 i.e. m/z221 and PM i.e. m/z220 peaks are presented in Table 1. Eight major peaks at m/z 220 205 177 145 105 91 57 and 41 were observed in control sample due to degradation of BHT corresponded to the following ions respectively: C 15 H 24 O + C 14 H 21 O + C 12 H 17 O + C 10 H 9 O + C 8 H 9 + C 7 H 7 + C 4 H 9 + and C 3 H 5 + . Peak at m/z205 was observed due to C 14 H 21 O + and a methyl cation from BHT. While peaks at m/z 41 and 177 were observed due to the fragmentation of tertiary butyl group of BHT to propene and C 12 H 17 O + respectively. Peaks at m/z145 and 57 were observed due to the formation of an m-xylene derivative and butane. Finally m-xylene was observed at m/z105 in BHT fragmentation. More suggestively the treated BHT samples T1-T4 were fragmented in the same way as compared to control and showed eight major peaks in the mass spectrum with diferent intensities. All peaks m/z 220 205 177 145 105 91 57 and 41 were same for both treated and control samples 31. However a signifcant alteration in isotopic abundance ratio of PM+1/PM and PM+2/PM was observed for treated samples of BHT as compared to control. Te isotopic abundance ratio in control

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Citation: Trivedi MK Branton A Trivedi D Nayak G Saikia G et al. 2015 Investigation of Isotopic Abundance Ratio of Biofeld Treated Phenol Derivatives Using Gas Chromatography-Mass Spectrometry. J Chromatograph Separat Techniq S6:003. doi:10.4172/2157-7064.S6-003 Page 3 of 6 Special Issue 5 • 2015 J Chromatograph Separat Techniq ISSN:2157-7064 JCGST an open access journal Figure 1: GC-MS spectrum of control butylatedhydroxytoluene sample. Figure 2: GC-MS spectra of treated butylatedhydroxytoluene sample T1 and T2. Figure 3: GC-MS spectra of treated butylatedhydroxytoluene sample T3 and T4. 0.58 1.83 38.39 181.27 0 50 100 150 200 T1 T2 T3 T4 Percent change Figure 4: Percent change in isotopic abundance PM+1/PM of butylatedhydroxytoluene under biofeld treatment as compared to control. and treated BHT was calculated and presented in the Figures 4 and 5 respectively. Te isotopic abundance ratio of PM+1/PM of BHT treated sample was increased exponentially from T1 to T4 T10.58 T21.83 T338.39 and T4181.27 as compared to control. In case of PM+2 isotope same trend was followed isotopic abundance ratio of PM+2/PM was slightly decreased by 0.65 for T1 sample and then increased up to 185.99 in case of T4 T1-0.65 T22.81 T338.34 and T4185.99. Te increased isotopic abundance ratio of PM+1/PM and PM+2/PM in treated BHT may increase efective mass µ and binding energy in this molecules with heavier isotopes. GC-MS spectra of 4-MP: PM peak was observed at m/z124 in both control and treated samples with diferent intensity ratio Figures -0.65 2.81 38.34 185.99 -50 0 50 100 150 200 T1 T2 T3 T4 Percent change Figure 5: Percent change in isotopic abundance PM+2/PM of butylatedhydroxytoluene under biofeld treatment as compared to control.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Saikia G et al. 2015 Investigation of Isotopic Abundance Ratio of Biofeld Treated Phenol Derivatives Using Gas Chromatography-Mass Spectrometry. J Chromatograph Separat Techniq S6:003. doi:10.4172/2157-7064.S6-003 Page 4 of 6 Special Issue 5 • 2015 J Chromatograph Separat Techniq ISSN:2157-7064 JCGST an open access journal Figure 6: GC-MS spectrum of control sample of 4-methoxyphenol. Figure 8: GC-MS spectra of treated samples of 4-methoxyphenol T3 and T4. -8.07 380.73 104.28 103.9 -100 0 100 200 300 400 500 T1 T2 T3 T4 Percent change Figure 9: Percent change in isotopic abundance PM+1/PM of 4-methoxyphenol under biofeld treatment as compared to control. Figure 7: GC-MS spectra of treated samples of 4-methoxyphenol T1 and T2. -10.16 355.33 96.57 102.27 -100 0 100 200 300 400 T1 T2 T3 T4 Percent change Figure 10: Percent change in isotopic abundance PM+2/PM of 4-methoxyphenol under biofeld treatment as compared to control.