Multidrug Resistant of Raoultella Ornithinolytica

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The present study aimed to evaluate the impact of Mr. Trivedi’s biofield treatment onantimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical study, and biotype number of multidrug resistant strain of R. ornithinolytica.

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Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica Mahendra Kumar Trivedi 1 Shrikant Patil 1 Harish Shettigar 1 Mayank Gangwar 2 and Snehasis Jana 2 1 Trivedi Global Inc. 10624 S Eastern Avenue Suite A-969 Henderson NV 89052 USA 2 Trivedi Science Research Laboratory Pvt. Ltd Madhya Pradesh India Corresponding author: Jana S Trivedi Science Research Laboratory Pvt. Ltd Fortune City Hoshangabad Rd. Bhopal Madhya Pradesh India Tel: +91-755-6660006 E-mail: publicationtrivedisrl.com Received date: June 6 2015 Accepted date: July 23 2015 Published date: July 30 2015 Copyright: © 2015 Trivedi MK et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use distribution and reproduction in any medium provided the original author and source are credited. Abstract Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. As biofield therapy is increasingly popular in biomedical heath care so present study aimed to evaluate the impact of Mr. Trivedi’s biofield treatment on antimicrobial sensitivity minimum inhibitory concentration MIC biochemical study and biotype number of multidrug resistant strain of R. ornithinolytica. Clinical sample of R. ornithinolytica was divided into two groups i.e. control and biofield treated which were analyzed for the above parameters using MicroScan Walk-Away® system on day 10 after treatment. Antimicrobial sensitivity assay results showed a significant increase 60.71 in sensitivity pattern of antimicrobials i.e. changed from resistant to susceptible while 10.71 of tested antimicrobials changed from intermediate to susceptible as compared to control. MIC results showed a significant decrease in MIC values of 71.88 tested antimicrobials as compared to control. Biochemical reaction study showed 15.15 alteration in different biochemical such as cetrimide cephalothin kanamycin and ornithine after biofield treatment as compared to control. A significant change in biotype number 7775 4370 was also observed with organism identified as Klebsiella oxytoca after biofield treatment as compared to control 7775 5372. Overall results conclude that biofield treatment could be used as complementary and alternative treatment strategy against multidrug resistant strain of R. ornithinolytica with respect to improve the sensitivity and reduce the MIC values of antimicrobials. Hence it is assumed that biofield treatment might be a suitable cost effective treatment strategy in near future which could have therapeutic value in patients suffering from multidrug resistant pathogens. Keywords: Raoultella ornithinolytica Biofield treatment Antimicrobial susceptibility Biochemical reaction Biotype Multidrug resistant Introduction Genus Raoultella belongs to the family Enterobacteriaceae mainly contains Gram-negative aerobic non-motile capsulated and facultative anaerobic bacilli 1. Initially genus Raoultella was classified in Klebsiella further on the basis of 16S rRNA and rpoB genes study includes it in new genus Raoultella. It includes Raoultella ornithinolytica R. electrica R. planticola and R. terrigena. R. ornithinolytica is widely distributed in aquatic flora fishes and insects but it was associated with histamine poisoning in fish 2. Human infections are often less reported earlier but different cases of bacteremia are reported due to R. terrigena 3 R. planticola 4 and R. ornithinolytica 56. Although most of the infectious cases are often associated with underlying existing infection especially malignancies. However other pathogenic cases such as urinary tract infection soft issue infections in adults and neonatal infections are also reported 7. Thus this is very clear from the above reports that Raoultella genus acts as human pathogen and multidrug resistant MDR strain will cause a serious threat to human health causing pneumonia and other infections 8. Broad spectrum antimicrobials and combinations therapies are the only treatment strategy to cure the infection of MDR. Antimicrobials are always associated with serious side effects 9. Currently no such alternative therapies apart from medicines are available against