An Assignment on CBPP and CCPP.: An Assignment on CBPP and CCPP. SUBMITTED TO : DR. H.C. Chauhan (Astt. Professor). DEPT. OF MICROBIOLOGY]. SUBMITTED BY : Arpan panchal. ROLL NO.- 21. REGN. NO .- 02-00151-06. CONTAGIOUS BOVINE PLEUROPNEUMONIA (CBPP) : CONTAGIOUS BOVINE PLEUROPNEUMONIA (CBPP) Slide 3: Contagious bovine pleuropneumonia (CBPP) is an infectious disease of cattle caused by Mycoplasma sp. and characterized by cuprous pneumonia, pleurisy and dilation of lymph vessels. Etiology : Etiology Mycoplasma mycoides This organism was previously lnown as Asterococcus mycoies. Slide 5: SPECIES HOST DISEASE Mycopasma mycoides Sub sp. bovis Bovine CBPP Mycopasma boviginatelium Bovine Reproductive Disease Mycopasma bovirhinis Bovine Pneumonia Mycopasma bovimastitidis Bovine Mastitis Mycopasma mycoides Sub sp. capri Sheep/Goat CCPP Mycopasma agalactiae Sheep/Goat Contagious agalactiae Mycopasma hyosynoviae Pig Arthritis Mycopasma gallisepticum Poultry CRD Mycopasma synoviae Turkey Synovitis Characters of the organism: Characters of the organism The organism is pleomorphic in nature. Occurs in rings, globules, filamentous and in bizaree form( maurer , 1980). The organism can be stained by Giemsa stain. The organism can be grown in media providing 10percent serum protein. In can culturd in developed chorioallantoic membrane and tissue culture. The organism is sensitive to all environmental influences including disinfectants, heat and drying. Susceptible Hosts: Susceptible Hosts Primarily disease of cattle although rare. Natural case have been observed in buffaloes , yalk ,bison, reindeer, and antelopes. The organism can be adopted in rabbits , guineapigs , mice and hamster by subcutaneous injection. Mice is consider as a suitable host to enable isolation of Mycoplasma from natural field cases. Mode of transmission: Mode of transmission The infection spreads through inhalation of infected droplets. Inanimate materials do not help in disease transmission. Frequent coughing induces the germ to the close approximated cattle. Large number of organisms are excreted through urine. The infection may spread through inhalation of urine. Spread may also occurs through dischage from tail lesions resulting from vaccination with virulant culture. Contact transmission is also possible. Hot and humidity influences the spread of the disease. Pathogenesis : Pathogenesis The infection spreads through inhalation of droplets from infected animals. The recovered animals act as source of infection at least for a period of 3 years. After entering through respiratory tract the organism reaches to bronchioles. It may remain in retropharyngeal gland from where it may spread in whole body. The organism from bronchioles enter into interlobular septa and causes inflammation followed by edema which causes dilation and subsequent thrombosis of lymph and blood vessels prior to development of pneumonic lesions. Death occurs due to anoxia and toxaemia. Characteristic symptoms : Characteristic symptoms Fever (104-106°F) Dyspnoea Nasal discharge General malaise Drop in milk yield. Suspended rumination. Anorexia. Cough following exercise. Reluctant to move. Abducted elbow. Arched back. Characteristic symptoms : Characteristic symptoms Extended head and neck. Dilated nostrills. Panting. Expiratory grunt. Drooling of saliva. Nasal discharge. Pain on percussion. Plueritic frictional sound on auscultation. Arthritis in calf. Oedema of throat and dewlap. carrier state following recovery. Lesions : Lesions Congestion and consolidation of lungs with fibrinous adhesions in pleura Lesions: Lesions Marbled appearance on cut surfaces of lungs Lesions : Lesions Serofibrinous exudate in thoracic cavity Diagnosis: Diagnosis Diagnosis based on , History Clinical findings Culture of the organism Dark field microscopy Precipitation test Agglutination test C.F.T. Intra dermal test. FAT Animal inoculation test RID test Differential diagnosis: Differential diagnosis The disease has to be differentiated from pneumonia, pasteurellois , and parasitic pneumonia. Treatment : Treatment The drugs like sulfanamides and arsenicals can be used. Streptomycin, otc , chloramphenicol have got some effect. Tylosin tartrate have also some effect. Dose is 2-5mg per kg body weight in every 12 hour. Spiramycin and lincomycin are also very useful drugs against mycoplasma. Vaccines.: Vaccines. Pleural exudates from natural cases has been used as vaccines. Cultured organisms of reduced virulance. Avianised vaccines of low virulance. A broth culture vaccine fron 45 th passage of avianised t1 strain. Broth culture vaccine. CONTAGIOUS CAPRINE PLEURO PNEUMONIA: CONTAGIOUS CAPRINE PLEURO PNEUMONIA Slide 20: This is a contagious disease of goat having resemblance with contagious bovine pleuro pneumonia. Distribution.: Distribution. The disease is wide spread in tropical countries. And in india the disease is reported from chennai , assam, mumbai , u.p. ,bihar etc... Etiology. : Etiology. Mycoplasma mycoides sub species caparae is the causative organism of this disease. Characters of the organism: Characters of the organism The organism is pleomorphic in nature. Occurs in rings, globules, filamentous and in bizaree form( maurer , 1980). The organism can be stained by Giemsa stain. The organism can be grown in media providing 10percent serum protein. In can culturd in developed chorioallantoic membrane and tissue culture. The organism is sensitive to all environmental influences including disinfectants, heat and drying. Mode of transmission: Mode of transmission The infection is spread through inhalation. The organism do not live long out side the animal’s body. Bot fly has been found to spread this infection. Experimental transmission is possible. Clinical findings: Clinical findings Anorexia Dullness Depression Nasal discharge Abdominal respiration Fever Dry pain full cough Animal may die without any pathological changes in the lungs. Lesions: Lesions Congestion and consolidation of lungs. Thickening of the interlobuler septa. Necrosis of the pleura. Diagnosis.: Diagnosis. Diagnosis based on , History Clinical findings Culture of the organism Dark field microscopy Precipitation test Agglutination test C.F.T. Intra dermal test. FAT Animal inoculation test RID test Treatment.: Treatment. OTC, rolitetracycline, Chloramphenocol and tylosin @ 40mg/kg bwt. For 5 days. Tiamutin @ 36mg/kg bwt. Give setisfactory response. Tylosine tartrate @ 10mg/kg b.wt. i/m along with the cotrimoxazole tablet for 5 to 7 days have been suggested. Treatment with alincomycin give satisfactory result. vaccine: vaccine Live culture of M.caparae dose 0.2ml to be given at the tip of the ear. Saponin killed lyophillized vaccine can be used. Revaccination at 6month interval is recommended. Control.: Control. Slaughter all infected animals and enforcement of rigid quarantine regulation to be made. Movements of the cattle and sheep , goats should be restricted. Carrier animals should be slaughtered. Vaccination should be done in all herd. Thanks a lot…..: Thanks a lot…..