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Saikia G et al. 2015 Investigation of Isotopic Abundance Ratio of Biofeld Treated Phenol Derivatives Using Gas Chromatography-Mass Spectrometry. J Chromatograph Separat Techniq S6:003. doi:10.4172/2157-7064.S6-003 Page 5 of 6 Special Issue 5 • 2015 J Chromatograph Separat Techniq ISSN:2157-7064 JCGST an open access journal 6-8. Te intensity ratio of PM peak and PM+1 peak are given in Table 2. Total fve major peaks at m/z124 109 81 53 and 39 were observed for both control and treated samples of 4-MP due to C 7 H 8 O 2 + C 6 H 5 O 2 + C 6 H 9 + C 4 H 5 + and C 3 H 3 + ions respectively. Peak at m/z109 and 81 were seen due to the initial fragmentation of 4-MP to hydroquinone base peak and methyl radical where hydroquinone was further reduced to cyclohexene m/z81. Peaks at 53 and 39 was observed due to the formation of 13-butadiene and propa-12-diene from the ring opening reaction of 4-MP. One low intensity peak at m/z27 was observed in all treated T1-T4 4-MP samples which was due to the formation of ethene ion C 2 H 3 + . Fragmentation pattern of both control and treated 4-MP molecules were same however number of fragmented peaks were increased from control to treated samples 32. Isotopic abundance ratio of PM+1/PM and PM+2/PM of control and treated 4-MP was calculated and presented in the Figures 9 and 10 respectively. Te isotopic abundance ratio of PM+1/PM of treated 4-MP was increased signifcantly up to 380.73 T1-8.07 T2380.75 T3104.28 and T4103.9 under biofeld treatment. Te isotopic abundance ratio of PM+2/PM was also increased in a similar way up to 355.33 T1-10.16 T2355.33 T396.57 and T4102.27. Atoms taking part in chemical bonds with higher isotopic number might have higher binding energy with increased efective mass µ and vice versa. Tus the increased isotopic abundance ratio of 13 C/ 12 C or 2 H/ 1 H and 18 O/ 16 O in both the compounds BHT and 4-MP might increase the efective mass and binding energy afer biofeld treatment that may enhance the stability of phenol derivatives signifcantly. Te increased isotopic abundance ratio of PM+1/PM and PM+2/PM in samples of BHT and 4-MP afer biofeld treatment may result in chemical stability. It could be due to nuclear level transformation of PM+1 and PM which may induced through biofeld treatment. Te observed fragmentation pattern and number fragmented peaks were same for control and treated samples of BHT and while number fragmented peaks in treated samples of 4-MP were increased. Te increased isotopic abundance ratio may reasonably increase the no of heavier isotopes i.e. 2 H 13 C and 18 O in the molecule afer biofeld treatment. If a lighter nucleus is replaced by a heavier one then corresponding efective mass µ of that particular bond could be changed accordingly. We have presented some probable bonds that might present such as 12 C- 12 C 1 H- 12 C 13 C- 12 C 2 H- 12 C 1 H- 13 C 2 H- 13 C 13 C- 13 C 12 C- 18 O 1 H- 18 O 13 C- 16 O and 2 H- 16 O in the biofeld treated molecules. Efective mass is calculated and presented in Table 3. Te result showed that µ of normal 12 C- 12 C and 1 H- 12 C bond was 6 and 0.923 respectively. It showed that reduced mass is increased in case of heavier isotope i.e. 12 C- 13 C6.26 and 2 H- 12 C1.71. Te increased µ was observed in case of 18 O- 12 C and 1 H- 18 O bonds also. From the Figures 4 5 9 and 10 it was observed that the isotopic abundance ratio of PM+1/PM in treated samples increased by 181.27 and 380.73 in BHT and 4-MP. So maximum number of isotope replacement may occur in this process which may lead to signifcant change in energy of the isotope substituted bonds. It may enhance the bond strength stability and binding energy of BHT and 4-MP molecules. Conclusions In summary BHT and 4-MP were studied under the infuence of biofeld energy treatment and observed signifcant changes in isotopic abundance compared to the control sample. Te biofeld treatment ofers a remarkable means to alter the isotopic abundance ratio of 13 C/ 12 C or 2 H/ 1 H PM+1/PM and 18 O/ 16 O PM+2/PM in BHT as well as 4-MP which have a signifcant impact on bond energies and the chemical reactivity of the molecules. Te percent change in the isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated BHT was increased up to 181.27 and 185.99 respectively