MDR microorganism infections biofield treatment may be a new approach to improve the susceptibility pattern of R. ornithinolytica. The National Center for Complementary and Alternative Medicine NCCAM places biofield therapy under subcategory of energy therapies as one of the five complementary medicine domain. It is scientifically preferred term for the biologically produced electromagnetic and subtle energy field that provides regulatory and communication functions within the organism. The cumulative effect of bio-magnetic and electric field that surrounds the human body is defined as biofield energy. However the energy can exists in several forms such as kinetic potential electrical magnetic and nuclear. But human body has the power to produce measurable electric and magnetic signals 1011. Similarly the human nervous system consists of the energy and chemical information in the form of electrical signals. Thus human has the ability to harness the energy from environment or universe and can transmit into any living or nonliving objects around the globe. The objects always receive the energy and responding into useful way via biofield energy and the process is known as biofield treatment. Even every cell of human body will produce minute amount of magnetic and electric field as it always covers with positive Alternative Integrative Medicine Trivedi et al. Altern Integr Med 2015 4:3 http://dx.doi.org/10.4172/2327-5162.1000193 Research Article Open Access Altern Integr Med ISSN:2327-5162 AIM an open access journal Volume 4 • Issue 3 • 1000193

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and negative charge in outer and inner cell wall respectively 12. The biofield energy can be monitored by using electromyography EMG electrocardiography ECG and electroencephalogram EEG 13. Mr. Trivedi’s biofield treatment is well known and significantly studied in different fields such as altering the sensitivity pattern of different human pathogens 14-16. It has been significantly studied in field of agriculture 17-19 biotechnology 2021 and in material science 22-24. Materials and Methods Experimental design and biofield treatment MDR strain of R. ornithinolytica was obtained from stored stock cultures of clinical sample in Microbiology Lab Hinduja Hospital Mumbai. MDR strain was divided in two groups i.e. control and treatment. In case of treatment group sealed pack of MDR strain of R. ornithinolytica was handed over to Mr. Trivedi for biofield treatment under laboratory conditions. Mr. Trivedi provided the treatment through his energy transmission process to the treated group without touching the sample. The biofield treated sample was returned in the similar sealed condition and further analyzed on day 10 using the standard protocols. After biofield treatment following parameters like antimicrobial susceptibility MIC values biochemical reactions and biotype number were measured using MicroScan Walk-Away® system Dade Behring Inc. USA with respect to control. All antimicrobials and biochemicals were procured from Sigma Aldrich. Evaluation of antimicrobial susceptibility assay Antimicrobial susceptibility pattern of R. ornithinolytica was studied using MicroScan Walk-Away® system along with Negative Break Point Combo NBPC 30 panel as per manufacturers instructions. The antimicrobial susceptibility pattern S: Susceptible I: Intermediate and R: Resistant and minimum inhibitory concentration MIC values were determined by observing the lowest antimicrobial concentration showing growth inhibition. The antimicrobials used in the susceptibility assay viz. amikacin amoxicillin/k-clavulanate ampicillin/sulbactam ampicillin aztreonam cafazolin cefepime cefotaxime cefotetan cefoxitin ceftazidime ceftriaxone cefuroxime cephalothin chloramphenicol ciprofloxacin ESBL-a Scrn ESBL-b Scrn gatifloxacin gentamicin imipenem levofloxacin meropenem moxifloxacin nitrofurantoin norfloxacin piperacillin piperacillin/tazobactam tetracycline ticarcillin/k-clavulanate and tobramycin trimethoprim/ sulfamethoxazole 25. Biochemical study Biochemical study of R. ornithinolytica was determined using MicroScan Walk-Away® system in both control and treated groups. Biochemicals used in the study are acetamide adonitol arabinose arginine cetrimide cephalothin citrate colistin esculin hydrolysis nitrofurantoin glucose hydrogen sulfide indole inositol kanamycin lysine malonate melibiose nitrate oxidation-fermentation galactosidase ornithine