logging in or signing up Electron Transport Chain kanisius Download Post to : URL : Related Presentations : Let's Connect Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 178 Category: Entertainment License: All Rights Reserved Like it (0) Dislike it (0) Added: May 23, 2012 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Electron Transport and Oxidative Phosphorylation: Electron Transport and Oxidative Phosphorylation It all reduces down to water.Mitochondria: Mitochondria outer membrane relatively permeable inner membrane permeable only to those things with specific transporters Impermeable to NADH and FADH 2 Permeable to pyruvate Compartmentalization Kreb's and β-oxidation in matrix Glycolysis in cytosolMost energy from Redox: Most energy from Redox electrons during metabolic reactions sent to NAD and FAD Glycolysis In cytosol produces 2 NADH Pyruvate dehydrogenase reaction In mitochondrial matrix 2 NADH / glucose Krebs In mitochondrial matrix 6 NADH and 2 FADH 2 / glucoseElectron Transport Chain: Electron Transport Chain Groups of redox proteins On inner mitochondrial membrane Binding sites for NADH and FADH 2 On matrix side of membrane Electrons transferred to redox proteins NADH reoxidized to NAD + FADH 2 reoxidized to FAD4 Complexes: 4 Complexes proteins in specific order Transfers 2 electrons in specific order Proteins localized in complexes Embedded in membrane Ease of electron transfer Electrons ultimately reduce oxygen to water 2 H + + 2 e - + ½ O 2 -- H 2 OElectron Transport Chain: Electron Transport ChainComplex 1: Complex 1 Has NADH binding site NADH reductase activity NADH - NAD + NADH ---> FMN--->FeS---> ubiquinone ubiquinone ---> ubiquinone H 2 4 H + pumped/NADHElectron Transport Chain: Electron Transport ChainComplex II: Complex II succinate ---FAD—ubiquinone Contains coenzyme Q FADH 2 binding site FAD reductase activity FADH 2 -- FADElectron Transport Chain: Electron Transport ChainComplex III: Complex III ubiquinone - ubiquinone ox while cyt C gets reduced Also contains cytochromes b proton pump 4H + Adds to gradient 8 H + / NADH 4 H + / FADH 2Electron Transport Chain: Electron Transport ChainComplex IV: Complex IV reduction of oxygen cytochrome oxidase cyt a+a3 red ---> oxidized state oxygen ---> water 2 H + + 2 e - + ½ O 2 -- 2 H 2 O transfers e - one at a time to oxygen Pumps 2H + out Total of 10 H + / NADH Total of 6 H + / FADH 2Totals: Totals Proton gradient created as electrons transferred to oxygen forming water 10 H + / NADH 6 H + / FADH 2Electron Transport Chain: Electron Transport ChainGeneration of ATP: Generation of ATP Proton dependant ATP synthetase Uses proton gradient to make ATP Protons pumped through channel on enzyme From intermembrane space into matrix ~4 H + / ATP Called chemiosmotic theoryTotals: Totals NADH 10 H + X 1 ATP = 2.5 ATP 4 H + FADH 2 6 H + X 1 ATP = 1.5 ATP 4 H +Total ATP from mitochondrial matrix : Total ATP from mitochondrial matrix Pyruvate dehydrogenase NADH ……………………………….2.5 ATP Krebs 3 NADH X 2.5 ATP/NADH ……….7.5 ATP FADH 2 X 1.5 ATP / FADH 2 ……….1.5 ATP GTP X 1 ATP / GTP …………….. 1.0 ATP (from a separate reaction) Total …………….12.5 ATP (Per glucose = X 2 = 25 ATP)What about NADH from glycolysis?: What about NADH from glycolysis? NADH made in cytosol Can’t get into matrix of mitochondrion 2 mechanisms In muscle and brain Glycerol phosphate shuttle In liver and heart Malate / aspartate shuttleGlycerol Phosphate shuttle: Glycerol Phosphate shuttle http://courses.cm.utexas.edu/jrobertus/ch339k/overheads-3/ch19_glycerol-shuttle.jpgGlycerol phosphate shuttle: Glycerol phosphate shuttle In muscle and brain Each NADH converted to FADH 2 inside mitochondrion FADH 2 enters later in the electron transport chain Produces 1.5 ATPTotal ATP per glucose in muscle and brain : Total ATP per glucose in muscle and brain Gycerol phosphate shuttle 2 NADH per glucose - 2 FADH 2 2 FADH 2 X 1.5 ATP / FADH 2 ……….3.0 ATP 2 ATP in glycoysis ……………………2.0 ATP From pyruvate and Krebs 12.5 ATP X 2 per glucose …………….. 25.0 ATP Total = 30.0 ATP/ glucoseMalate – Aspartate Shuttle: Malate – Aspartate Shuttle http://courses.cm.utexas.edu/emarcotte/ch339k/fall2005/Lecture-Ch19-2/Slide14.JPGMalate – Aspartate Shuttle in cytosol: Malate – Aspartate Shuttle in cytosol In liver and heart NADH oxidized while reducing oxaloacetate to malate Malate dehydrogenase Malate crosses membraneMalate – Aspartate Shuttle in matrix: Malate – Aspartate Shuttle in matrix Malate reoxidized to oxaloacetate Malate dehydrogenase NAD + reduced to NADH NADH via electron transport yields 2.5 ATPTotal ATP per glucose in liver and heart : Total ATP per glucose in liver and heart Malate – Aspartate Shuttle 2 NADH per glucose - 2 NADH 2 NADH X 2.5 ATP / NADH…………5.0 ATP 2 ATP from glycolysis………………..2.0 ATP From pyruvate and Krebs 12.5 ATP X 2 per glucose …………….. 25.0 ATP Total = 32.0 ATP/ glucoseSummary: Summary Total ATP / glucose Muscle and brain 30.0 ATP Uses glycerol phosphate shuttle Heart and liver 32.0 ATP Uses malate aspartate shuttle You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.