Presentation Transcript
Slide 1:The Muth lab at Brooklyn College:
Nycol, Rosanna, Monica, Justin, Anna, Karla, Lauren, Hafsa, Emma and Sid.
Slide 2:M. Hawes, University of Arizona Agrobacterium tumefaciens: Host Pathogen Interactions
Slide 4:Infection of plant cells by A. tumefaciens Tzfira and Citovsky 2006 Current Opinion in. Biotechnology Not to scale!
Slide 5:A. tumefaciens can be used to deliver foreign genes to plants Tumorigenic Native T-DNA ~10-20 kb The tumorigenic genes of the T-DNA can be replaced with any gene of interest Modified T-DNA
Slide 6:Current projects:
Identifying A. tumefaciens receptor(s)
Timing of T-DNA integration into the plant genome
Screen for soil bacteria that disrupt wound signaling (biocontrol)
Questions that might be addressed in future projects:
What can explain the low percentage of virulent Agrobacteria in soil samples?
Can we detect tumor-free infections of plant roots in the environment?
Do mycorrhizae inhibit infection by Agrobacteria?
Can certain insects serve as vectors for Agrobacteria infection?
We are looking for collaborators on these
projects or ideas for related projects.
Slide 9:Nonhomologous insertion of DNA vs. gene targeting in plants Plant Cell Nucleus Integration via NHEJ at double strand breaks. Introduced DNA Integration of target gene at target site via homologous recombination. double strand break target site Gene targeting is 1,000-10,000 times less efficient than insertion at double strand DNA breaks target site target site NHEJ GT
Slide 10:CUNY Brooklyn College
Katherine Bernal
Khudeja Mir
Anna Petrovicheva
Lourdianie Pierre-Charles
Esan Wilkinson
The Weizmann Institute
Avraham Levy
Cathy Melamed-Bessudo
Purdue University
Stanton Gelvin
Lan-Ying Lee Acknowledgements: NSF (grants 0633490 and 0520994)
US Dept. of Agriculture (grant 490678)
NIH/NIGMS MARC Program
PSC-CUNY BARD (grant FR-22-2007)