oxidase penicillin raffinose rhaminose sorbitol sucrose tartarate tryptophan deaminase tobramycin urea and Voges-Proskauer 25. Identification by biotype number The biotype number of R. ornithinolytica in control and treated samples were determined followed by identification of microorganism by MicroScan Walk-Away® processed panel report with the help of biochemical reaction data 25. Results Antimicrobial susceptibility Results of antimicrobial sensitivity pattern and MIC values of control and biofield treated MDR strain of R. ornithinolytica are summarized in Tables 1 and 2 respectively. All these changes were observed on day 10 after the biofield treatment as compared to control group. Antimicrobial sensitivity assay showed that 60.71 of tested 28 antimicrobials were reported with significant increase in sensitivity pattern from R → S viz. amoxicillin/k-clavulanate ampicillin/ sulbactam aztreonam cefazolin cefotaxime ceftriaxone cefuroxime cephalothin ciprofloxacin gatifloxacin gentamicin levofloxacin moxifloxacin piperacillin tetracycline trimethoprim/ sulfamethoxazole and tobramycin after biofield treatment. Apart from this 10.71 tested antimicrobials showed altered sensitivity pattern from I → S in ceftazidime piperacillin/tazobactam and ticarcillin/k- clavulanate as compared to control. Rest of the antimicrobials 28.7 did not show any alteration in sensitivity pattern Table 1. S. No. Antimicrobial Control Treated 1 Amikacin S S 2 Amoxicillin/k-clavulanate R S 3 Ampicillin/sulbactam R S 4 Ampicillin R R 5 Aztreonam R S 6 Cefazolin R S 7 Cefepime S S 8 Cefotaxime R S 9 Cefotetan S S 10 Cefoxitin S S 11 Ceftazidime I S 12 Ceftriaxone R S 13 Cefuroxime R S 14 Cephalothin R S 15 Chloramphenicol S S 16 Ciprofloxacin R S 17 Gatifloxacin R S 18 Gentamicin R S 19 Imipenem S S 20 Levofloxacin R S Citation: Trivedi MK Patil S Harish S Gangwar M Jana S 2015 Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica. Altern Integr Med 4: 193. doi:10.4172/2327-5162.1000193 Page 2 of 6 Altern Integr Med ISSN:2327-5162 AIM an open access journal Volume 4 • Issue 3 • 1000193

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21 Meropenem S S 22 Moxifloxacin R S 23 Piperacillin R S 24 Piperacillin/tazobactam I S 25 Tetracycline R S 26. Ticarcillin/k-clavulanate I S 27. Tobramycin R S 28. Trimethoprim/sulfamethoxazole R S R: Resistant I: Intermediate S: Susceptible Table 1: Effect of biofield treatment on Raoultella ornithinolytica to antimicrobial susceptibility. Besides antimicrobial sensitivity assay an overall significant decrease in MIC values were reported in 71.88 of antimicrobials twenty three out of thirty two against R. ornithinolytica as compared to control. Four folds decreased in MIC value were observed in case of cefotaxime ceftriaxone piperacillin piperacillin/tazobactam ticarcillin/k-clavulanate and cefuroxime while two folds decreased in MIC value in case of amoxicillin/clavulanic acid ampicillin/sulbactam aztreonam cefazolin cephalothin ceftazidime gentamicin norfloxacin tetracycline tobramycin gatifloxacin levofloxacin moxifloxacin and ciprofloxacin. Trimethoprim/sulfamethoxazole was reported with decreased MIC value less than 2/38 µg/mL as compared to control Figure 1 and Table 2. Figure 1: Percentage change in antimicrobial sensitivity pattern minimum inhibitory concentration MIC values and biochemical reactions after biofield treatment of multidrug resistant strain of Raoultella ornithinolytica. S. No. Antimicrobial Control µg/mL Treated µg/mL 1 Amikacin ≤16 ≤16 2 Amoxicillin/k-clavulanate 16/8 ≤8/4 3 Ampicillin/sulbactam 16/8 ≤8/4 4 Ampicillin 16 16 5 Aztreonam 16 ≤8 6 Cefazolin 16 ≤8 7 Cefepime ≤8 ≤8 8 Cefotaxime 32 ≤8 9 Cefotetan ≤16 ≤16 10 Cefoxitin ≤8 ≤8 11 Ceftazidime 16 ≤8 12 Ceftriaxone 32 ≤8 13 Cefuroxime 16 ≤4 14 Cephalothin 16 ≤8 15 Chloramphenicol ≤8 ≤8 16 Ciprofloxacin 2 ≤1 17 ESBL-a Scrn 4 ≤4 18 ESBL-b Scrn 1 ≤1 19 Gatifloxacin 4 ≤2 20 Gentamicin 8 ≤4 21 Imipenem ≤4 ≤4 22 Levofloxacin 4 ≤2 23 Meropenem ≤4 ≤4 24 Moxifloxacin 4 ≤2 25 Nitrofurantoin ≤32 ≤32 26 Norfloxacin 8 ≤4 27 Piperacillin 64 ≤16 28 Piperacillin/tazobactam 64 ≤16 29 Tetracycline 8 ≤4 30 Ticarcillin/k-clavulanate 64 ≤16 31 Tobramycin 8 ≤4 32 Trimethoprim/sulfamethoxazole 2/38 ≤2/38 ESBL-a b Scrn: Extended-Spectrum β-Lactamase screen. Table 2: Minimum inhibitory concentration MIC of tested antimicrobials on Raoultella ornithinolytica. Biochemical reaction Table 3 summarizes the data related to biochemical reactions in control and biofield treated group. Results showed that 15.15 tested biochemical reactions out of 33 were changed from positive + to negative - such as cetrimide cephalothin kanamycin ornithine and tobramycin after biofield treatment as compared with control Figure 1. Citation: Trivedi MK Patil S Harish S Gangwar M Jana S 2015 Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica. Altern Integr Med 4: 193. doi:10.4172/2327-5162.1000193 Page 3 of 6 Altern Integr Med ISSN:2327-5162 AIM an open access journal Volume 4 • Issue 3 • 1000193

