Lect1

BSCI 223: General Microbiology : 

BSCI 223: General Microbiology Instructor: Dr. Sabrina Kramer srkram@umd.edu Monday-Friday Lecture: 10-10:50 am Lab:11am-1pm

Goal of today: : 

Goal of today: Talk about ELMS Go over the syllabus Review structure of class Brief introduction to microbiology and why we study it

Announcements : 

Announcements Labs start Tomorrow! Please look for signs on the lab doors indicating which lab and lab group that you’ll be in Lab Coat!!! If you are not familiar with material in Chapter 2, please review it as needed

Course Information: : 

Course Information: BSCI 105 prerequisite Fulfills Life Sciences CORE requirement Bauman, Robert. Microbiology with Diseases by Taxonomy, 2006. 2nd ed., Pearson, Benjamin Cummings. ISBN 0-8053-7679-8 Available free, via on-line  access: Lab Manual:  Exploring New Cultures,  Smith, 2006. The lab manual  is available on line in the Course ELMS area.

People involved in BSCI 223 : 

People involved in BSCI 223 You and Your lab group Threee group assignments Your TA (teaching assistant) Sandra Jacobsen Sarah Akkoush Lab logistics and preparation Laura Cathcart Me (Sabrina Kramer) srkram@umd.edu

We need to know about you! : 

We need to know about you! Name (Full name and preferred name) Preferred e-mail Year and major (if applicable) Related Bio/Chem courses taken Computer experience (Photoshop, html, wikis)? Local address: ex: on campus, Silver Spring, etc.? One word to describe you

Syllabus : 

Syllabus Class is broken into modules

Big Picture Organization : 

Big Picture Organization Module 1 Module 2 Module 3 Lectures Labs 1-10 11-19 20-27 1-7 8-13 14

Big Picture Organization : 

Big Picture Organization Module 1 Module 2 Module 3 Lectures Labs CASE Study ET microbes Transgenic corn Why is Tony sick 1-10 11-19 20-27 1-7 8-13 14

Big Picture Organization : 

Big Picture Organization Module 1 Module 2 Module 3 Lectures Labs CASE Study PAKs ET microbes Transgenic corn Why is Tony sick 1-10 11-19 20-27 1-7 8-13 14 1.1 1.2 1.3 1.4 2.1 2.2 2.3 3.1 3.2 3.3

Slide 11: 

CASE-related questions that: -I introduce in class -you answer in WebCT -we review in class -help you grasp/apply important concepts -are the basis of some exam questions What are PAKs ??? Participation Applied Knowledge Questions

Big Picture Organization : 

Big Picture Organization Module 1 Module 2 Module 3 Lectures Labs CASE Study PAKs Assignment ET microbes group-authored web-page/wiki Transgenic corn Why is Tony sick Group summary; Discussion/debate presentation to lab section 1-10 11-19 20-27 1-7 8-13 14 1.1 1.2 1.3 1.4 2.1 2.2 2.3 3.1 3.2 3.3

ELMS : 

ELMS http://elms.umd.edu Essential part of this course Online discussion, check grades, download my powerpoint lectures, online calendar of due dates, quizzes, vocabulary lists, announcements, lab manual Please try logging in this week Tech quiz in ELMS

Slide 15: 

FREE advice!!! Don’t try to copy down everything on every slide during lecture…ALL slides will be available on ELMS. Don’t get behind. -Read (ahead if possible) -Learn & use vocab. words Do: ask questions. Do: participate in class. Do: read your textbook. Do: work with your lab group partners Do: the PAK Activities - discussed in lecture (talk w/ neighbors) - some Exam questions based on these activities Do: turn off your cell phone as you enter class

What is Microbiology? : 

What is Microbiology? Study of small organisms Microbes = Microorganisms Can you name some microorganisms? Archeabacteria (Archaea) Eubacteria (bacteria) Prokaryotes Algae Fungi Protozoa Eukaryotes Viruses Obligate parasites

Why Study Microbiology? : 

Why Study Microbiology? Bacteria are dominant organisms on earth 1013 cells in our body 1014 bacteria in our body Live everywhere on our planet Make up the majority of the biomass Essential to life Fix Nitrogen Break down Carbon compounds (cellulose, chitin) Produce energy

Applications of Microbiology : 

Applications of Microbiology

Microbes are very diverse and can live in a wide range of areas. Where of the following places are microbes not normally found? : 

Microbes are very diverse and can live in a wide range of areas. Where of the following places are microbes not normally found? In pond water (~28ºC) In your freezer (~-20ºC) In a volcanic vent (~100ºC) At the bottom of the ocean (~4ºC) At the North Pole (~-10º C) In your urine (~37ºC)

Slide 20: 

Thermopilic bacteria living in Yellowstone National Park 60-80° C Psycrophilic algae in Sierra Nevada mountains -4 ° to +10 ° C

Diversity Continued : 

Diversity Continued Figure 1.6

Diversity Continued : 

Diversity Continued Fig 1.4

Diversity continued : 

Diversity continued Figure 1.9

Biosphere 2 : 

Biosphere 2 Completely environmentally closed facility Infused soil with microbes to increase photosynthesis by plants Microbes respired at a rate greater than plants produced Oxygen Result = 0.5% less oxygen per month

Which of the following statements regarding microorganisms is false? : 

Which of the following statements regarding microorganisms is false? They constitute the major portion of biomass on earth. Most of them cause disease in humans. In nature they tend to exist in assemblages called populations. They are among the oldest known life forms on earth.

Slide 26: 

Figure 4.3

Size matters! : 

Size matters! If a bacterium was the size of you, then you would be the size of about 393 empire state buildings!!!

Slide 28: 

Some gigantic prokaryotes dwarf many eukaryotic cells Figure pg 58

Serratia marcesensa bacterial species you use in lab : 

Serratia marcesensa bacterial species you use in lab Growing on Nutrient Agar in Petri dish Picture taken using electronmicroscope 6 cm 25 µm

How many bacteria are therein a colony? : 

Growing on Nutrient Agar in Petri dish 6 cm How many bacteria are therein a colony?

How many bacteria are therein a colony? : 

Growing on Nutrient Agar in Petri dish How many bacteria are therein a colony? More than a billion! 1,000,000,000 = 109

Next time: : 

Next time: Don’t forget your lab coat! Tech Quiz tomorrow evening! Introduction to Case study 1 History of Microbiology and Microscopy Readings: pp 2-18 pp 65-69 pp 94-109