Genetic Technology

Genetic Technology : 

Genetic Technology By: Mr. B. del Rosario 3rd Quarter BIOLOGY Paref-Southridge School

What is genetic technology? : 

What is genetic technology? Simply put, it is the intent to increase the frequency of desired alleles in a population.

Possible benefits… : 

Possible benefits… 1947 milk cows vs. 1997 milk cows

Inbreeding develops pure lines. : 

Inbreeding develops pure lines.

Hybrids are usually bigger and better. : 

Hybrids are usually bigger and better.

Why do a test cross? : 

Why do a test cross?

Genetic Engineering : 

Genetic Engineering allows increased frequency of a desired allele in a faster, more reliable manner DNA fragments are cut/cleaved, then inserted into a host (same or different species) also referred to as recombinant DNA technology

Recombinant DNA : 

Recombinant DNA made by connecting or recombining DNA fragments from different sources

Transgenic Organisms : 

Transgenic Organisms host organism uses the foreign DNA as if it were its own Tobacco plant with a gene from a firefly

Recombinant DNA Technology : 

Recombinant DNA Technology 1st step - isolate the foreign DNA fragment to be inserted. 2nd step – attach DNA to a carrier 3rd step – transfer into the host organism

Restriction Enzymes : 

Restriction Enzymes allows DNA to be cut (a.k.a. DNA-cleaving enzymes); are bacterial proteins Some cut across both strands and produce “blunt ends.” Some cut palindromic sequences that result to the formation of “sticky ends.”

Blunt Ends : 

Blunt Ends

EcoRI action : 

EcoRI action

Commonly used vectors : 

Commonly used vectors Plasmid (of bacteria) Viruses Shotgun technique (blindly shoots tiny particles coated with the gene into the host cells) Micropipette * Vectors may be biological or mechanical.

Micropipette : 

Micropipette

Gene gun : 

Gene gun

Gene gun : 

Gene gun www.agriculture.purdue.edu/.../Genegun.jpg

What is a plasmid? : 

What is a plasmid?

Insertion into a vector : 

Insertion into a vector

Gene cloning : 

Gene cloning

Animal cloning : 

Animal cloning

Cloning : 

Cloning

Polymerase Chain Reaction (PCR) : 

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR) : 

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR) : 

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR) : 

Polymerase Chain Reaction (PCR)

References : 

References images.encarta.msn.com/.../phl/T012584A.gif www.spraguephoto.com/stock/images/El_Salvador... www.beebeesee.tv/images/SkinnyCow.jpg upload.wikimedia.org/wikipedia/commons/4/4a/C... upload.wikimedia.org/.../6/63/Horse-racing-4.jpg www.monteweston.com/Biology/Bio/collflower2.jpg www.bio.davidson.edu/.../Talbert/lucifmed.jpg www.freewebs.com/mohnbon/MouseEar.jpg

References : 

References 9. www.accessexcellence.org/.../SFTS/SFTSg/11.gif 10. www.obgynacademy.com/.../images/chimericDNA.gif 11. www.biotech.ucdavis.edu/.../micropipette.png 12. www.clpmag.com/.../2007-06/2007-06_06-02.jpg 13. fig.cox.miami.edu/.../150/gene/38x15dnagun.jpg 14. science.marshall.edu/.../images/gene%20gun.jpg 15. www.artsci.wustl.edu/~anthro/blurb/fg8.t.gif 16. employees.csbsju.edu/.../ch331/dna/plasmid.gif

References : 

References 17. www.sciencecases.org/dog_cloning/cloning.gif 18. www.lifenews.com/picturecloning2.jpg 19. library.thinkquest.org/.../polystep1.gif