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S. No. Code Biochemical Control Treated 1 ACE Acetamide - - 2 ADO Adonitol + + 3 ARA Arabinose + + 4 ARG Arginine - - 5 CET Cetrimide + - 6 CF8 Cephalothin + - 7 CIT Citrate + + 8 CL4 Colistin - - 9 ESC Esculin hydrolysis + + 10 FD64 Nitrofurantoin - - 11 GLU Glucose + + 12 H2S Hydrogen sulfide - - 13 IND Indole + + 14 INO Inositol + + 15 K4 Kanamycin + - 16 LYS Lysine + + 17 MAL Malonate + + 18 MEL Melibiose + + 19 NIT Nitrate + + 20 OF/G Oxidation-Fermentation + + 21 ONPG Galactosidase + + 22 ORN Ornithine + - 23 OXI Oxidase - - 24 P4 Penicillin + + 25 RAF Raffinose + + 26 RHA Rhaminose + + 27 SOR Sorbitol + + 28 SUC Sucrose + + 29 TAR Tartrate - - 30 TDA Tryptophan Deaminase - - 31 TO4 Tobramycin + - 32 URE Urea + + 33 VP Voges-Proskauer + + - negative + positive Table 3: Effect of biofield treatment on Raoultella ornithinolytica to the vital process of microorganism. Organism identification by biotype number Based on the biochemical results change in biotype number was observed in biofield treated group 7775 4370 at day 10 of R. ornithinolytica with respect to control 7775 5372. After alteration in biotype number the new organism was identified as Klebsiella oxytoca Table 4. Feature Control Treated Biotype 77755372 77754370 Organism Identification Raoultella ornithinolytica Klebsiella oxytoca Table 4: Effect of biofield treatment on Raoultella ornithinolytica to distinguishing feature of the genotype. Discussion MDR emergence of R. ornithinolytica is a global health problem commonly associated with bacteremia urinary tract infection neonatal infections and exist in underlying existing infection 62627. Increasing resistance in microorganism for antimicrobials becomes a major threat to health and economic problem which ultimately leads to allowing survival of the resistant bacteria and death of the susceptible ones. Major mechanistic pathways associated with resistant in microorganism are cell membrane alterations which lead to decreased uptake of drug 28 mutation occurs that lead to over expression of drug target enzymes and the other common mechanism being the drug efflux pump 29. Now-a-days R. ornithinolytica acquired resistance against broad range of antimicrobials. Research study suggests that most of the clinical isolates of R. ornithinolytica were found resistant to all class of antimicrobials such as ampicillin amoxicillin cephalothin cephotaxime chloramphenicol penicillin gentamicin rifampin and streptomycin. Nitrofurantoin and meropenam showed highest sensitivity for R. ornithinolytica in all the clinical samples 30. Our experimental control sample R. ornithinolytica showed similar sensitivity and resistant pattern of tested antimicrobials. Overall results showed increase in antimicrobial sensitivity after biofield treatment in 71.4 tested antimicrobials as compared to control. Mr. Trivedi’s biofield treatment has significantly decreased the MIC values of 71.87 tested antimicrobials as compared to control. As an enteric pathogen R. ornithinolytica causes enteric fever associated with syndrome like fever headache and abdominal pain. Best treatment therapy during the infection starts with amoxicillin/clavulanic acid for 10-14 days but therapy depends upon the sensitivity pattern of clinical strain 31. Different class of antimicrobials showed significant effect after biofield treatment viz. β- Lactam penicillins amoxicillin/clavulanic acid ampicillin/sulbactam and piperacillin cephalosporins cefazolin ceftriaxone and cefuroxime monobactum azetronan fluoroquinolones ciprofloxacin and levofloxacin aminoglycosides amikacin and tobramycin and tetracycline. Lucchetti et al. studied the effect of energy therapy as an alternate medicine on growth of bacterial culture and found a significant effect 32. Similarly experimental results showed improved antimicrobial sensitivity and reduced MIC values suggest that biofield treatment could be a new alternative treatment approach to inhibit the growth of pathogenic bacteria. Several phenotypic biochemical identification tests were available to differentiate the Raoultella species. Identification test of R. ornithinolytica was performed using a series of biochemical reactions Citation: Trivedi MK Patil S Harish S Gangwar M Jana S 2015 Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica. Altern Integr Med 4: 193. doi:10.4172/2327-5162.1000193 Page 4 of 6 Altern Integr Med ISSN:2327-5162 AIM an open access journal Volume 4 • Issue 3 • 1000193

