logging in or signing up Staining jitpatel21 Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 105 Category: Science & Tech.. License: All Rights Reserved Like it (0) Dislike it (0) Added: July 12, 2011 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Observing Microorganisms through a Microscope: Observing Microorganisms through a Microscope Chapter 3Basic techniques needed to study Bacteria: Basic techniques needed to study Bacteria 1. Grow Bacteria 2. Isolate Bacteria 3. Grow Bacteria in pure culture 4. Observe Bacteria 5. Identify BacteriaMicroscope: Microscope Resolving Power - ability to distinguish two distinct points absolute limit of the Resolving Power is about 1/2 the wavelength of light that is used to illuminate the specimenPreparing smears for staining: Preparing smears for staining 1. Bacteria on slide 2. Air Dry 3. Bacteria are HEAT FIXED to the slide 4. Stain is appliedStaining Reaction: Staining Reaction Stains - salts composed of a positive and negative ion, one of which is colored (chromophore) Basic Dyes - chromophore is the positive ion dye+ Cl- Acid Dyes - chromophore is the negative ion Na+ dye-Bacteria are slightly negative, so are attracted to the positive chromophore of the BASIC DYE: Bacteria are slightly negative, so are attracted to the positive chromophore of the BASIC DYE Common Basic Dyes crystal violet methylene blue safranin basic fuchsinSlide 7: Acid Dyes - used for Negative Staining (background is stained) Mordant - intensifies the stain or coats a structure to make it thicker and easier to see after it is stained Example: Flagella - can not normally be seen, but a mordant can be used to increase the diameter of the flagella before it is stained Salmonella typhosaDifferential Stains: Differential Stains React differently with different types of bacteria 2 Most Common Gram Stain Acid-Fast StainGram Stain: Gram Stain 1884 Hans Christian Gram most important stain used in Bacteriology Divides all Bacteria into 2 groups: Gram (+) Gram (-)Slide 10: Gram Stain 1. Crystal violetSlide 11: Gram Stain 2. Grams Iodine (mordant)Slide 12: Gram Stain 3. AlcoholSlide 13: Gram Stain 4. Safranin (Counterstain)Results: Results Gram (+) Purple Gram (-) Red Difference - due to structure of cell wall Gram (+) Thick cell wall Gram (-) Thin cell wallIdentification of a Bacteria Unknown: Identification of a Bacteria Unknown 1. Gram Reaction 2. MorphologyAcid - Fast Stain: Acid - Fast Stain Differential Stain - divides bacteria into 2 groups Acid - Fast Non Acid - Fast Used to identify organisms in the Genera Mycobacterium (high lipid and wax content in cell wall)Slide 17: 2 Important Pathogens Mycobacterium tuberculosisSlide 18: Mycobacterium lepraeAcid - Fast Stain: Acid - Fast Stain 1. Carbolfuchsin (Red) 2. Acid Alcohol 3. Counterstain with Methylene Blue Acid - Fast Cells Red Non Acid - Fast BlueSlide 20: Special Stains Capsule Stain Klebsiella pneumoniaeSlide 21: Flagella Stain Spirillum volutansSlide 22: Endospore Stain Bacillus cereusSlide 23: Clostridium botulinum You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
Staining jitpatel21 Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 105 Category: Science & Tech.. License: All Rights Reserved Like it (0) Dislike it (0) Added: July 12, 2011 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Observing Microorganisms through a Microscope: Observing Microorganisms through a Microscope Chapter 3Basic techniques needed to study Bacteria: Basic techniques needed to study Bacteria 1. Grow Bacteria 2. Isolate Bacteria 3. Grow Bacteria in pure culture 4. Observe Bacteria 5. Identify BacteriaMicroscope: Microscope Resolving Power - ability to distinguish two distinct points absolute limit of the Resolving Power is about 1/2 the wavelength of light that is used to illuminate the specimenPreparing smears for staining: Preparing smears for staining 1. Bacteria on slide 2. Air Dry 3. Bacteria are HEAT FIXED to the slide 4. Stain is appliedStaining Reaction: Staining Reaction Stains - salts composed of a positive and negative ion, one of which is colored (chromophore) Basic Dyes - chromophore is the positive ion dye+ Cl- Acid Dyes - chromophore is the negative ion Na+ dye-Bacteria are slightly negative, so are attracted to the positive chromophore of the BASIC DYE: Bacteria are slightly negative, so are attracted to the positive chromophore of the BASIC DYE Common Basic Dyes crystal violet methylene blue safranin basic fuchsinSlide 7: Acid Dyes - used for Negative Staining (background is stained) Mordant - intensifies the stain or coats a structure to make it thicker and easier to see after it is stained Example: Flagella - can not normally be seen, but a mordant can be used to increase the diameter of the flagella before it is stained Salmonella typhosaDifferential Stains: Differential Stains React differently with different types of bacteria 2 Most Common Gram Stain Acid-Fast StainGram Stain: Gram Stain 1884 Hans Christian Gram most important stain used in Bacteriology Divides all Bacteria into 2 groups: Gram (+) Gram (-)Slide 10: Gram Stain 1. Crystal violetSlide 11: Gram Stain 2. Grams Iodine (mordant)Slide 12: Gram Stain 3. AlcoholSlide 13: Gram Stain 4. Safranin (Counterstain)Results: Results Gram (+) Purple Gram (-) Red Difference - due to structure of cell wall Gram (+) Thick cell wall Gram (-) Thin cell wallIdentification of a Bacteria Unknown: Identification of a Bacteria Unknown 1. Gram Reaction 2. MorphologyAcid - Fast Stain: Acid - Fast Stain Differential Stain - divides bacteria into 2 groups Acid - Fast Non Acid - Fast Used to identify organisms in the Genera Mycobacterium (high lipid and wax content in cell wall)Slide 17: 2 Important Pathogens Mycobacterium tuberculosisSlide 18: Mycobacterium lepraeAcid - Fast Stain: Acid - Fast Stain 1. Carbolfuchsin (Red) 2. Acid Alcohol 3. Counterstain with Methylene Blue Acid - Fast Cells Red Non Acid - Fast BlueSlide 20: Special Stains Capsule Stain Klebsiella pneumoniaeSlide 21: Flagella Stain Spirillum volutansSlide 22: Endospore Stain Bacillus cereusSlide 23: Clostridium botulinum