as compared to the control. However the percent change in the isotopic abundance ratio of PM+1/PM and PM+2/PM in treated 4-MP was increased up to 380.73 and 355.33 respectively. GC-MS data suggests that biofeld treatment has signifcantly increased the isotopic abundance of 2 H 13 C and 18 O in treated BHT and 4-MP. Stability may be increased by increasing the efective mass µ which consequently increases the binding energy. Te increased isotopic abundance ratio afer biofeld treatment on BHT and 4-MP molecules may increase the bond stability which could result in reduce autocatalytic oxidation reactions and thus prevent ageing and most common reactions initiated by heat light and molecular oxygen. Acknowledgments The authors would like to acknowledge the whole team from the Sophisticated Analytical Instrument Facility SAIF Nagpur for providing the instrumental facility. We are very grateful for the support from Trivedi Science Trivedi Master Wellness and Trivedi Testimonials in this research work. References 1. Babich H 1982 Butylated hydroxytoluene BHT: a review. Environ Res 29: 1-29. 2. Stebbins R Sicilio F 1970 The kinetics of disproportionation of the 26-di-t- butyl-4-methyl phenoxy radical. Tetrahedron 26: 291-297. 3. Zhang F Nunes M 2004 Structure and generation mechanism of a novel degradation product formed by oxidatively induced coupling of miconazole nitrate with butylated hydroxytoluene in a topical ointment studied by HPLC- ESI-MS and organic synthesis. J Pharm Sci 93: 300-309. 4. Fleischer AB Jr Schwartzel EH Colby SI Altman DJ 2000 The combination of 2 4-hydroxyanisole Mequinol and 0.01 tretinoin is effective in improving the appearance of solar lentigines and related hyperpigmented lesions in two double-blind multicenter clinical studies. J Am Acad Dermatol 42: 459-467. 5. Mulky MJ 1976 Toxicology of oil seeds. J Oil Technol Assoc India 8: 106-111. Parameters Control Treated T1 T2 T3 T4 Peak Intensity at m/zPM 56.32 57.46 66.62 77.57 84.36 Peak Intensity at m/zPM+1 8.41 8.63 10.13 16.03 35.75 Peak Intensity at m/zPM+2 0.74 0.75 0.90 1.41 3.17 Table 1: GC-MS isotopic abundance analysis result of butylatedhydroxytoluene. Parameters Control Treated T1 T2 T3 T4 Peak Intensity at m/zPM 94.46 92.36 90.27 100 99.71 Peak Intensity at m/zPM+1 7.81 7.02 35.88 16.89 16.81 Peak Intensity at m/zPM+2 0.74 0.65 3.22 1.54 1.58 Table 2: GC-MS isotopic abundance analysis result of 4-methoxyphenol. Isotopes Bonds Isotope type Reduced mass m A m B /m A +m B 12 C- 12 C Lighter 6.00 13 C- 12 C Heavier 6.26 1 H- 12 C Lighter 0.923 1 H- 13 C Heavier 0.929 2 H- 12 C Heavier 1.71 1 H- 18 O Heavier 0.94 2 H- 16 O Heavier 1.77 18 O- 12 C Heavier 7.20 16 O- 13 C Heavier 7.17 Table 3: Possible isotopic bonds in butylatedhydroxytoluene and 4-methoxyphenol. m A : Mass of an atom A m B : Mass of an atom B A may be C or H and so on.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Saikia G et al. 2015 Investigation of Isotopic Abundance Ratio of Biofeld Treated Phenol Derivatives Using Gas Chromatography-Mass Spectrometry. J Chromatograph Separat Techniq S6:003. doi:10.4172/2157-7064.S6-003 Page 6 of 6 Special Issue 5 • 2015 J Chromatograph Separat Techniq ISSN:2157-7064 JCGST an open access journal 6. Nobuteshi K 1989 Jpn Kokai Tokkyo Koho JP 01221320 CA: 113 1990 12135n. 7. Levy LB 1985 Inhibition of acrylic acid polymerization by phenothiazine and p-methoxyphenol. J Polym Sci Part A: Polym Chem 23: 1505-1515. 8. Levy LB 1996 The inhibition of butyl acrylate by p-methoxyphenol. J Appl Polym Sci 60: 2481-2487. 9. Suter CM 1941 Relationships between the structure and bactericidal properties of phenols. Chem Rev 28: 269-299. 10. Tanner FW Wilson FL 1943 Germicidal action of aliphatic alcohols. Exp Biol Med 52: 138-140. 11. Klarmann EG Shternov VA Gates LW 1932 Halogen derivatives of monohydroxyldiphenylmethane and their antibacterial action. J Am Chem Soc 54: 3315-3328. 12. Hossain MA Kabir MJ Salehuddin SM Rahman SM Das AK et al. 2010 Antibacterial properties of essential oils and methanol extracts of sweet basil Ocimum basilicum occurring in Bangladesh. Pharm Biol 48: 504-511. 