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and the basic positive reactions were observed in case of urea ornithine and lysine decarboxylase citrate glucose and sucrose. It generally grown at 10°C with utilization of L-sorbose as a carbon source 33. Experimental control group showed positive reaction in above general characteristic of R. ornithinolytica but biofield treatment group showed a significant alteration i.e. negative reactions in biochemical such as cetrimide cephalothin kanamycin ornithine and tobramycin. Further these biochemical changes were studied by biotype number analysis which was performed using an automated system. A significant changed in biotype number was found in treated group on day 10 and new organism was identified as Klebsiella oxytoca after biofield treatment as compared to control R. ornithinolytica Table 4. Biofield treatment on pathogenic microorganism had been reported which alter the biochemical reactions followed by change biotype number and identification of new microorganism after treatment. Current results are well supported with recent reported study 34. Overall results of antimicrobial assay suggest that Mr. Trivedi’s biofield treatment has significantly improved the sensitivity and MIC value of most of the tested antimicrobials Figure 1. Therefore it may be possible that lower dose of antimicrobials might require with similar response after biofield treatment which may minimize the side effects associated with higher doses of antimicrobials. Mr. Trivedi has the ability to harness energy from environment and altered the significant changes in microorganisms 1516. Biofield treatment might be responsible to do alteration in microorganism at genetic level and/or enzymatic level which may act on receptor protein. While altering receptor protein ligand-receptor/protein interactions may alter that could lead to show different phenotypic characteristics. Biofield treatment might induce significant changes in MDR strain of R. ornithinolytica so that tested antimicrobials were showed better susceptibility pattern decreased MIC values and altered biochemical reactions against this microorganism. Conclusion Present study concludes that biofield treatment has the ability to inhibit the microbial growth by significantly increasing the susceptibility pattern and decreasing the MIC values of 71 tested antimicrobials. Biofield treatment has significantly altered the biochemical reactions and biotype number of MDR strain of R. ornithinolytica. On the basis of changed biotype number after biofield treatment new organism was identified as Klebsiella oxytoca. It is assumed that biofield treatment could be applied in biomedical health care system in future to improve the antimicrobial potency that enhance human well-being. Acknowledgement Authors gratefully acknowledged the whole team of PD Hinduja National Hospital and MRC Mumbai Microbiology Lab for their support.The generous support of Trivedi Science™ Trivedi Master Wellness™ and Trivedi Testimonials is gratefully acknowledged. References 1. Drancourt M Bollet C Carta A Rousselier P 2001 Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultellagen. nov. with description of Raoultella ornithinolytica comb. nov. Raoultella terrigena comb. nov. and Raoultella planticola comb. nov. Int J Syst Evol Microbiol 51: 925-932. 2. Kanki M Yoda T Tsukamoto T Shibata T 2002 Klebsiella pneumoniae produces no histamine: Raoultella planticola and Raoultella ornithinolytica strains are histamine producers. Appl Environ Microbiol 68: 3462-3466. 3. 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Alternative medicine: expanding medical horizons Washington DC US Government Printing Office NIH Publication No. 094-066. 11. Thomas AH 2012 Hidden in plain sight: The simple link between relativity and quantum mechanics. Swansea UK: CreateSpace Independent Publishing Platform. 