Chromium

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Isolation and Phylogentic identification of Pseudomonas sp. From soil of Amrithi forest region and Checked for its Efficiency in Chromium RemovalB.Samuel Raj VIT University. : 

Isolation and Phylogentic identification of Pseudomonas sp. From soil of Amrithi forest region and Checked for its Efficiency in Chromium RemovalB.Samuel Raj VIT University.

AIM and OBJECTIVES : 

AIM and OBJECTIVES Objectives: To isolate the Pseudomonas sp, by using enrichment method. Further phylogenetically identify the organism using 16S rRNA gene sequence analysis, To determine the efficiency of the isolates in chromium degradation (hexavalent chromium reduction)

METHODLOGY : 

METHODLOGY Sampling Isolation Biochemical tests 16s rRna gene sequencing Chromium degradation Effect of Cr (VI) on bacterial growth Minimum inhibitory concentration (MIC) of Chromate (Broth dilution method) Chromium (VI) reduction study at different pH Determination of Cr(VI) in supernatant solution

RESULTS : 

RESULTS

Slide 8: 

CHARACTERIZATION The 16S rRNA results suggested us that the culture SP8 and SP2 belongs to genus Pseudomonas of Pseudomonadaceae family having the nearest neighbour as Pseudomonas putida (Acc. No. 1224373) with 98.4% similarity and Pseudomonas plecoglossicida (Acc. No. 1224379) with 98.3% similarity.

Slide 10: 

Effect of Cr (VI) on bacterial growth

Slide 11: 

Growth of SP2 With and Without Cr

Slide 12: 

Growth of SP8 Without Cr(VI) and With Cr(VI)

Slide 14: 

Minimum inhibitory concentration (MIC) of Chromium(VI) by broth dilution method

Slide 16: 

Reduction of Cr(VI) at different pH by SP8

Slide 19: 

Determination of Cr(VI) in supernatant solution

Slide 20: 

Dicussion The isolated organism is phylogenetically identified as Pseudomonas putida and Pseudomonas plecoglossicida Based on the MIC Pseudomonas putida is selected and checked for its efficiency in chromium removal It confirm that Pseudomonas putida was capable of removing 90% chromium

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