Slide 1: Interaction of cells with the environment Unit Outline: What is outside the cell and how do cells communicate with what’s “out there”? How do cells interact with their external environment? How do cells interact with each other?
Slide 2: Extracellular space
Slide 3: Extracellular space basal surface of ectodermal cell of chick embryo apical surface of epithelial cell from intestinal lining
Slide 4: Extracellular matrix epidermis pulled away from basement membrane basement membrane between blood vessels of glomerus and renal tubules collagen
Slide 5: Extracellular matrix
Slide 6: Extracellular matrix Proteins of ECM - collagen
Slide 7: Extracellular matrix Proteins of ECM - proteoglycans
Slide 8: Extracellular matrix Proteins of ECM - fibronectin
Slide 9: Extracellular matrix Proteins of ECM - laminin collagen laminin
Slide 10: Interaction of cells with ECM inactive INTEGRINS
Slide 11: Interaction of cells with ECM INTEGRINS functions of outside-in signals differentiation motility growth survival ligands have RGD motif
Slide 12: Interaction of cells with ECM FOCAL ADHESIONS FA
Slide 13: Interaction of cells with ECM FOCAL ADHESIONS
Slide 14: Interaction of cells with ECM HEMIDESMOSOMES
Slide 15: Interaction of cells with ECM
Slide 16: Interaction of cells with cells CELL ADHESION MOLECULES
Slide 17: Interaction of cells with cells CELL ADHESION MOLECULES
Slide 18: Interaction of cells with cells CELL ADHESION MOLECULES
Slide 19: Interaction of cells with ECM and other cells CELL ADHESION MOLECULES VS ECM ATTACHMENT MOLECULES
Slide 20: vocabulary extracellular space extracellular matrix basement membrane glycocalyx collagen fibronectin proteoglycan GAG chains laminin inside-out signaling outside-in signaling focal adhesion focal adhesion kinase (FAK) actin keratin talin vinculin actinin hemidesmosome cell adhesion molecule selectin lectin Immunoglobulin superfamily cadherin tight junction adherins junction desmosome gap junction
Slide 21: Extracellular Matrix Unit Learning Goals: the extracellular space: discuss the concept that the physical interactions between cells and their extracellular environment is highly specific as a result of molecular interactions between molecules on the cell with molecules in the matrix between cells and molecules on neighboring cells describe the molecular components of the extracellular matrix (ECM) and discuss the cellular processes regulated by interactions between cells and their extracellular environment
Slide 22: interaction of cells with ECM: compare the structure, function and signaling activity of integrins and “true” ECM proteins like collagen, laminin, and proteoglycans comapre the structure and functions of molecular structures formed at the site of attachment of cells to an artificial substrate (focal adhesion) and to the basement membrane (hemidesmosome)
Slide 23: interaction of cells with other cells: describe the general class of proteins mediating interactions between cells (cell adhesion molecules) using specific examples compare the functions of cell adhesion molecules and extracellular matrix attachment molecules discuss why insoluble solutes, whch cannot freely diffuse through the plasma membrane, also cannot pass freely between cells of an epithelial layer, and what would happen to tissues of they could
Slide 24: d. compare tight junctions, adherins junctions, desmosomes , and gap junctions in terms of structure, function and the type of cells in which they are found e. draw, in cross section, two neighboring epithelial cells and illustrate the relative location and general structure of adherins junctions, desmosomes, tight junctions and gap junctions