13. Tamano S Hirose M Tanaka H Asakawa E Ogawa K et al. 1992 Forestomach neoplasm induction in F344/DuCrj rats and B6C3F1 mice exposed to sesamol. Jpn J Cancer Res 83: 1279-1285. 14. Asakawa E Hirose M Hagiwara A Takahashi S Ito N 1994 Carcinogenicity of 4-methoxyphenol and 4-methylcatechol in F344 rats. Int J Cancer 56: 146- 152. 15. Weisel CP Park S Pyo H Mohan K Witz G 2003 Use of stable isotopically labeled benzene to evaluate environmental exposures. J Expo Anal Environ Epidemiol 13: 393-402. 16. Trivedi MK Patil S Shettigar H Bairwa K Jana S 2015 Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofeld treatment. J Microb Biochem Technol 7: 202-205. 17. Barnes PM Powell-Griner E McFann K Nahin RL 2004 Complementary and alternative medicine use among adults: United States 2002. Adv Data : 1-19. 18. Maxwell JC 1865 A dynamical theory of the electromagnetic feld. Phil Trans R Soc Lond 155: 459-512. 19. Trivedi MK Patil S Tallapragada RM 2012 Thought intervention through bio feld changing metal powder characteristics experiments on powder characteristics at a PM plant. Future Control and Automation LNEE 173: 247-252. 20. Trivedi MK Patil S Tallapragada RM 2015 Effect of biofeld treatment on the physical and thermal characteristics of aluminium powders. Ind Eng Manage 4: 151. 21. Trivedi MK Patil S Tallapragada RM 2013 Effect of biofeld treatment on the physical and thermal characteristics of silicon tin and lead powders. J Material Sci Eng 2: 125. 22. Trivedi MK Patil S Tallapragada RM 2013 Effect of biofeld treatment on the physical and thermal characteristics of vanadium pentoxide powder. J Material Sci Eng S11: 001. 23. Trivedi MK Nayak G Patil S Tallapragada RM Latiyal O 2015 Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio feld treatment. Ind Eng Manage 4: 161. 24. Trivedi MK Patil S Shettigar H Gangwar M Jana S 2015 Antimicrobial sensitivity pattern of Pseudomonas fuorescens after biofeld treatment. J Infect Dis Ther 3: 222. 25. Patil SA Nayak GB Barve SS Tembe RP Khan RR 2012 Impact of biofeld treatment on growth and anatomical characteristics of Pogostemon cablin Benth. Biotechnology 11: 154-162. 26. Nayak G Altekar N 2015 Effect of biofeld treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9. 27. Shinde V Sances F Patil S Spence A 2012 Impact of biofeld treatment on growth and yield of lettuce and tomato. Aust J Basic Appl Sci 6: 100-105. 28. Lenssen AW 2013 Biofeld and fungicide seed treatment infuences on soybean productivity seed quality and weed community. Agricultural Journal 8: 138-143. 29. Sances F Flora E Patil S Spence A Shinde V 2013 Impact of biofeld treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35. 30. Cunha CD Leite SGF 2000 Gasoline biodegradation in different soil microscoms. Braz J Microbiol 31: 45-49. 31. Webbook 2015 Butylated Hydroxytoluene. http://webbook.nist.gov/cgi/cbook. cgiIDC128370Mask200Mass-Spec. Accessed on: September 2015. 32. Webbook 2015 Mequinol. http://webbook.nist.gov/cgi/cbook. cgiIDC150765Mask200Mass-Spec. Accessed on: September 2015. Citation: Trivedi MK Branton A Trivedi D Nayak G Saikia G et al. 2015 Investigation of Isotopic Abundance Ratio of Biofeld Treated Phenol Derivatives Using Gas Chromatography-Mass Spectrometry. J Chromatograph Separat Techniq S6:003. doi:10.4172/2157-7064.S6-003 OMICS International: Publication Benefits Features Unique features: • Increased global visibility of ar ticles thr ough w or ld wide distribution and inde xing • Sho w casing recent researc h output in a timely and updated manner • Special issues on the current trends of scientifc researc h Special features: • 700 Open Access Journals • 50000 editorial team • R apid revie w pr ocess • Quality and quic k editorial revie w and publication pr ocessing • Inde xing at PubMed par tial Scopus EBSCO Inde x Coper nicus Google Sc holar etc. • Sharing Option: Social Netw or king Enabled • Authors R evie w ers and Editors re w arded with online Scientifc Credits • Better discount f or y our subsequent ar ticles Submit y our manuscript at: http://www .omicsonline .org/submission

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