12. Dale C 2009 The subtle body: An encyclopedia of your energetic anatomy. Boulder CO: Sounds True Inc. 13. Movaffaghi Z Farsi M 2009 Biofield therapies: Biophysical basis and biological regulations. Complement Ther Clin Pract 15: 35-37. 14. Trivedi MK Bhardwaj Y Patil S Shettigar H Bulbule A 2009 Impact of an external energy on Enterococcus faecalis ATCC-51299 in relation to antibiotic susceptibility and biochemical reactions-an experimental study. J Accord Integr Med 5: 119-130. 15. Trivedi MK Patil S 2008 Impact of an external energy on Staphylococcus epidermis ATCC-13518 in relation to antibiotic susceptibility and biochemical reactions-an experimental study. J Accord Integr Med 4: 230-235. 16. Trivedi MK Patil S 2008 Impact of an external energy on Yersinia enterocolitica ATCC-23715 in relation to antibiotic susceptibility and biochemical reactions: An experimental study. Internet J Alternat Med 6. 17. Shinde V Sances F Patil S Spence A 2012 Impact of biofield treatment on growth and yield of lettuce and tomato. Aust J Basis Appl Sci 6: 100-105. 18. Sances F Flora E Patil S Spence A Shinde V 2013 Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35: 22-29. 19. Lenssen AW 2013 Biofield and fungicide seed treatment influences on soybean productivity seed quality and weed community. Agricultural Journal 8: 138-143. 20. Altekar N Nayak G 2015 Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9. 21. Patil SA Nayak GB Barve SS Tembe RP Khan RR 2012 Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin Benth.. Biotechnology 11: 154-162. 22. Trivedi MK Tallapragada RR 2008 A transcendental to changing metal powder characteristics. Met Powder Rep 63: 22-28. 23. Dabhade VV Tallapragada RR Trivedi MK 2009 Effect of external energy on atomic crystalline and powder characteristics of antimony and bismuth powders. Bull Mater Sci 32: 471-479. 24. Trivedi MK Tallapragada RR 2009 Effect of superconsciousness external energy on atomic crystalline and powder characteristics of carbon allotrope powders. Mater Res Innovations 13: 473-480. Citation: Trivedi MK Patil S Harish S Gangwar M Jana S 2015 Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica. Altern Integr Med 4: 193. doi:10.4172/2327-5162.1000193 Page 5 of 6 Altern Integr Med ISSN:2327-5162 AIM an open access journal Volume 4 • Issue 3 • 1000193

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25. Fader RC Weaver E Fossett R Toyras M Vanderlaan J et al. 2013 Multilaboratory study of the biomic automated well-reading instrument versus MicroScan WalkAway for reading MicroScan antimicrobial Susceptibility and identification panels. J Clin Microbiol 51: 1548-1554. 26. Mau N Ross LA 2010 Raoultella ornithinolytica bacteremia in an infant with visceral heterotaxy. Pediatr Infect Dis J 29: 477-478. 27. Garcia-Lozano T Pascual Pla FJ Aznar Oroval E 2013 Raoultella ornithinolytica in urinary tract infections. Clinical and microbiological study of a series of 4 oncologic patients. Med Clin Barc 141: 138-139. 28. Tenover FC 2006 Mechanisms of antimicrobial resistance in bacteria. Am J Infect Control 119: S3-S10. 29. Alekshun MN Levy SB 2007 Molecular mechanisms of antibacterial multidrug resistance. Cell 128: 1037-1050. 30. Al-Hulu SM Al-Charrakh AH Al-Saadi MAK 2009 Isolation and characterization of Raoultella ornithinolytica from clinical specimens in hilla city. Iraq Med J Babylon 7: 42-47. 31. Morais VP Daporta MT Bao AF Campello MG 2009 Enteric fever-like syndrome caused by Raoultella ornithinolytica Klebsiella ornithinolytica. J Clin Microbiol 47: 868-869. 32. Lucchetti G de Oliveira RF Goncalves JP Ueda SM Mimica LM et al. 2013 Effect of Spiritist "passe" Spiritual healing on growth of bacterial cultures. Complement Ther Med 21: 627-632. 33. Hansen DS Aucken HM Abiola T Podschun R 2004 Recommended test panel for differentiation of Klebsiella species on the basis of a trilateral interlaboratory evaluation of 18 biochemical tests. J Clin Microbiol 42: 3665-3669. 34. Trivedi MK Patil S Shettigar H Gangwar M Jana S 2015 Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222. Citation: Trivedi MK Patil S Harish S Gangwar M Jana S 2015 Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica. Altern Integr Med 4: 193. doi:10.4172/2327-5162.1000193 Page 6 of 6 Altern Integr Med ISSN:2327-5162 AIM an open access journal Volume 4 • Issue 3 • 1000193

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